(data stored in ACNUC7421 zone)

SWISSPROT: RPOB_BACSU

ID   RPOB_BACSU              Reviewed;        1193 AA.
AC   P37870;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   11-DEC-2019, entry version 141.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; Synonyms=crsE, rfm;
GN   OrderedLocusNames=BSU01070;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7657605; DOI=10.1074/jbc.270.35.20329;
RA   Boor K.J., Duncan M.L., Price C.W.;
RT   "Genetic and transcriptional organization of the region encoding the beta
RT   subunit of Bacillus subtilis RNA polymerase.";
RL   J. Biol. Chem. 270:20329-20336(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 1073-1074.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   MUTAGENESIS TO STREPTOLYDIGAN RESISTANCE.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7592585; DOI=10.1074/jbc.270.41.23930;
RA   Yang X., Price C.W.;
RT   "Streptolydigin resistance can be conferred by alterations to either the
RT   beta or beta' subunits of Bacillus subtilis RNA polymerase.";
RL   J. Biol. Chem. 270:23930-23933(1995).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA-
CC         COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
DR   EMBL; L24376; AAB00972.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11883.2; -; Genomic_DNA.
DR   PIR; F69698; F69698.
DR   RefSeq; NP_387988.2; NC_000964.3.
DR   RefSeq; WP_009966326.1; NZ_JNCM01000029.1.
DR   PDB; 2LY7; NMR; -; A=784-923.
DR   PDBsum; 2LY7; -.
DR   SMR; P37870; -.
DR   DIP; DIP-49021N; -.
DR   IntAct; P37870; 12.
DR   MINT; P37870; -.
DR   STRING; 224308.BSU01070; -.
DR   jPOST; P37870; -.
DR   PaxDb; P37870; -.
DR   PRIDE; P37870; -.
DR   EnsemblBacteria; CAB11883; CAB11883; BSU01070.
DR   GeneID; 936335; -.
DR   KEGG; bsu:BSU01070; -.
DR   PATRIC; fig|224308.179.peg.110; -.
DR   eggNOG; ENOG4108IIJ; Bacteria.
DR   eggNOG; COG0085; LUCA.
DR   HOGENOM; HOG000218611; -.
DR   InParanoid; P37870; -.
DR   KO; K03043; -.
DR   OMA; FMTWEGY; -.
DR   PhylomeDB; P37870; -.
DR   BioCyc; BSUB:BSU01070-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P37870.
DR   SWISS-2DPAGE; P37870.
KW   3D-structure; Antibiotic resistance; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
FT   CHAIN           1..1193
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047860"
FT   VARIANT         482
FT                   /note="H -> Y (in rfm2103; rifampicin resistant)"
FT   MUTAGEN         499..502
FT                   /note="Missing: Not streptolydigan resistant."
FT                   /evidence="ECO:0000269|PubMed:7592585"
FT   MUTAGEN         499
FT                   /note="A->V: Streptolydigan resistant."
FT                   /evidence="ECO:0000269|PubMed:7592585"
FT   MUTAGEN         500
FT                   /note="G->R: Streptolydigan resistant."
FT                   /evidence="ECO:0000269|PubMed:7592585"
FT   MUTAGEN         501
FT                   /note="M->S: Not streptolydigan resistant."
FT                   /evidence="ECO:0000269|PubMed:7592585"
FT   MUTAGEN         502
FT                   /note="E->V: Streptolydigan resistant."
FT                   /evidence="ECO:0000269|PubMed:7592585"
FT   CONFLICT        1073..1074
FT                   /note="QR -> HG (in Ref. 1; AAB00972)"
FT                   /evidence="ECO:0000305"
FT   STRAND          792..795
FT                   /evidence="ECO:0000244|PDB:2LY7"
FT   TURN            818..821
FT                   /evidence="ECO:0000244|PDB:2LY7"
FT   STRAND          846..848
FT                   /evidence="ECO:0000244|PDB:2LY7"
FT   HELIX           856..865
FT                   /evidence="ECO:0000244|PDB:2LY7"
FT   STRAND          872..874
FT                   /evidence="ECO:0000244|PDB:2LY7"
FT   STRAND          884..893
FT                   /evidence="ECO:0000244|PDB:2LY7"
FT   HELIX           895..897
FT                   /evidence="ECO:0000244|PDB:2LY7"
FT   STRAND          908..915
FT                   /evidence="ECO:0000244|PDB:2LY7"
SQ   SEQUENCE   1193 AA;  133686 MW;  713FFF19F0987443 CRC64;
     MTGQLVQYGR HRQRRSYARI SEVLELPNLI EIQTSSYQWF LDEGLREMFQ DISPIEDFTG
     NLSLEFIDYS LGEPKYPVEE SKERDVTYSA PLRVKVRLIN KETGEVKDQD VFMGDFPIMT
     DTGTFIINGA ERVIVSQLVR SPSVYFSGKV DKNGKKGFTA TVIPNRGAWL EYETDAKDVV
     YVRIDRTRKL PVTVLLRALG FGSDQEILDL IGENEYLRNT LDKDNTENSD KALLEIYERL
     RPGEPPTVEN AKSLLDSRFF DPKRYDLANV GRYKINKKLH IKNRLFNQRL AETLVDPETG
     EILAEKGQIL DRRTLDKVLP YLENGIGFRK LYPNGGVVED EVTLQSIKIF APTDQEGEQV
     INVIGNAYIE EEIKNITPAD IISSISYFFN LLHGVGDTDD IDHLGNRRLR SVGELLQNQF
     RIGLSRMERV VRERMSIQDT NTITPQQLIN IRPVIASIKE FFGSSQLSQF MDQTNPLAEL
     THKRRLSALG PGGLTRERAG MEVRDVHYSH YGRMCPIETP EGPNIGLINS LSSYAKVNRF
     GFIETPYRRV DPETGKVTGR IDYLTADEED NYVVAQANAR LDDEGAFIDD SIVARFRGEN
     TVVSRNRVDY MDVSPKQVVS AATACIPFLE NDDSNRALMG ANMQRQAVPL MQPEAPFVGT
     GMEYVSGKDS GAAVICKHPG IVERVEAKNV WVRRYEEVDG QKVKGNLDKY SLLKFVRSNQ
     GTCYNQRPIV SVGDEVVKGE ILADGPSMEL GELALGRNVM VGFMTWDGYN YEDAIIMSER
     LVKDDVYTSI HIEEYESEAR DTKLGPEEIT RDIPNVGEDA LRNLDDRGII RIGAEVKDGD
     LLVGKVTPKG VTELTAEERL LHAIFGEKAR EVRDTSLRVP HGGGGIIHDV KVFNREDGDE
     LPPGVNQLVR VYIVQKRKIS EGDKMAGRHG NKGVISKILP EEDMPYLPDG TPIDIMLNPL
     GVPSRMNIGQ VLELHMGMAA RYLGIHIASP VFDGAREEDV WETLEEAGMS RDAKTVLYDG
     RTGEPFDNRV SVGIMYMIKL AHMVDDKLHA RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV
     WALEAYGAAY TLQEILTVKS DDVVGRVKTY EAIVKGDNVP EPGVPESFKV LIKELQSLGM
     DVKILSGDEE EIEMRDLEDE EDAKQADGLA LSGDEEPEET ASADVERDVV TKE
//

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