(data stored in ACNUC7421 zone)

SWISSPROT: YBXI_BACSU

ID   YBXI_BACSU              Reviewed;         267 AA.
AC   P54427; O31439;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   11-DEC-2019, entry version 123.
DE   RecName: Full=Probable beta-lactamase YbxI;
DE            EC=3.5.2.6;
DE   Flags: Precursor;
GN   Name=ybxI; Synonyms=ybdS; OrderedLocusNames=BSU02090;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT   "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT   Bacillus subtilis chromosome.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 129-267.
RC   STRAIN=168 / PY79;
RX   PubMed=9016963; DOI=10.1016/s0378-1119(96)00603-8;
RA   Shcheptov M., Chyu G., Bagyan I., Cutting S.M.;
RT   "Characterization of csgA, a new member of the forespore-expressed sigmaG-
RT   regulon from Bacillus subtilis.";
RL   Gene 184:133-140(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10103};
CC   -!- SIMILARITY: Belongs to the class-D beta-lactamase family.
CC       {ECO:0000305}.
DR   EMBL; AB006424; BAA33106.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12003.1; -; Genomic_DNA.
DR   EMBL; X92859; CAA63445.1; -; Genomic_DNA.
DR   PIR; B69752; B69752.
DR   RefSeq; NP_388091.1; NC_000964.3.
DR   RefSeq; WP_003246349.1; NZ_JNCM01000030.1.
DR   PDB; 5E2F; X-ray; 1.30 A; A/B=1-267.
DR   PDBsum; 5E2F; -.
DR   SMR; P54427; -.
DR   STRING; 224308.BSU02090; -.
DR   PaxDb; P54427; -.
DR   PRIDE; P54427; -.
DR   EnsemblBacteria; CAB12003; CAB12003; BSU02090.
DR   GeneID; 938466; -.
DR   KEGG; bsu:BSU02090; -.
DR   PATRIC; fig|224308.179.peg.215; -.
DR   eggNOG; ENOG4108HWY; Bacteria.
DR   eggNOG; COG2602; LUCA.
DR   HOGENOM; HOG000296772; -.
DR   InParanoid; P54427; -.
DR   KO; K17838; -.
DR   OMA; WNRDHTL; -.
DR   PhylomeDB; P54427; -.
DR   BioCyc; BSUB:BSU02090-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008658; F:penicillin binding; IBA:GO_Central.
DR   GO; GO:0017001; P:antibiotic catabolic process; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IBA:GO_Central.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR002137; Beta-lactam_class-D_AS.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   PROSITE; PS00337; BETA_LACTAMASE_D; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P54427.
DR   SWISS-2DPAGE; P54427.
KW   3D-structure; Antibiotic resistance; Hydrolase; Reference proteome; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..267
FT                   /note="Probable beta-lactamase YbxI"
FT                   /id="PRO_0000017049"
FT   REGION          214..216
FT                   /note="Substrate binding"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        76
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10103"
FT   MOD_RES         79
FT                   /note="N6-carboxylysine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        129..137
FT                   /note="YQALARDIG -> IISISERYR (in Ref. 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        169
FT                   /note="T -> A (in Ref. 3; CAA63445)"
FT                   /evidence="ECO:0000305"
FT   HELIX           34..36
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           40..43
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          48..54
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   TURN            55..58
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          59..64
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           65..68
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           75..78
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           81..88
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           109..111
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           117..122
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           126..136
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           138..147
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   TURN            159..163
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          164..167
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           172..183
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          187..189
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           191..200
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          202..205
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          207..217
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   TURN            219..222
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          225..233
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   STRAND          236..246
FT                   /evidence="ECO:0000244|PDB:5E2F"
FT   HELIX           248..261
FT                   /evidence="ECO:0000244|PDB:5E2F"
SQ   SEQUENCE   267 AA;  30644 MW;  BBE3E698CD46E6ED CRC64;
     MKKWIYVVLV LSIAGIGGFS VHAASSAHEK HLNVSKMNVD DEFKDTDGTF ILHDLQKDQT
     FVYNRKRANQ RQTPQSTFKV VNALIGLQVK AVRDEYDVKR WDGVKREFES WNRDHTLGSA
     MRESAIWYYQ ALARDIGEER MKTWLHTLSY GNEDISGGID QFWLQSSLTI SPLEQETFLE
     KLAKEELPFD KPVMKIVKRM MIQEEGDHYT LYGKTGTRLT DMGLGWFVGF IKTEHGSYVF
     VTNVDDSGTK AKNITVDILK KYGLITS
//

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