(data stored in SCRATCH zone)

SWISSPROT: TATCD_BACSU

ID   TATCD_BACSU             Reviewed;         242 AA.
AC   P42252; O31468;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 4.
DT   11-DEC-2019, entry version 127.
DE   RecName: Full=Sec-independent protein translocase protein TatCd {ECO:0000255|HAMAP-Rule:MF_00902};
GN   Name=tatC1 {ECO:0000255|HAMAP-Rule:MF_00902}; Synonyms=tatCd, ycbT;
GN   OrderedLocusNames=BSU02640;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7704254; DOI=10.1099/13500872-141-2-269;
RA   Ogawa K., Akagawa E., Nakamura K., Yamane K.;
RT   "Determination of a 21548 bp nucleotide sequence around the 24 degrees
RT   region of the Bacillus subtilis chromosome.";
RL   Microbiology 141:269-275(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 101 AND 104.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 187-242.
RC   STRAIN=168;
RX   PubMed=1353026; DOI=10.1016/0014-5793(92)80656-2;
RA   Awade A., Cleuziat P., Gonzales T., Robert-Baudouy J.;
RT   "Characterization of the pcp gene encoding the pyrrolidone carboxyl
RT   peptidase of Bacillus subtilis.";
RL   FEBS Lett. 305:67-73(1992).
RN   [5]
RP   IDENTIFICATION OF THE TAT GENES, AND SECRETION OF PHOD.
RX   PubMed=11007775; DOI=10.1074/jbc.m004887200;
RA   Jongbloed J.D.H., Martin U., Antelmann H., Hecker M., Tjalsma H.,
RA   Venema G., Bron S., van Dijl J.M., Mueller J.;
RT   "TatC is a specificity determinant for protein secretion via the twin-
RT   arginine translocation pathway.";
RL   J. Biol. Chem. 275:41350-41357(2000).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=12867413; DOI=10.1074/jbc.m306516200;
RA   Pop O.I., Westermann M., Volkmer-Engert R., Schulz D., Lemke C.,
RA   Schreiber S., Gerlach R., Wetzker R., Mueller J.P.;
RT   "Sequence-specific binding of prePhoD to soluble TatAd indicates protein-
RT   mediated targeting of the Tat export in Bacillus subtilis.";
RL   J. Biol. Chem. 278:38428-38436(2003).
RN   [7]
RP   CHARACTERIZATION OF THE TWO TAT TRANSLOCASE SYSTEMS.
RX   PubMed=15554971; DOI=10.1111/j.1365-2958.2004.04341.x;
RA   Jongbloed J.D.H., Grieger U., Antelmann H., Hecker M., Nijland R., Bron S.,
RA   van Dijl J.M.;
RT   "Two minimal Tat translocases in Bacillus.";
RL   Mol. Microbiol. 54:1319-1325(2004).
RN   [8]
RP   INTERACTION WITH TATAD, AND DISRUPTION PHENOTYPE.
RX   PubMed=16698798; DOI=10.1074/jbc.m513900200;
RA   Schreiber S., Stengel R., Westermann M., Volkmer-Engert R., Pop O.I.,
RA   Mueller J.P.;
RT   "Affinity of TatCd for TatAd elucidates its receptor function in the
RT   Bacillus subtilis twin arginine translocation (Tat) translocase system.";
RL   J. Biol. Chem. 281:19977-19984(2006).
RN   [9]
RP   FUNCTION, AND SUBUNIT.
RC   STRAIN=168;
RX   PubMed=18029357; DOI=10.1074/jbc.m708134200;
RA   Barnett J.P., Eijlander R.T., Kuipers O.P., Robinson C.;
RT   "A minimal Tat system from a gram-positive organism: a bifunctional TatA
RT   subunit participates in discrete TatAC and TatA complexes.";
RL   J. Biol. Chem. 283:2534-2542(2008).
CC   -!- FUNCTION: Part of the twin-arginine translocation (Tat) system that
CC       transports large folded proteins containing a characteristic twin-
CC       arginine motif in their signal peptide across membranes. Required for
CC       PhoD secretion. TatCd promotes membrane localization of TatAd via
CC       domain specific interactions. TatCd is required for stabile production
CC       of TatAd as well as for its maintenance. {ECO:0000255|HAMAP-
CC       Rule:MF_00902, ECO:0000269|PubMed:18029357}.
CC   -!- SUBUNIT: Forms a complex with TatAd. Two types of complexes exist: one
CC       composed of TatAd and TatCd, and another composed only of TatAd.
CC       {ECO:0000255|HAMAP-Rule:MF_00902, ECO:0000269|PubMed:18029357}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00902,
CC       ECO:0000269|PubMed:12867413}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_00902, ECO:0000269|PubMed:12867413}.
CC   -!- INDUCTION: Expressed under conditions of phosphate starvation.
CC   -!- DISRUPTION PHENOTYPE: Depletion of TatCd results in a drastic reduction
CC       of TatAd. {ECO:0000269|PubMed:16698798}.
CC   -!- MISCELLANEOUS: B.subtilis possesses two minimal, substrate-specific,
CC       Tat translocases: TatAd-TatCd and TatAy-TatCy, each one composed of a
CC       TatA and a TatC protein. TatA is bifunctional and performs the function
CC       of both the TatA and TatB proteins of Gram-negative organisms.
CC   -!- SIMILARITY: Belongs to the TatC family. {ECO:0000255|HAMAP-
CC       Rule:MF_00902}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA06484.1; Type=Frameshift; Evidence={ECO:0000305};
DR   EMBL; D30808; BAA06484.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AL009126; CAB12058.2; -; Genomic_DNA.
DR   EMBL; X66034; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; D69754; D69754.
DR   RefSeq; NP_388146.2; NC_000964.3.
DR   RefSeq; WP_003246495.1; NZ_JNCM01000030.1.
DR   IntAct; P42252; 24.
DR   STRING; 224308.BSU02640; -.
DR   TCDB; 2.A.64.3.1; the twin arginine targeting (tat) family.
DR   PaxDb; P42252; -.
DR   PRIDE; P42252; -.
DR   EnsemblBacteria; CAB12058; CAB12058; BSU02640.
DR   GeneID; 938395; -.
DR   KEGG; bsu:BSU02640; -.
DR   PATRIC; fig|224308.179.peg.274; -.
DR   eggNOG; ENOG4105DCX; Bacteria.
DR   eggNOG; COG0805; LUCA.
DR   HOGENOM; HOG000245380; -.
DR   InParanoid; P42252; -.
DR   KO; K03118; -.
DR   OMA; EMSFLDH; -.
DR   PhylomeDB; P42252; -.
DR   BioCyc; BSUB:BSU02640-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0033281; C:TAT protein transport complex; IBA:GO_Central.
DR   GO; GO:0009977; F:proton motive force dependent protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0065002; P:intracellular protein transmembrane transport; IBA:GO_Central.
DR   GO; GO:0043953; P:protein transport by the Tat complex; IBA:GO_Central.
DR   HAMAP; MF_00902; TatC; 1.
DR   InterPro; IPR019820; Sec-indep_translocase_CS.
DR   InterPro; IPR002033; TatC.
DR   PANTHER; PTHR30371; PTHR30371; 1.
DR   Pfam; PF00902; TatC; 1.
DR   PRINTS; PR01840; TATCFAMILY.
DR   TIGRFAMs; TIGR00945; tatC; 1.
DR   PROSITE; PS01218; TATC; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P42252.
DR   SWISS-2DPAGE; P42252.
KW   Cell membrane; Membrane; Protein transport; Reference proteome;
KW   Translocation; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..242
FT                   /note="Sec-independent protein translocase protein TatCd"
FT                   /id="PRO_0000098095"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00902"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00902"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00902"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00902"
FT   TRANSMEM        188..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00902"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00902"
FT   REGION          128..149
FT                   /note="Interaction with TatAd"
FT   REGION          171..187
FT                   /note="Interaction with TatAd"
FT   CONFLICT        101
FT                   /note="L -> I (in Ref. 1; BAA06484)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        104
FT                   /note="I -> IMYI (in Ref. 1; BAA06484)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   242 AA;  27706 MW;  CD80471FB2D12F85 CRC64;
     MDKKETHLIG HLEELRRRII VTLAAFFLFL ITAFLFVQDI YDWLIRDLDG KLAVLGPSEI
     LWVYMMLSGI CAIAASIPVA AYQLWRFVAP ALTKTERKVT LMYIPGLFAL FLAGISFGYF
     VLFPIVLSFL THLSSGHFET MFTADRYFRF MVNLSLPFGF LFEMPLVVMF LTRLGILNPY
     RLAKARKLSY FLLIVVSILI TPPDFISDFL VMIPLLVLFE VSVTLSAFVY KKRMREETAA
     AA
//

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