(data stored in ACNUC7421 zone)

SWISSPROT: RS5_BACSU

ID   RS5_BACSU               Reviewed;         166 AA.
AC   P21467;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 3.
DT   11-DEC-2019, entry version 141.
DE   RecName: Full=30S ribosomal protein S5;
DE            Short=BS5;
GN   Name=rpsE; Synonyms=spcA; OrderedLocusNames=BSU01330;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA   Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT   "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT   alpha region.";
RL   Gene 169:17-23(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT SPECTINOMYCIN RESISTANT.
RC   STRAIN=1A241;
RX   PubMed=2139212; DOI=10.1093/nar/18.6.1647;
RA   Yoshikawa H., Doi R.H.;
RT   "Sequence of the Bacillus subtilis spectinomycin resistance gene region.";
RL   Nucleic Acids Res. 18:1647-1647(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
RC   STRAIN=168;
RX   PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA   Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA   Kawamura F., Yoshikawa H., Takahashi H.;
RT   "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT   subtilis chromosome.";
RL   Microbiology 142:3039-3046(1996).
RN   [5]
RP   PROTEIN SEQUENCE OF 1-40.
RX   PubMed=6806564; DOI=10.1007/bf00330792;
RA   Higo K., Otaka E., Osawa S.;
RT   "Purification and characterization of 30S ribosomal proteins from Bacillus
RT   subtilis: correlation to Escherichia coli 30S proteins.";
RL   Mol. Gen. Genet. 185:239-244(1982).
RN   [6]
RP   PROTEIN SEQUENCE OF 1-46, AND VARIANTS SPECTINOMYCIN RESISTANT BPSC 31 AND
RP   BPSC 61.
RC   STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX   PubMed=6211592; DOI=10.1007/bf00330787;
RA   Higo K., Itoh T., Osawa S.;
RT   "Alteration of ribosomal protein S5 from two spectinomycin-resistant
RT   mutants of Bacillus subtilis: deletion of one amino acid residue.";
RL   Mol. Gen. Genet. 185:205-206(1982).
RN   [7]
RP   CHARACTERIZATION, AND VARIANTS.
RC   STRAIN=168;
RX   PubMed=11489846; DOI=10.1128/jb.183.17.4958-4963.2001;
RA   Inaoka T., Kasai K., Ochi K.;
RT   "Construction of an in vivo nonsense readthrough assay system and
RT   functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 183:4958-4963(2001).
CC   -!- FUNCTION: With S4 and S12 plays an important role in translational
CC       accuracy; many suppressors of streptomycin-dependent mutants of protein
CC       S12 are found in this protein, some but not all of which decrease
CC       translational accuracy (ram, ribosomal ambiguity mutations).
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S4 and S8
CC       (By similarity). {ECO:0000250}.
CC   -!- DOMAIN: The N-terminal domain interacts with the head of the 30S
CC       subunit; the C-terminal domain interacts with the body and contacts
CC       protein S4. The interaction surface between S4 and S5 is involved in
CC       control of translational fidelity.
CC   -!- MISCELLANEOUS: Altered S5 proteins have been identified in a number of
CC       mutants. Some mutations in S5 have been shown to increase translational
CC       error frequencies.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS5 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB59115.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; L47971; AAB06816.1; -; Genomic_DNA.
DR   EMBL; M31102; AAB59115.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL009126; CAB11909.1; -; Genomic_DNA.
DR   EMBL; D64125; BAA20856.1; -; Genomic_DNA.
DR   PIR; D69699; R3BS5S.
DR   RefSeq; NP_388014.1; NC_000964.3.
DR   RefSeq; WP_003328273.1; NZ_JNCM01000029.1.
DR   PDB; 3J9W; EM; 3.90 A; AE=1-166.
DR   PDB; 5NJT; EM; 3.80 A; E=2-166.
DR   PDB; 6HA1; EM; 3.10 A; e=1-166.
DR   PDB; 6HA8; EM; 3.50 A; e=1-166.
DR   PDBsum; 3J9W; -.
DR   PDBsum; 5NJT; -.
DR   PDBsum; 6HA1; -.
DR   PDBsum; 6HA8; -.
DR   SMR; P21467; -.
DR   STRING; 224308.BSU01330; -.
DR   jPOST; P21467; -.
DR   PaxDb; P21467; -.
DR   PRIDE; P21467; -.
DR   EnsemblBacteria; CAB11909; CAB11909; BSU01330.
DR   GeneID; 33964713; -.
DR   GeneID; 938012; -.
DR   KEGG; bsu:BSU01330; -.
DR   PATRIC; fig|224308.179.peg.136; -.
DR   eggNOG; ENOG4108RA9; Bacteria.
DR   eggNOG; COG0098; LUCA.
DR   HOGENOM; HOG000072595; -.
DR   InParanoid; P21467; -.
DR   KO; K02988; -.
DR   OMA; KRGCGSW; -.
DR   PhylomeDB; P21467; -.
DR   BioCyc; BSUB:BSU01330-MONOMER; -.
DR   PRO; PR:P21467; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.230.10; -; 1.
DR   HAMAP; MF_01307_B; Ribosomal_S5_B; 1.
DR   InterPro; IPR000851; Ribosomal_S5.
DR   InterPro; IPR005712; Ribosomal_S5_bac-type.
DR   InterPro; IPR005324; Ribosomal_S5_C.
DR   InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR   InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR   InterPro; IPR013810; Ribosomal_S5_N.
DR   InterPro; IPR018192; Ribosomal_S5_N_CS.
DR   PANTHER; PTHR13718; PTHR13718; 1.
DR   Pfam; PF00333; Ribosomal_S5; 1.
DR   Pfam; PF03719; Ribosomal_S5_C; 1.
DR   SUPFAM; SSF54211; SSF54211; 1.
DR   TIGRFAMs; TIGR01021; rpsE_bact; 1.
DR   PROSITE; PS00585; RIBOSOMAL_S5; 1.
DR   PROSITE; PS50881; S5_DSRBD; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P21467.
DR   SWISS-2DPAGE; P21467.
KW   3D-structure; Antibiotic resistance; Direct protein sequencing;
KW   Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW   rRNA-binding.
FT   CHAIN           1..166
FT                   /note="30S ribosomal protein S5"
FT                   /id="PRO_0000131470"
FT   DOMAIN          11..74
FT                   /note="S5 DRBM"
FT   VARIANT         24..26
FT                   /note="Missing (in 1A241; spectinomycin resistant)"
FT   VARIANT         26
FT                   /note="K -> I (in BSPC 111; spectinomycin resistant)"
FT   VARIANT         28
FT                   /note="G -> V (in rpsE1; spectinomycin restistant, not a
FT                   ram mutant)"
FT   VARIANT         30
FT                   /note="Missing (in BPSC 31 and BPSC 61; spectinomycin
FT                   resistant)"
FT   VARIANT         104
FT                   /note="G -> R (in rpsE9; suppresses S12 mutation K55D.
FT                   Lethal in the absence of the S12 mutation)"
FT   VARIANT         112
FT                   /note="R -> C (in rpsE8; suppresses S12 mutant K55D. RspE8
FT                   is lethal in the absence of the S12 mutation)"
FT   VARIANT         112
FT                   /note="R -> H (in rpsE7; suppresses S12 mutant K55D. A ram
FT                   mutation)"
SQ   SEQUENCE   166 AA;  17623 MW;  5D96AA53FF892AF4 CRC64;
     MRRIDPSKLE LEERLVTVNR VAKVVKGGRR FRFAALVVVG DKNGHVGFGT GKAQEVPEAI
     RKAVEDAKKN LIEVPMVGTT IPHEIIGRFG AGNILLKPAS EGTGVIAGGP VRAVLELAGV
     ADILSKSLGS NTPINMIRAT LQGLSELKRA EDVAKLRGKS VEELLG
//

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