(data stored in ACNUC7421 zone)

SWISSPROT: YCBM_BACSU

ID   YCBM_BACSU              Reviewed;         311 AA.
AC   P42245;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   11-DEC-2019, entry version 123.
DE   RecName: Full=Sensor histidine kinase YcbM;
DE            EC=2.7.13.3;
GN   Name=ycbM; OrderedLocusNames=BSU02560;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7704254; DOI=10.1099/13500872-141-2-269;
RA   Ogawa K., Akagawa E., Nakamura K., Yamane K.;
RT   "Determination of a 21548 bp nucleotide sequence around the 24 degrees
RT   region of the Bacillus subtilis chromosome.";
RL   Microbiology 141:269-275(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   FUNCTION.
RX   PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA   Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA   Fujita Y.;
RT   "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT   regulatory systems.";
RL   J. Bacteriol. 183:7365-7370(2001).
CC   -!- FUNCTION: Member of the two-component regulatory system YcbM/YcbL.
CC       Probably activates YcbL by phosphorylation.
CC       {ECO:0000269|PubMed:11717295}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
DR   EMBL; D30808; BAA06477.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12050.2; -; Genomic_DNA.
DR   PIR; G69753; G69753.
DR   RefSeq; NP_388138.2; NC_000964.3.
DR   RefSeq; WP_003246497.1; NZ_JNCM01000030.1.
DR   SMR; P42245; -.
DR   STRING; 224308.BSU02560; -.
DR   PaxDb; P42245; -.
DR   PRIDE; P42245; -.
DR   EnsemblBacteria; CAB12050; CAB12050; BSU02560.
DR   GeneID; 938397; -.
DR   KEGG; bsu:BSU02560; -.
DR   PATRIC; fig|224308.179.peg.264; -.
DR   HOGENOM; HOG000082595; -.
DR   InParanoid; P42245; -.
DR   OMA; WDRGKGI; -.
DR   PhylomeDB; P42245; -.
DR   BioCyc; BSUB:BSU02560-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IBA:GO_Central.
DR   GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR   GO; GO:0007234; P:osmosensory signaling via phosphorelay pathway; IBA:GO_Central.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
DR   PRODOM; P42245.
DR   SWISS-2DPAGE; P42245.
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..311
FT                   /note="Sensor histidine kinase YcbM"
FT                   /id="PRO_0000074919"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        22..311
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          92..310
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         95
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   CONFLICT        76..228
FT                   /note="Missing (in Ref. 1; BAA06477)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        229..230
FT                   /note="SY -> RI (in Ref. 1; BAA06477)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        234
FT                   /note="N -> L (in Ref. 1; BAA06477)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   311 AA;  35281 MW;  E0A4876A99FD1D1D CRC64;
     MTVLWVAAVI ALACLNVIQF IMKKKRDGNL AYTADQLSYM LSRDSAGQIL LPTDDHALKD
     LLVNINLIVE NRQQISAQFA KTEQSMKRML TNMSHDLKTP LTVILGYIEA IQSDPNMPDE
     ERERLLGKLR QKTNELIQMI NSFFDLAKLE SEDKEIPITK VHMNDICKRN ILHYYDAVQS
     KGFQAAIDIP DTPVYAQANE EALDRILQNL LSNAIQYGAA GKLIGLTLSY DERNIAITVW
     DRGKGISETD QQRVFERLYT LEESRNKAFQ GSGLGLTITK RLTEKMGGII SVQSKPYERT
     AFTITLKRMT Y
//

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