(data stored in ACNUC7421 zone)

SWISSPROT: LMRB_BACSU

ID   LMRB_BACSU              Reviewed;         479 AA.
AC   O35018;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   11-DEC-2019, entry version 115.
DE   RecName: Full=Lincomycin resistance protein LmrB;
GN   Name=lmrB; Synonyms=yccA; OrderedLocusNames=BSU02670;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA   Kumano M., Tamakoshi A., Yamane K.;
RT   "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT   gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT   identification of the site of the lin-2 mutation.";
RL   Microbiology 143:2775-2782(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Proton-dependent transporter. May mediate the efflux of
CC       lincomycin.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC       {ECO:0000305}.
DR   EMBL; AB000617; BAA22228.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12061.1; -; Genomic_DNA.
DR   PIR; E69652; E69652.
DR   RefSeq; NP_388149.1; NC_000964.3.
DR   RefSeq; WP_010886396.1; NZ_JNCM01000030.1.
DR   STRING; 224308.BSU02670; -.
DR   TCDB; 2.A.1.3.30; the major facilitator superfamily (mfs).
DR   PaxDb; O35018; -.
DR   PRIDE; O35018; -.
DR   EnsemblBacteria; CAB12061; CAB12061; BSU02670.
DR   GeneID; 938388; -.
DR   KEGG; bsu:BSU02670; -.
DR   PATRIC; fig|224308.179.peg.277; -.
DR   eggNOG; ENOG4105C0R; Bacteria.
DR   eggNOG; ENOG410XNN3; LUCA.
DR   HOGENOM; HOG000239123; -.
DR   InParanoid; O35018; -.
DR   KO; K18926; -.
DR   OMA; HWIFWIS; -.
DR   PhylomeDB; O35018; -.
DR   BioCyc; BSUB:BSU02670-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR004638; EmrB-like.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00711; efflux_EmrB; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
DR   PRODOM; O35018.
DR   SWISS-2DPAGE; O35018.
KW   Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..479
FT                   /note="Lincomycin resistance protein LmrB"
FT                   /id="PRO_0000173328"
FT   TRANSMEM        19..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        112..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..251
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        272..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        338..355
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        360..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        403..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        449..471
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   479 AA;  51709 MW;  0905DA76EF3A053D CRC64;
     MILETTAKAS QQYKVMPIMI SLLLAGFIGM FSETALNIAL TDLMKELNIT AATVQWLTTG
     YLLVLGILVP VSGLLLQWFT TRQLFTVSLI FSILGTFIAA LAPSFSFLLA ARIVQALGTG
     LLLPLMFNTI LVIFPPHKRG AAMGTIGLVI MFAPAIGPTF SGLVLEHLNW HWIFWISLPF
     LVLALVFGIA YMQNVSETTK PKIDVLSIIL STIGFGGIVF GFSNAGEGSG GWSSPTVIVS
     LIVGVVGLIL FSIRQLTMKQ PMMNLRAFKY PMFILGVIMV FICMMVILSS MLLLPMYLQG
     GLVLTAFASG LVLLPGGILN GFMSPVTGRL FDKYGPKWLV IPGFVIVTVV LWFFSNVTTT
     STAVLIIILH TCLMIGISMI MMPAQTNGLN QLPREFYPDG TAIMNTLQQM AGAIGTAVAV
     SIMAAGQHDY MSTVKNPADP AVIPQALTAG VQHAFVFAMI VAIIGLIGAF FMKRVKVDH
//

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