(data stored in ACNUC7421 zone)

SWISSPROT: RS14_BACSU

ID   RS14_BACSU              Reviewed;          61 AA.
AC   P12878;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   11-DEC-2019, entry version 135.
DE   RecName: Full=30S ribosomal protein S14;
DE   AltName: Full=30S ribosomal protein S14 type Z {ECO:0000255|HAMAP-Rule:MF_01364};
DE   AltName: Full=30S ribosomal protein S14-1;
DE   AltName: Full=BS-A;
GN   Name=rpsN1;
GN   Synonyms=rpsN {ECO:0000255|HAMAP-Rule:MF_01364},
GN   rpsNA {ECO:0000255|HAMAP-Rule:MF_01364}, rpsZ; OrderedLocusNames=BSU01290;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=2508062; DOI=10.1093/nar/17.18.7469;
RA   Henkin T.M., Moon S.H., Mattheakis L.C., Nomura M.;
RT   "Cloning and analysis of the spc ribosomal protein operon of Bacillus
RT   subtilis: comparison with the spc operon of Escherichia coli.";
RL   Nucleic Acids Res. 17:7469-7486(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA   Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT   "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT   alpha region.";
RL   Gene 169:17-23(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-22.
RX   PubMed=6806564; DOI=10.1007/bf00330792;
RA   Higo K., Otaka E., Osawa S.;
RT   "Purification and characterization of 30S ribosomal proteins from Bacillus
RT   subtilis: correlation to Escherichia coli 30S proteins.";
RL   Mol. Gen. Genet. 185:239-244(1982).
RN   [5]
RP   DISRUPTION PHENOTYPE, AND IDENTIFICATION IN RIBOSOME COMPLEX.
RC   STRAIN=168;
RX   PubMed=17163968; DOI=10.1111/j.1365-2958.2006.05513.x;
RA   Natori Y., Nanamiya H., Akanuma G., Kosono S., Kudo T., Ochi K.,
RA   Kawamura F.;
RT   "A fail-safe system for the ribosome under zinc-limiting conditions in
RT   Bacillus subtilis.";
RL   Mol. Microbiol. 63:294-307(2007).
CC   -!- FUNCTION: Binds 16S rRNA, required for the assembly of 30S particles
CC       and may also be responsible for determining the conformation of the 16S
CC       rRNA at the A site (By similarity). The major S14 protein in the
CC       ribosome. Required for binding of S2 and S3 to the 30S subunit and for
CC       association of the 30S with the 50S subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_01364}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01364};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01364};
CC   -!- SUBUNIT: Contacts proteins S3 and S10 (By similarity). Part of the 30S
CC       ribosomal subunit. {ECO:0000250, ECO:0000269|PubMed:17163968}.
CC   -!- DISRUPTION PHENOTYPE: Cannot be disrupted; as the protein decreases in
CC       cells 70S ribosome formation decreases rapidly, with concomitant loss
CC       of S2, S3 and YugI proteins from the 30S subunit.
CC       {ECO:0000269|PubMed:17163968}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS14 family.
CC       Zinc-binding uS14 subfamily. {ECO:0000255|HAMAP-Rule:MF_01364}.
DR   EMBL; X15664; CAA33704.1; -; Genomic_DNA.
DR   EMBL; L47971; AAB06812.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11905.1; -; Genomic_DNA.
DR   PIR; S05995; R3BS14.
DR   RefSeq; NP_388010.1; NC_000964.3.
DR   RefSeq; WP_003156488.1; NZ_JNCM01000029.1.
DR   PDB; 3J9W; EM; 3.90 A; AN=1-61.
DR   PDB; 5NJT; EM; 3.80 A; N=2-61.
DR   PDB; 6HA1; EM; 3.10 A; n=1-61.
DR   PDB; 6HA8; EM; 3.50 A; n=1-61.
DR   PDBsum; 3J9W; -.
DR   PDBsum; 5NJT; -.
DR   PDBsum; 6HA1; -.
DR   PDBsum; 6HA8; -.
DR   SMR; P12878; -.
DR   STRING; 224308.BSU01290; -.
DR   PaxDb; P12878; -.
DR   PRIDE; P12878; -.
DR   EnsemblBacteria; CAB11905; CAB11905; BSU01290.
DR   GeneID; 33962971; -.
DR   GeneID; 935946; -.
DR   KEGG; bsu:BSU01290; -.
DR   PATRIC; fig|224308.179.peg.132; -.
DR   eggNOG; ENOG41082QR; Bacteria.
DR   eggNOG; COG0199; LUCA.
DR   HOGENOM; HOG000039865; -.
DR   InParanoid; P12878; -.
DR   KO; K02954; -.
DR   OMA; RAYTRCN; -.
DR   PhylomeDB; P12878; -.
DR   BioCyc; BSUB:BSU01290-MONOMER; -.
DR   PRO; PR:P12878; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01364_B; Ribosomal_S14_2_B; 1.
DR   InterPro; IPR001209; Ribosomal_S14.
DR   InterPro; IPR018271; Ribosomal_S14_CS.
DR   InterPro; IPR023053; Ribosomal_S14_Z.
DR   PANTHER; PTHR19836; PTHR19836; 1.
DR   Pfam; PF00253; Ribosomal_S14; 1.
DR   PROSITE; PS00527; RIBOSOMAL_S14; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P12878.
DR   SWISS-2DPAGE; P12878.
KW   3D-structure; Direct protein sequencing; Metal-binding; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding; Zinc.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:6806564"
FT   CHAIN           2..61
FT                   /note="30S ribosomal protein S14"
FT                   /id="PRO_0000130872"
FT   METAL           24
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01364"
FT   METAL           27
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01364"
FT   METAL           40
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01364"
FT   METAL           43
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01364"
SQ   SEQUENCE   61 AA;  7246 MW;  FC37E9953A148F35 CRC64;
     MAKKSMIAKQ QRTPKFKVQE YTRCERCGRP HSVIRKFKLC RICFRELAYK GQIPGVKKAS
     W
//

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