(data stored in SCRATCH zone)

SWISSPROT: GLNL_BACSU

ID   GLNL_BACSU              Reviewed;         314 AA.
AC   P40759;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   11-DEC-2019, entry version 130.
DE   RecName: Full=Transcriptional regulatory protein GlnL;
GN   Name=glnL; Synonyms=ycbB, yzgB; OrderedLocusNames=BSU02450;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BD99;
RX   PubMed=8011666; DOI=10.1016/0005-2728(94)90131-7;
RA   Quirk P.G., Guffanti A.A., Clejan S., Cheng J., Krulwich T.A.;
RT   "Isolation of Tn917 insertional mutants of Bacillus subtilis that are
RT   resistant to the protonophore carbonyl cyanide m-chlorophenylhydrazone.";
RL   Biochim. Biophys. Acta 1186:27-34(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7704254; DOI=10.1099/13500872-141-2-269;
RA   Ogawa K., Akagawa E., Nakamura K., Yamane K.;
RT   "Determination of a 21548 bp nucleotide sequence around the 24 degrees
RT   region of the Bacillus subtilis chromosome.";
RL   Microbiology 141:269-275(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION.
RX   PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA   Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA   Fujita Y.;
RT   "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT   regulatory systems.";
RL   J. Bacteriol. 183:7365-7370(2001).
RN   [5]
RP   FUNCTION, AND GENE NAME.
RC   STRAIN=168;
RX   PubMed=15995196; DOI=10.1128/jb.187.14.4813-4821.2005;
RA   Satomura T., Shimura D., Asai K., Sadaie Y., Hirooka K., Fujita Y.;
RT   "Enhancement of glutamine utilization in Bacillus subtilis through the
RT   GlnK-GlnL two-component regulatory system.";
RL   J. Bacteriol. 187:4813-4821(2005).
CC   -!- FUNCTION: Member of the two-component regulatory system GlnL/GlnK that
CC       positively regulates the expression of the glsA-glnT operon in response
CC       to glutamine. GlnL binds the promoter region of glsA-glnT in vitro.
CC       {ECO:0000269|PubMed:11717295, ECO:0000269|PubMed:15995196}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by GlnK. {ECO:0000305}.
CC   -!- MISCELLANEOUS: Disruption of glnL by Tn917 insertion leads to
CC       protonophore-resistance.
DR   EMBL; L22503; AAA64347.1; -; Genomic_DNA.
DR   EMBL; D30808; BAA06466.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12039.1; -; Genomic_DNA.
DR   PIR; I39924; I39924.
DR   RefSeq; NP_388127.1; NC_000964.3.
DR   RefSeq; WP_003234824.1; NZ_JNCM01000030.1.
DR   SMR; P40759; -.
DR   STRING; 224308.BSU02450; -.
DR   PaxDb; P40759; -.
DR   PRIDE; P40759; -.
DR   EnsemblBacteria; CAB12039; CAB12039; BSU02450.
DR   GeneID; 938414; -.
DR   KEGG; bsu:BSU02450; -.
DR   PATRIC; fig|224308.179.peg.252; -.
DR   eggNOG; COG0784; LUCA.
DR   HOGENOM; HOG000087762; -.
DR   KO; K07719; -.
DR   OMA; EKPQMRS; -.
DR   BioCyc; BSUB:BSU02450-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   CDD; cd00156; REC; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR013972; YcbB.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF08664; YcbB; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
DR   PRODOM; P40759.
DR   SWISS-2DPAGE; P40759.
KW   Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..314
FT                   /note="Transcriptional regulatory protein GlnL"
FT                   /id="PRO_0000081104"
FT   DOMAIN          2..118
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   MOD_RES         54
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   314 AA;  35711 MW;  EB9D3D1AB1C0B0E4 CRC64;
     MRFFIADDDR AVRSILRQII EDEDLGEAAG EADDGSQVEG HMLQFKQIDI LLIDLLMPGR
     DGIETIRQIQ NTYSGKIVMI SQVEAKEMVG EAYSLGIEYF IHKPINRIEI VTVLQKVKER
     IELEHSIGAI QHSLSRLVNR TERKARPQQK SDSGLKEAGT FLLSELGMMG EGGAHDLMAV
     LQYLAEHEQS EPHEKQSPSL KQIFTQVAVR KLGTGASQTE VNREMKASEQ RIRRAIIHSL
     HHFASLGTTD FSNPKFETYA SKFFDFPVVS QKMKELQSKD AKPLAPARIN MKKFIHVFFL
     EAKLLHETMK QRRI
//

If you have problems or comments...

PBIL Back to PBIL home page