(data stored in ACNUC7421 zone)

SWISSPROT: RS12_BACSU

ID   RS12_BACSU              Reviewed;         138 AA.
AC   P21472;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 5.
DT   11-DEC-2019, entry version 146.
DE   RecName: Full=30S ribosomal protein S12;
DE            Short=BS12;
GN   Name=rpsL; Synonyms=fun, strA; OrderedLocusNames=BSU01100;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA   Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA   Kawamura F., Yoshikawa H., Takahashi H.;
RT   "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT   subtilis chromosome.";
RL   Microbiology 142:3039-3046(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 102; 105 AND 128, AND LACK OF MODIFICATION.
RC   STRAIN=168;
RA   Ochi K., Takashi I., Forbes A., Kelleher N.L., Garavelli J.S.;
RL   Unpublished observations (AUG-2002).
RN   [4]
RP   SEQUENCE REVISION TO 102 AND 105.
RC   STRAIN=168 / BGSC1A1, and ATCC 21332 / IAM 1213;
RX   PubMed=16484214; DOI=10.1128/jb.188.5.2020-2023.2006;
RA   Carr J.F., Hamburg D.M., Gregory S.T., Limbach P.A., Dahlberg A.E.;
RT   "Effects of streptomycin resistance mutations on posttranslational
RT   modification of ribosomal protein S12.";
RL   J. Bacteriol. 188:2020-2023(2006).
RN   [5]
RP   SEQUENCE REVISION TO 102; 105 AND 128, AND LACK OF MODIFICATION.
RC   STRAIN=168;
RX   PubMed=19653700; DOI=10.1021/pr801114k;
RA   Lauber M.A., Running W.E., Reilly J.P.;
RT   "B. subtilis ribosomal proteins: structural homology and post-translational
RT   modifications.";
RL   J. Proteome Res. 8:4193-4206(2009).
RN   [6]
RP   SEQUENCE REVISION TO 102; 105 AND 128.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7657605; DOI=10.1074/jbc.270.35.20329;
RA   Boor K.J., Duncan M.L., Price C.W.;
RT   "Genetic and transcriptional organization of the region encoding the beta
RT   subunit of Bacillus subtilis RNA polymerase.";
RL   J. Biol. Chem. 270:20329-20336(1995).
RN   [8]
RP   PROTEIN SEQUENCE OF 2-21.
RX   PubMed=6806564; DOI=10.1007/bf00330792;
RA   Higo K., Otaka E., Osawa S.;
RT   "Purification and characterization of 30S ribosomal proteins from Bacillus
RT   subtilis: correlation to Escherichia coli 30S proteins.";
RL   Mol. Gen. Genet. 185:239-244(1982).
RN   [9]
RP   VARIANTS STREPTOMYCIN RESISTANT, AND ANTIBIOTIC PRODUCTION.
RC   STRAIN=168;
RX   PubMed=9687404; DOI=10.1128/aac.42.8.2041;
RA   Hosoya Y., Okamoto S., Muramatsu H., Ochi K.;
RT   "Acquisition of certain streptomycin-resistant (str) mutations enhances
RT   antibiotic production in bacteria.";
RL   Antimicrob. Agents Chemother. 42:2041-2047(1998).
RN   [10]
RP   CHARACTERIZATION, MUTAGENESIS, AND VARIANTS STREPTOMYCIN RESISTANT.
RC   STRAIN=168;
RX   PubMed=11489846; DOI=10.1128/jb.183.17.4958-4963.2001;
RA   Inaoka T., Kasai K., Ochi K.;
RT   "Construction of an in vivo nonsense readthrough assay system and
RT   functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 183:4958-4963(2001).
CC   -!- FUNCTION: With S4 and S5 plays an important role in translational
CC       accuracy.
CC   -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC       involved in tRNA selection in the A site and with the mRNA backbone.
CC       Located at the interface of the 30S and 50S subunits, it traverses the
CC       body of the 30S subunit contacting proteins on the other side and
CC       probably holding the rRNA structure together. The combined cluster of
CC       proteins S8, S12 and S17 appears to hold together the shoulder and
CC       platform of the 30S subunit (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC       S17. May interact with IF1 in the 30S initiation complex (By
CC       similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Many streptomycin resistant B.subtilis with mutations in
CC       this protein produce increased quantities of a natural (but
CC       uncharacterized) antibiotic.
CC   -!- MISCELLANEOUS: A number of substitutions and deletions can promote
CC       streptomycin resistance, dependence or pseudodependence; all but one of
CC       these (K55R) are associated with hyperaccurate translation.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The enzyme that would modify Asp-102 to 3-methylthioaspartic
CC       acid has not been found in the proteome of this organism, and that
CC       modification does not occur. {ECO:0000305}.
DR   EMBL; D64127; BAA11001.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11886.2; -; Genomic_DNA.
DR   EMBL; L43593; AAB59114.1; -; Genomic_DNA.
DR   PIR; C69700; C69700.
DR   RefSeq; NP_387991.2; NC_000964.3.
DR   RefSeq; WP_003225781.1; NZ_JNCM01000029.1.
DR   PDB; 3J9W; EM; 3.90 A; AL=1-138.
DR   PDB; 5NJT; EM; 3.80 A; L=2-138.
DR   PDB; 6HA1; EM; 3.10 A; l=1-138.
DR   PDB; 6HA8; EM; 3.50 A; l=1-138.
DR   PDBsum; 3J9W; -.
DR   PDBsum; 5NJT; -.
DR   PDBsum; 6HA1; -.
DR   PDBsum; 6HA8; -.
DR   SMR; P21472; -.
DR   STRING; 224308.BSU01100; -.
DR   PaxDb; P21472; -.
DR   PRIDE; P21472; -.
DR   EnsemblBacteria; CAB11886; CAB11886; BSU01100.
DR   GeneID; 936616; -.
DR   KEGG; bsu:BSU01100; -.
DR   PATRIC; fig|224308.179.peg.113; -.
DR   eggNOG; ENOG4108UKE; Bacteria.
DR   eggNOG; COG0048; LUCA.
DR   HOGENOM; HOG000040063; -.
DR   InParanoid; P21472; -.
DR   KO; K02950; -.
DR   OMA; SPALEKC; -.
DR   PhylomeDB; P21472; -.
DR   BioCyc; BSUB:BSU01100-MONOMER; -.
DR   PRO; PR:P21472; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IBA:GO_Central.
DR   CDD; cd03368; Ribosomal_S12; 1.
DR   HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006032; Ribosomal_S12/S23.
DR   InterPro; IPR005679; Ribosomal_S12_bac.
DR   PANTHER; PTHR11652; PTHR11652; 1.
DR   Pfam; PF00164; Ribosom_S12_S23; 1.
DR   PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR   PRINTS; PR01034; RIBOSOMALS12.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR   PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P21472.
DR   SWISS-2DPAGE; P21472.
KW   3D-structure; Antibiotic resistance; Direct protein sequencing;
KW   Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW   rRNA-binding; tRNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:6806564"
FT   CHAIN           2..138
FT                   /note="30S ribosomal protein S12"
FT                   /id="PRO_0000146178"
FT   SITE            102
FT                   /note="Not modified"
FT                   /evidence="ECO:0000269|PubMed:19653700, ECO:0000269|Ref.3"
FT   VARIANT         56
FT                   /note="K -> I (in RPSL4 (STRA1); confers streptomycin
FT                   resistance and hyperaccurate translation, has no effect on
FT                   natural antibiotic production)"
FT   VARIANT         56
FT                   /note="K -> R (in RPSL1 (KO-267); confers streptomycin
FT                   resistance but not hyperaccurate translation, increases
FT                   natural antibiotic production 10-fold)"
FT   MUTAGEN         56
FT                   /note="K->D: Confers streptomycin resistance and very poor
FT                   growth in rich medium."
FT                   /evidence="ECO:0000269|PubMed:11489846"
FT   CONFLICT        102
FT                   /note="D -> N (in Ref. 1; BAA11001)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        105
FT                   /note="G -> R (in Ref. 1; BAA11001)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        128
FT                   /note="S -> P (in Ref. 1; BAA11001)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   138 AA;  15216 MW;  1E8EDCEC75B75648 CRC64;
     MPTINQLIRK GRVSKVENSK SPALNKGYNS FKKEHTNVSS PQKRGVCTRV GTMTPKKPNS
     ALRKYARVRL TNGIEVTAYI PGIGHNLQEH SVVLIRGGRV KDLPGVRYHI VRGALDTAGV
     ENRAQGRSKY GTKKPKAK
//

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