(data stored in SCRATCH zone)

SWISSPROT: RXL7_BACSU

ID   RXL7_BACSU              Reviewed;          82 AA.
AC   P46350;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   26-JUL-2002, sequence version 3.
DT   11-DEC-2019, entry version 125.
DE   RecName: Full=Ribosome-associated protein L7Ae-like;
GN   Name=rplGB; Synonyms=ybaB, ybxF; OrderedLocusNames=BSU01090;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7657605; DOI=10.1074/jbc.270.35.20329;
RA   Boor K.J., Duncan M.L., Price C.W.;
RT   "Genetic and transcriptional organization of the region encoding the beta
RT   subunit of Bacillus subtilis RNA polymerase.";
RL   J. Biol. Chem. 270:20329-20336(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA   Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA   Kawamura F., Yoshikawa H., Takahashi H.;
RT   "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT   subtilis chromosome.";
RL   Microbiology 142:3039-3046(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   RIBOSOME ASSOCIATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF LYS-17;
RP   LYS-21 AND LYS-24.
RC   STRAIN=168;
RX   PubMed=17468242; DOI=10.1128/jb.01786-06;
RA   Sojka L., Fucik V., Krasny L., Barvik I., Jonak J.;
RT   "YbxF, a protein associated with exponential-phase ribosomes in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 189:4809-4814(2007).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH RNA, FUNCTION, AND
RP   RNA-BINDING.
RX   PubMed=22355167; DOI=10.1261/rna.031518.111;
RA   Baird N.J., Zhang J., Hamma T., Ferre-D'Amare A.R.;
RT   "YbxF and YlxQ are bacterial homologs of L7Ae and bind K-turns but not K-
RT   loops.";
RL   RNA 18:759-770(2012).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH K-TURN RNA.
RX   PubMed=23892783; DOI=10.1038/nature12440;
RA   Zhang J., Ferre-D'Amare A.R.;
RT   "Co-crystal structure of a T-box riboswitch stem I domain in complex with
RT   its cognate tRNA.";
RL   Nature 500:363-366(2013).
CC   -!- FUNCTION: RNA-binding protein that recognizes the K-turn motif present
CC       in ribosomal RNA, but also in box C/D and box C'/D' sRNAs.
CC       {ECO:0000269|PubMed:22355167}.
CC   -!- SUBUNIT: Found associated with the 50S subunit of the ribosome during
CC       exponential growth but not during stationary phase.
CC       {ECO:0000269|PubMed:22355167, ECO:0000269|PubMed:23892783}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Note=Localizes to the cell pole in
CC       exponential growth.
CC   -!- DISRUPTION PHENOTYPE: None; has no effect on viability, growth or
CC       sporulation at low, normal or elevated temperatures.
CC       {ECO:0000269|PubMed:17468242}.
CC   -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eL8 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00574}.
DR   EMBL; L43593; AAB59113.1; -; Genomic_DNA.
DR   EMBL; D64127; BAA11000.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11885.1; -; Genomic_DNA.
DR   PIR; G69751; G69751.
DR   RefSeq; NP_387990.1; NC_000964.3.
DR   RefSeq; WP_003225778.1; NZ_JNCM01000029.1.
DR   PDB; 3V7E; X-ray; 2.80 A; A/B=2-82.
DR   PDB; 4LCK; X-ray; 3.20 A; A/D=2-82.
DR   PDB; 4TZP; X-ray; 8.50 A; A/D=2-82.
DR   PDB; 4TZV; X-ray; 5.03 A; A=2-82.
DR   PDB; 4TZW; X-ray; 4.67 A; A=2-82.
DR   PDB; 4TZZ; X-ray; 3.64 A; A/D/G/J=2-82.
DR   PDBsum; 3V7E; -.
DR   PDBsum; 4LCK; -.
DR   PDBsum; 4TZP; -.
DR   PDBsum; 4TZV; -.
DR   PDBsum; 4TZW; -.
DR   PDBsum; 4TZZ; -.
DR   SMR; P46350; -.
DR   STRING; 224308.BSU01090; -.
DR   PaxDb; P46350; -.
DR   PRIDE; P46350; -.
DR   EnsemblBacteria; CAB11885; CAB11885; BSU01090.
DR   GeneID; 936829; -.
DR   KEGG; bsu:BSU01090; -.
DR   PATRIC; fig|224308.179.peg.112; -.
DR   eggNOG; ENOG4105XPM; Bacteria.
DR   eggNOG; COG1358; LUCA.
DR   HOGENOM; HOG000055225; -.
DR   InParanoid; P46350; -.
DR   KO; K07590; -.
DR   OMA; YIDTMKE; -.
DR   BioCyc; BSUB:BSU01090-MONOMER; -.
DR   PRO; PR:P46350; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IEA:InterPro.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   HAMAP; MF_00574; Ribosomal_L7Ae_Bact; 1.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
DR   InterPro; IPR000948; Ribosomal_L7Ae_prok.
DR   InterPro; IPR023460; Ribosomal_L7Ae_putative.
DR   Pfam; PF01248; Ribosomal_L7Ae; 1.
DR   PRINTS; PR00884; RIBOSOMALHS6.
DR   SUPFAM; SSF55315; SSF55315; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P46350.
DR   SWISS-2DPAGE; P46350.
KW   3D-structure; Cytoplasm; Reference proteome; Ribonucleoprotein;
KW   Ribosomal protein; RNA-binding.
FT   CHAIN           1..82
FT                   /note="Ribosome-associated protein L7Ae-like"
FT                   /id="PRO_0000136812"
FT   MUTAGEN         17
FT                   /note="K->A: No effect."
FT                   /evidence="ECO:0000269|PubMed:17468242"
FT   MUTAGEN         21
FT                   /note="K->A: No effect."
FT                   /evidence="ECO:0000269|PubMed:17468242"
FT   MUTAGEN         24
FT                   /note="K->A: Decreased ribosomal association."
FT                   /evidence="ECO:0000269|PubMed:17468242"
FT   HELIX           3..8
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   STRAND          10..15
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   HELIX           16..23
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   TURN            24..26
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   STRAND          28..34
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   HELIX           39..52
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   STRAND          56..60
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   HELIX           62..68
FT                   /evidence="ECO:0000244|PDB:3V7E"
FT   STRAND          76..81
FT                   /evidence="ECO:0000244|PDB:3V7E"
SQ   SEQUENCE   82 AA;  8462 MW;  B8E301C141E77211 CRC64;
     MSYDKVSQAK SIIIGTKQTV KALKRGSVKE VVVAKDADPI LTSSVVSLAE DQGISVSMVE
     SMKKLGKACG IEVGAAAVAI IL
//

If you have problems or comments...

PBIL Back to PBIL home page