(data stored in SCRATCH zone)

SWISSPROT: SKFF_BACSU

ID   SKFF_BACSU              Reviewed;         447 AA.
AC   O31428; Q7DL61;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   11-DEC-2019, entry version 81.
DE   RecName: Full=Putative bacteriocin-SkfA transport system permease protein SkfF;
GN   Name=skfF {ECO:0000303|PubMed:12817086}; Synonyms=ybdB;
GN   OrderedLocusNames=BSU01960;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT   "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT   Bacillus subtilis chromosome.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 205; 216; 396; 412 AND 447.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   POSSIBLE FUNCTION IN SKFA SYNTHESIS, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / PY79;
RX   PubMed=12817086; DOI=10.1126/science.1086462;
RA   Gonzalez-Pastor J.E., Hobbs E.C., Losick R.;
RT   "Cannibalism by sporulating bacteria.";
RL   Science 301:510-513(2003).
RN   [5]
RP   REPRESSION BY ABRB AND ABH.
RX   PubMed=17720793; DOI=10.1128/jb.01081-07;
RA   Strauch M.A., Bobay B.G., Cavanagh J., Yao F., Wilson A., Le Breton Y.;
RT   "Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.";
RL   J. Bacteriol. 189:7720-7732(2007).
CC   -!- FUNCTION: Probably part of the ABC transporter SkfEF involved in the
CC       export of the bacteriocin SKF. Probably responsible for the
CC       translocation of bacteriocin SkfA across the membrane.
CC       {ECO:0000305|PubMed:12817086}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: By Spo0A (PubMed:12817086) and PhoP, during nutrient
CC       starvation, especially phosphate starvation. Repressed by AbrB during
CC       normal growth when nutrients are plentiful, in association with the
CC       transcriptional repressor Abh. {ECO:0000269|PubMed:12817086,
CC       ECO:0000269|PubMed:17720793}.
CC   -!- DISRUPTION PHENOTYPE: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH
CC       operon is deleted, increased rate of spore formation; a double operon
CC       deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster
CC       (PubMed:12817086). {ECO:0000269|PubMed:12817086}.
CC   -!- MISCELLANEOUS: Accelerated cannibalism by skf- cells is seen on solid
CC       media but not in liquid media. {ECO:0000269|PubMed:12817086}.
DR   EMBL; AB006424; BAA33093.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11990.2; -; Genomic_DNA.
DR   PIR; A69747; A69747.
DR   RefSeq; NP_388078.2; NC_000964.3.
DR   RefSeq; WP_010886391.1; NC_000964.3.
DR   STRING; 224308.BSU01960; -.
DR   TCDB; 3.A.1.128.1; the atp-binding cassette (abc) superfamily.
DR   PaxDb; O31428; -.
DR   PRIDE; O31428; -.
DR   EnsemblBacteria; CAB11990; CAB11990; BSU01960.
DR   GeneID; 938494; -.
DR   KEGG; bsu:BSU01960; -.
DR   PATRIC; fig|224308.43.peg.198; -.
DR   BioCyc; BSUB:BSU01960-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030152; P:bacteriocin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR030920; SkfF.
DR   TIGRFAMs; TIGR04405; SkfF; 1.
PE   1: Evidence at protein level;
DR   PRODOM; O31428.
DR   SWISS-2DPAGE; O31428.
KW   Antibiotic biosynthesis; Bacteriocin biosynthesis; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..447
FT                   /note="Putative bacteriocin-SkfA transport system permease
FT                   protein SkfF"
FT                   /id="PRO_0000312741"
FT   TOPO_DOM        1..3
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        4..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..29
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        51..59
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        81..85
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        105..113
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        135..141
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        161..189
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        209..247
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        248..268
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        269..297
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        298..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        319..341
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..360
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        361..363
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        364..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        383..404
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        424..426
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        427..447
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        205
FT                   /note="M -> I (in Ref. 1; BAA33093)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        216
FT                   /note="M -> I (in Ref. 1; BAA33093)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        396
FT                   /note="L -> LL (in Ref. 1; BAA33093)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        412
FT                   /note="S -> T (in Ref. 1; BAA33093)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        447
FT                   /note="R -> K (in Ref. 1; BAA33093)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   447 AA;  51817 MW;  066BD5A67DAAA48B CRC64;
     MPFLIMLLFV GAIGFQVSFV SRSTTWDMSI AGWVLTGVFI LYTAFGLFSN RLPSQMADII
     WLYGTATSFS KVVYSVLFFS VTWKALLWII SAIFGDVLIV LLSGDHINLL GRSIIFVGLF
     FIAEVWLMSV SCARTVKKMK RVYVLVFLLM LGIYSICLYR FFFLQHSSGI WESIARFISG
     VGLVFDTLSP LYVVVFIGII TVSFMTIAFT SRQVEMKESL VKEAEFWEEF QERQFGSGQI
     IQKPKTTWWG LQGLNGIWSF LWLELLLFKK YLFFHSIHTV MLSGVFYVVI FMYPEWFYLL
     FFLIVSAVML SSYYSGIVRH SQSGTLHLFP GALWKKIIIL ELTNTVWLYI LYCVSITFMA
     VGNLVYWYIY GLGIYIWFMT IRLFAFTHTN RNDIKLSLPQ YYKSFFMALG LSGICLYVIH
     LLTADWYTLV VVVCIGSLSW CLFYRFR
//

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