(data stored in ACNUC7421 zone)

SWISSPROT: RPOC_BACSU

ID   RPOC_BACSU              Reviewed;        1199 AA.
AC   P37871; P70978;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 4.
DT   11-DEC-2019, entry version 135.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE            Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE   AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE   AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
GN   Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322}; Synonyms=lpm, std;
GN   OrderedLocusNames=BSU01080;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND STREPTOLYDIGAN RESISTANCE.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7592585; DOI=10.1074/jbc.270.41.23930;
RA   Yang X., Price C.W.;
RT   "Streptolydigin resistance can be conferred by alterations to either the
RT   beta or beta' subunits of Bacillus subtilis RNA polymerase.";
RL   J. Biol. Chem. 270:23930-23933(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 1026; 1173 AND 1176.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-466.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7657605; DOI=10.1074/jbc.270.35.20329;
RA   Boor K.J., Duncan M.L., Price C.W.;
RT   "Genetic and transcriptional organization of the region encoding the beta
RT   subunit of Bacillus subtilis RNA polymerase.";
RL   J. Biol. Chem. 270:20329-20336(1995).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 453-1199.
RC   STRAIN=168;
RX   PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA   Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA   Kawamura F., Yoshikawa H., Takahashi H.;
RT   "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT   subtilis chromosome.";
RL   Microbiology 142:3039-3046(1996).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01322}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA-
CC         COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_01322};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01322}.
CC   -!- INTERACTION:
CC       C0H3R4:rsoA; NbExp=3; IntAct=EBI-5244361, EBI-15816929;
CC       O31602:spxA; NbExp=2; IntAct=EBI-5244361, EBI-5248631;
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01322}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB59112.1; Type=Frameshift; Evidence={ECO:0000305};
DR   EMBL; L43593; AAB59112.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AL009126; CAB11884.2; -; Genomic_DNA.
DR   EMBL; L24376; AAB00973.1; -; Genomic_DNA.
DR   EMBL; D64127; BAA10999.1; -; Genomic_DNA.
DR   PIR; G69698; G69698.
DR   RefSeq; NP_387989.2; NC_000964.3.
DR   RefSeq; WP_004399688.1; NZ_JNCM01000029.1.
DR   SMR; P37871; -.
DR   DIP; DIP-49022N; -.
DR   IntAct; P37871; 7.
DR   MINT; P37871; -.
DR   STRING; 224308.BSU01080; -.
DR   jPOST; P37871; -.
DR   PaxDb; P37871; -.
DR   PRIDE; P37871; -.
DR   EnsemblBacteria; CAB11884; CAB11884; BSU01080.
DR   GeneID; 935977; -.
DR   KEGG; bsu:BSU01080; -.
DR   PATRIC; fig|224308.179.peg.111; -.
DR   eggNOG; ENOG4105D27; Bacteria.
DR   eggNOG; COG0086; LUCA.
DR   HOGENOM; HOG000218387; -.
DR   InParanoid; P37871; -.
DR   KO; K03046; -.
DR   OMA; QGVEIND; -.
DR   PhylomeDB; P37871; -.
DR   BioCyc; BSUB:BSU01080-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR   InterPro; IPR012754; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
DR   TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P37871.
DR   SWISS-2DPAGE; P37871.
KW   Antibiotic resistance; DNA-directed RNA polymerase; Magnesium;
KW   Metal-binding; Nucleotidyltransferase; Reference proteome; Transcription;
KW   Transferase; Zinc.
FT   CHAIN           1..1199
FT                   /note="DNA-directed RNA polymerase subunit beta'"
FT                   /id="PRO_0000067707"
FT   METAL           60
FT                   /note="Zinc 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           62
FT                   /note="Zinc 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           75
FT                   /note="Zinc 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           78
FT                   /note="Zinc 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           449
FT                   /note="Magnesium"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           451
FT                   /note="Magnesium"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           453
FT                   /note="Magnesium"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           818
FT                   /note="Zinc 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           892
FT                   /note="Zinc 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           899
FT                   /note="Zinc 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   METAL           902
FT                   /note="Zinc 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   VARIANT         796
FT                   /note="D -> G (in streptolydigan resistant alleles stl6/
FT                   stl445)"
FT   CONFLICT        781
FT                   /note="H -> P (in Ref. 5; BAA10999)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1026
FT                   /note="E -> G (in Ref. 5; BAA10999)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1173
FT                   /note="A -> G (in Ref. 5; BAA10999)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1176
FT                   /note="G -> A (in Ref. 5; BAA10999)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1199 AA;  134252 MW;  33263510698FC12B CRC64;
     MLDVNNFEYM NIGLASPDKI RSWSFGEVKK PETINYRTLK PEKDGLFCER IFGPTKDWEC
     HCGKYKRVRY KGVVCDRCGV EVTRAKVRRE RMGHIELAAP VSHIWYFKGI PSRMGLVLDM
     SPRALEEVIY FASYVVTDPA NTPLEKKQLL SEKEYRAYLD KYGNKFQASM GAEAIHKLLQ
     DIDLVKEVDM LKEELKTSQG QRRTRAIKRL EVLEAFRNSG NKPSWMILDV LPVIPPELRP
     MVQLDGGRFA TSDLNDLYRR VINRNNRLKR LLDLGAPSII VQNEKRMLQE AVDALIDNGR
     RGRPVTGPGN RPLKSLSHML KGKQGRFRQN LLGKRVDYSG RSVIVVGPHL KMYQCGLPKE
     MALELFKPFV MKELVEKGLA HNIKSAKRKI ERVQPEVWDV LESVIKEHPV LLNRAPTLHR
     LGIQAFEPTL VEGRAIRLHP LVCTAYNADF DGDQMAVHVP LSAEAQAEAR ILMLAAQNIL
     NPKDGKPVVT PSQDMVLGNY YLTLERAGAV GEGMVFKNTD EALLAYQNGY VHLHTRVAVA
     ANSLKNVTFT EEQRSKLLIT TVGKLVFNEI LPESFPYMNE PTKSNIEEKT PDRFFLEKGA
     DVKAVIAQQP INAPFKKGIL GKIIAEIFKR FHITETSKML DRMKNLGFKY STKAGITVGV
     SDIVVLDDKQ EILEEAQSKV DNVMKQFRRG LITEEERYER VISIWSAAKD VIQGKLMKSL
     DELNPIYMMS DSGARGNASN FTQLAGMRGL MANPAGRIIE LPIKSSFREG LTVLEYFIST
     HGARKGLADT ALKTADSGYL TRRLVDVAQD VIIRETDCGT DRGILAKPLK EGTETIERLE
     ERLIGRFARK QVKHPETGEV LVNENELIDE DKALEIVEAG IEEVWIRSAF TCNTPHGVCK
     RCYGRNLATG SDVEVGEAVG IIAAQSIGEP GTQLTMRTFH TGGVAGDDIT QGLPRIQELF
     EARNPKGQAT ITEIDGTVVE INEVRDKQQE IVVQGAVETR SYTAPYNSRL KVAEGDKITR
     GQVLTEGSID PKELLKVTDL TTVQEYLLHE VQKVYRMQGV EIGDKHVEVM VRQMLRKVRV
     IDAGDTDVLP GTLLDIHQFT EANKKVLLEG NRPATGRPVL LGITKASLET DSFLSAASFQ
     ETTRVLTDAA IKGKRDELLG LKENVIIGKL VPAGTGMMKY RKVKPVSNVQ PTDDMVPVE
//

If you have problems or comments...

PBIL Back to PBIL home page