(data stored in ACNUC7421 zone)

SWISSPROT: FEUA_BACSU

ID   FEUA_BACSU              Reviewed;         317 AA.
AC   P40409;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   11-DEC-2019, entry version 131.
DE   RecName: Full=Iron-uptake system-binding protein;
DE   Flags: Precursor;
GN   Name=feuA; OrderedLocusNames=BSU01630;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BD99 / MS94;
RX   PubMed=8011666; DOI=10.1016/0005-2728(94)90131-7;
RA   Quirk P.G., Guffanti A.A., Clejan S., Cheng J., Krulwich T.A.;
RT   "Isolation of Tn917 insertional mutants of Bacillus subtilis that are
RT   resistant to the protonophore carbonyl cyanide m-chlorophenylhydrazone.";
RL   Biochim. Biophys. Acta 1186:27-34(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9274029; DOI=10.1099/00221287-143-8-2763;
RA   Liu H., Haga K., Yasumoto K., Ohashi Y., Yoshikawa H., Takahashi H.;
RT   "Sequence and analysis of a 31 kb segment of the Bacillus subtilis
RT   chromosome in the area of the rrnH and rrnG operons.";
RL   Microbiology 143:2763-2767(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, POSSIBLE SUBUNIT, DISRUPTION PHENOTYPE, AND
RP   INDUCTION.
RC   STRAIN=168;
RX   PubMed=16672620; DOI=10.1128/jb.188.10.3664-3673.2006;
RA   Ollinger J., Song K.-B., Antelmann H., Hecker M., Helmann J.D.;
RT   "Role of the Fur regulon in iron transport in Bacillus subtilis.";
RL   J. Bacteriol. 188:3664-3673(2006).
RN   [5]
RP   INDUCTION.
RC   STRAIN=168 / CU1065;
RX   PubMed=17725565; DOI=10.1111/j.1365-2958.2007.05905.x;
RA   Gaballa A., Helmann J.D.;
RT   "Substrate induction of siderophore transport in Bacillus subtilis mediated
RT   by a novel one-component regulator.";
RL   Mol. Microbiol. 66:164-173(2007).
CC   -!- FUNCTION: Involved in the uptake of iron.
CC       {ECO:0000269|PubMed:16672620}.
CC   -!- FUNCTION: Part of the ABC transporter complex FeuABC/YusV involved in
CC       import of the catecholate siderophores bacillibactin and enterobactin.
CC       {ECO:0000305|PubMed:16672620}.
CC   -!- SUBUNIT: The complex is composed of one ATP-binding protein (YusV), two
CC       transmembrane proteins (FeuB and FeuC) and a solute-binding protein
CC       (FeuA). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:16672620};
CC       Lipid-anchor {ECO:0000305|PubMed:16672620}. Cytoplasm
CC       {ECO:0000269|PubMed:16672620}.
CC   -!- INDUCTION: Repressed by fur. Induced by Btr in iron-limited conditions.
CC       {ECO:0000269|PubMed:16672620, ECO:0000269|PubMed:17725565}.
CC   -!- DISRUPTION PHENOTYPE: Strains lacking this gene show a reduction in
CC       growth stimulation by the catecholate siderophores enterobactin and
CC       bacillibactin. {ECO:0000269|PubMed:16672620}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 8 family.
CC       {ECO:0000305}.
DR   EMBL; L19954; AAA64354.1; -; Genomic_DNA.
DR   EMBL; AB002150; BAA19496.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11939.1; -; Genomic_DNA.
DR   PIR; I39842; I39842.
DR   RefSeq; NP_388044.1; NC_000964.3.
DR   RefSeq; WP_003234978.1; NZ_JNCM01000030.1.
DR   PDB; 2PHZ; X-ray; 2.15 A; A=21-317.
DR   PDB; 2WHY; X-ray; 1.70 A; A=21-317.
DR   PDB; 2WI8; X-ray; 1.55 A; A=21-317.
DR   PDB; 2XUZ; X-ray; 1.90 A; A=21-317.
DR   PDB; 2XV1; X-ray; 2.15 A; A=21-317.
DR   PDBsum; 2PHZ; -.
DR   PDBsum; 2WHY; -.
DR   PDBsum; 2WI8; -.
DR   PDBsum; 2XUZ; -.
DR   PDBsum; 2XV1; -.
DR   SMR; P40409; -.
DR   STRING; 224308.BSU01630; -.
DR   TCDB; 3.A.1.14.15; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P40409; -.
DR   PRIDE; P40409; -.
DR   DNASU; 938891; -.
DR   EnsemblBacteria; CAB11939; CAB11939; BSU01630.
DR   GeneID; 938891; -.
DR   KEGG; bsu:BSU01630; -.
DR   PATRIC; fig|224308.179.peg.169; -.
DR   eggNOG; COG0614; LUCA.
DR   HOGENOM; HOG000095659; -.
DR   InParanoid; P40409; -.
DR   KO; K02016; -.
DR   OMA; TIPVSHI; -.
DR   PhylomeDB; P40409; -.
DR   BioCyc; BSUB:BSU01630-MONOMER; -.
DR   EvolutionaryTrace; P40409; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR   InterPro; IPR002491; ABC_transptr_periplasmic_BD.
DR   Pfam; PF01497; Peripla_BP_2; 1.
DR   PROSITE; PS50983; FE_B12_PBP; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P40409.
DR   SWISS-2DPAGE; P40409.
KW   3D-structure; Cell membrane; Cytoplasm; Ion transport; Iron;
KW   Iron transport; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal; Transport.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000305"
FT   CHAIN           20..317
FT                   /note="Iron-uptake system-binding protein"
FT                   /id="PRO_0000031822"
FT   DOMAIN          57..317
FT                   /note="Fe/B12 periplasmic-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00344"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
FT   STRAND          37..43
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          46..53
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          57..59
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           63..72
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          77..82
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           88..90
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   TURN            91..93
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          98..102
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           108..114
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          117..122
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           127..134
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          139..142
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           146..148
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           149..160
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           163..183
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           186..189
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          193..199
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          202..205
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           213..216
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   TURN            217..220
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           226..230
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          232..236
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           239..245
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          248..254
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           256..258
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           265..271
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           273..276
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           279..282
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          286..288
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   STRAND          297..299
FT                   /evidence="ECO:0000244|PDB:2WI8"
FT   HELIX           300..314
FT                   /evidence="ECO:0000244|PDB:2WI8"
SQ   SEQUENCE   317 AA;  35108 MW;  57193CB61E835D25 CRC64;
     MKKISLTLLI LLLALTAAAC GSKNESTASK ASGTASEKKK IEYLDKTYEV TVPTDKIAIT
     GSVESMEDAK LLDVHPQGAI SFSGKFPDMF KDITDKAEPT GEKMEPNIEK ILEMKPDVIL
     ASTKFPEKTL QKISTAGTTI PVSHISSNWK ENMMLLAQLT GKEKKAKKII ADYEQDLKEI
     KTKINDKAKD SKALVIRIRQ GNIYIYPEQV YFNSTLYGDL GLKAPNEVKA AKAQELSSLE
     KLSEMNPDHI FVQFSDDENA DKPDALKDLE KNPIWKSLKA VKEDHVYVNS VDPLAQGGTA
     WSKVRFLKAA AEKLTQN
//

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