(data stored in ACNUC7421 zone)

SWISSPROT: ECFT_BACSU

ID   ECFT_BACSU              Reviewed;         265 AA.
AC   P70972;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 3.
DT   11-DEC-2019, entry version 93.
DE   RecName: Full=Energy-coupling factor transporter transmembrane protein EcfT;
DE            Short=ECF transporter T component EcfT;
GN   Name=ecfT; Synonyms=ybaF; OrderedLocusNames=BSU01470;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   SEQUENCE REVISION TO 101 AND 262.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [3]
RP   FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RX   PubMed=18931129; DOI=10.1128/jb.01208-08;
RA   Rodionov D.A., Hebbeln P., Eudes A., ter Beek J., Rodionova I.A.,
RA   Erkens G.B., Slotboom D.J., Gelfand M.S., Osterman A.L., Hanson A.D.,
RA   Eitinger T.;
RT   "A novel class of modular transporters for vitamins in prokaryotes.";
RL   J. Bacteriol. 191:42-51(2009).
CC   -!- FUNCTION: Transmembrane (T) component of an energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates (By similarity). Involved in riboflavin transport.
CC       {ECO:0000250, ECO:0000269|PubMed:18931129}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex composed of 2 membrane-embedded substrate-binding proteins (S
CC       component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC       proteins (T component). May be able to interact with more than 1 S
CC       component at a time (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes riboflavin uptake, cell growth less
CC       inhibited by roseoflavin, a toxic riboflavin analog.
CC       {ECO:0000269|PubMed:18931129}.
CC   -!- SIMILARITY: Belongs to the energy-coupling factor EcfT family.
CC       {ECO:0000305}.
DR   EMBL; AL009126; CAB11923.2; -; Genomic_DNA.
DR   PIR; F69742; F69742.
DR   RefSeq; NP_388028.2; NC_000964.3.
DR   RefSeq; WP_004399656.1; NZ_JNCM01000029.1.
DR   STRING; 224308.BSU01470; -.
DR   PaxDb; P70972; -.
DR   PRIDE; P70972; -.
DR   EnsemblBacteria; CAB11923; CAB11923; BSU01470.
DR   GeneID; 938923; -.
DR   KEGG; bsu:BSU01470; -.
DR   PATRIC; fig|224308.179.peg.151; -.
DR   eggNOG; ENOG4105EK6; Bacteria.
DR   eggNOG; COG0619; LUCA.
DR   HOGENOM; HOG000080138; -.
DR   InParanoid; P70972; -.
DR   KO; K16785; -.
DR   OMA; MKAQMSR; -.
DR   PhylomeDB; P70972; -.
DR   BioCyc; BSUB:BSU01470-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01461; EcfT; 1.
DR   InterPro; IPR003339; ABC/ECF_trnsptr_transmembrane.
DR   InterPro; IPR024919; EcfT.
DR   Pfam; PF02361; CbiQ; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P70972.
DR   SWISS-2DPAGE; P70972.
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..265
FT                   /note="Energy-coupling factor transporter transmembrane
FT                   protein EcfT"
FT                   /id="PRO_0000359933"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   265 AA;  29784 MW;  B2FB68F92ECC2C27 CRC64;
     MMDSMIIGKY VPGTSLVHRL DPRTKLITIF LFVCIVFLAN NVQTYALLGL FTIGVVSLTR
     VPFSFLMKGL KPIIWIVLFT FLLHILMTHE GPIIFQIGFF KVYEGGLVQG IFISLRFVYL
     ILITTLLTLT TTPIEITDGM EQLLNPLKKL KLPVHELALM MSISLRFIPT LMEETDKIMK
     AQMARGVDFT SGPVKERVKA IVPLLVPLFV SAFKRAEELA VAMEARGYQG GEGRTKYRKL
     VWTGKDTSVI VSLIVLAALL FFLRA
//

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