(data stored in ACNUC7421 zone)

SWISSPROT: YBFK_BACSU

ID   YBFK_BACSU              Reviewed;         296 AA.
AC   O31452; Q7DL48;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   11-DEC-2019, entry version 111.
DE   RecName: Full=Carboxylesterase YbfK;
DE            EC=3.1.1.1;
GN   Name=ybfK; OrderedLocusNames=BSU02260;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT   "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT   Bacillus subtilis chromosome.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=11389736; DOI=10.1046/j.1432-1327.2001.02238.x;
RA   Droege M.J., Bos R., Quax W.J.;
RT   "Paralogous gene analysis reveals a highly enantioselective 1,2-O-
RT   isopropylideneglycerol caprylate esterase of Bacillus subtilis.";
RL   Eur. J. Biochem. 268:3332-3338(2001).
CC   -!- FUNCTION: Shows carboxylesterase activity in vitro.
CC       {ECO:0000269|PubMed:11389736}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC         Evidence={ECO:0000269|PubMed:11389736};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
DR   EMBL; AB006424; BAA33123.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12020.1; -; Genomic_DNA.
DR   PIR; F69749; F69749.
DR   RefSeq; NP_388108.1; NC_000964.3.
DR   RefSeq; WP_003246261.1; NZ_JNCM01000030.1.
DR   PDB; 4CCY; X-ray; 2.04 A; A/B=1-296.
DR   PDBsum; 4CCY; -.
DR   SMR; O31452; -.
DR   STRING; 224308.BSU02260; -.
DR   ESTHER; bacsu-ybfk; 6_AlphaBeta_hydrolase.
DR   PaxDb; O31452; -.
DR   PRIDE; O31452; -.
DR   EnsemblBacteria; CAB12020; CAB12020; BSU02260.
DR   GeneID; 938434; -.
DR   KEGG; bsu:BSU02260; -.
DR   PATRIC; fig|224308.179.peg.232; -.
DR   eggNOG; ENOG4108WC5; Bacteria.
DR   eggNOG; COG0596; LUCA.
DR   HOGENOM; HOG000091205; -.
DR   InParanoid; O31452; -.
DR   OMA; GSHIVNF; -.
DR   PhylomeDB; O31452; -.
DR   BioCyc; BSUB:BSU02260-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   Pfam; PF12697; Abhydrolase_6; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
DR   PRODOM; O31452.
DR   SWISS-2DPAGE; O31452.
KW   3D-structure; Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..296
FT                   /note="Carboxylesterase YbfK"
FT                   /id="PRO_0000360753"
FT   ACT_SITE        129
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        244
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        273
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   HELIX           12..24
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           25..27
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          33..37
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          42..50
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          56..60
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   TURN            63..65
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           67..70
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           71..80
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          81..86
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          91..94
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          96..99
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           104..118
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          123..128
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           130..141
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           143..145
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          146..153
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          155..159
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           164..170
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   TURN            171..173
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           175..186
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           194..205
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           209..211
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          219..221
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           226..230
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          236..241
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          245..247
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           249..259
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   STRAND          264..268
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           275..278
FT                   /evidence="ECO:0000244|PDB:4CCY"
FT   HELIX           280..291
FT                   /evidence="ECO:0000244|PDB:4CCY"
SQ   SEQUENCE   296 AA;  33071 MW;  7315DE166DDEF3BD CRC64;
     MIQDSMQFAA VESGLRFYQA YDQSLSLWPI ESEAFYVSTR FGKTHIIASG PKDAPSLILL
     HGGLFSSAMW YPNIAAWSSQ FRTYAVDIIG DKNKSIPSAA METRADFAEW MKDVFDSLGL
     ETAHLAGLSL GGSHIVNFLL RAPERVERAV VISPAEAFIS FHPDVYKYAA ELTGARGAES
     YIKWITGDSY DLHPLLQRQI VAGVEWQDEQ RSLKPTENGF PYVFTDQELK SIQVPVLLMF
     GEHEAMYHQQ MAFERASVLV PGIQAEIVKN AGHLLSLEQP EYVNQRVLSF LCGGIK
//

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