(data stored in ACNUC7421 zone)

SWISSPROT: RS8_BACSU

ID   RS8_BACSU               Reviewed;         132 AA.
AC   P12879; P70968;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   11-DEC-2019, entry version 133.
DE   RecName: Full=30S ribosomal protein S8 {ECO:0000255|HAMAP-Rule:MF_01302};
DE            Short=BS8;
GN   Name=rpsH {ECO:0000255|HAMAP-Rule:MF_01302}; OrderedLocusNames=BSU01300;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA   Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT   "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT   alpha region.";
RL   Gene 169:17-23(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA   Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA   Kawamura F., Yoshikawa H., Takahashi H.;
RT   "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT   subtilis chromosome.";
RL   Microbiology 142:3039-3046(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   SEQUENCE REVISION TO 37 AND 63.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RC   STRAIN=168;
RX   PubMed=2508062; DOI=10.1093/nar/17.18.7469;
RA   Henkin T.M., Moon S.H., Mattheakis L.C., Nomura M.;
RT   "Cloning and analysis of the spc ribosomal protein operon of Bacillus
RT   subtilis: comparison with the spc operon of Escherichia coli.";
RL   Nucleic Acids Res. 17:7469-7486(1989).
RN   [6]
RP   PROTEIN SEQUENCE OF 2-31.
RX   PubMed=6806564; DOI=10.1007/bf00330792;
RA   Higo K., Otaka E., Osawa S.;
RT   "Purification and characterization of 30S ribosomal proteins from Bacillus
RT   subtilis: correlation to Escherichia coli 30S proteins.";
RL   Mol. Gen. Genet. 185:239-244(1982).
CC   -!- FUNCTION: One of the primary rRNA binding proteins, it binds directly
CC       to 16S rRNA central domain where it helps coordinate assembly of the
CC       platform of the 30S subunit. {ECO:0000255|HAMAP-Rule:MF_01302}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S5 and
CC       S12. {ECO:0000255|HAMAP-Rule:MF_01302}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS8 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01302}.
DR   EMBL; L47971; AAB06813.1; -; Genomic_DNA.
DR   EMBL; D64125; BAA10980.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11906.2; -; Genomic_DNA.
DR   EMBL; X15664; CAA33705.1; -; Genomic_DNA.
DR   PIR; G69699; G69699.
DR   RefSeq; NP_388011.2; NC_000964.3.
DR   RefSeq; WP_003241998.1; NZ_JNCM01000029.1.
DR   PDB; 3J9W; EM; 3.90 A; AH=1-132.
DR   PDB; 5NJT; EM; 3.80 A; H=2-132.
DR   PDB; 6HA1; EM; 3.10 A; h=1-132.
DR   PDB; 6HA8; EM; 3.50 A; h=1-132.
DR   PDBsum; 3J9W; -.
DR   PDBsum; 5NJT; -.
DR   PDBsum; 6HA1; -.
DR   PDBsum; 6HA8; -.
DR   SMR; P12879; -.
DR   STRING; 224308.BSU01300; -.
DR   jPOST; P12879; -.
DR   PaxDb; P12879; -.
DR   PRIDE; P12879; -.
DR   EnsemblBacteria; CAB11906; CAB11906; BSU01300.
DR   GeneID; 936328; -.
DR   KEGG; bsu:BSU01300; -.
DR   PATRIC; fig|224308.179.peg.133; -.
DR   eggNOG; ENOG4108UJY; Bacteria.
DR   eggNOG; COG0096; LUCA.
DR   HOGENOM; HOG000204095; -.
DR   InParanoid; P12879; -.
DR   KO; K02994; -.
DR   OMA; FDQGYIL; -.
DR   PhylomeDB; P12879; -.
DR   BioCyc; BSUB:BSU01300-MONOMER; -.
DR   PRO; PR:P12879; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01302_B; Ribosomal_S8_B; 1.
DR   InterPro; IPR000630; Ribosomal_S8.
DR   InterPro; IPR035987; Ribosomal_S8_sf.
DR   PANTHER; PTHR11758; PTHR11758; 1.
DR   Pfam; PF00410; Ribosomal_S8; 1.
DR   SUPFAM; SSF56047; SSF56047; 1.
DR   PROSITE; PS00053; RIBOSOMAL_S8; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P12879.
DR   SWISS-2DPAGE; P12879.
KW   3D-structure; Direct protein sequencing; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:6806564"
FT   CHAIN           2..132
FT                   /note="30S ribosomal protein S8"
FT                   /id="PRO_0000126367"
FT   CONFLICT        18..19
FT                   /note="NM -> DN (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        30
FT                   /note="S -> B (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        37
FT                   /note="A -> P (in Ref. 2; BAA10980)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        63
FT                   /note="F -> S (in Ref. 2; BAA10980)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        131..132
FT                   /note="Missing (in Ref. 2; BAA10980)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   132 AA;  14877 MW;  B47FA5DAD9F2F055 CRC64;
     MVMTDPIADM LTRIRNANMV RHEKLEIPAS KLKREIAEIL KREGFIRDVE FVEDSKQGII
     RVFLKYGQNN ERVITGLKRI SKPGLRVYAK SNEVPRVLNG LGIAIISTSQ GVLTDKEARA
     KQAGGEVLAY VW
//

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