(data stored in ACNUC7421 zone)

SWISSPROT: NATK_BACSU

ID   NATK_BACSU              Reviewed;         318 AA.
AC   P70954; O34965;
DT   10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2003, sequence version 2.
DT   11-DEC-2019, entry version 118.
DE   RecName: Full=Sensor histidine kinase NatK {ECO:0000305};
DE            EC=2.7.13.3 {ECO:0000250|UniProtKB:P0AEC8};
GN   Name=natK {ECO:0000303|PubMed:17322186};
GN   Synonyms=yccG {ECO:0000312|EMBL:BAA22234.1};
GN   OrderedLocusNames=BSU02730 {ECO:0000312|EMBL:CAB12067.1};
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BD99;
RX   PubMed=9106203; DOI=10.1046/j.1365-2958.1997.2951656.x;
RA   Cheng J., Guffanti A.A., Krulwich T.A.;
RT   "A two-gene ABC-type transport system that extrudes Na+ in Bacillus
RT   subtilis is induced by ethanol or protonophore.";
RL   Mol. Microbiol. 23:1107-1120(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA   Kumano M., Tamakoshi A., Yamane K.;
RT   "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT   gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT   identification of the site of the lin-2 mutation.";
RL   Microbiology 143:2775-2782(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION.
RX   PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA   Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA   Fujita Y.;
RT   "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT   regulatory systems.";
RL   J. Bacteriol. 183:7365-7370(2001).
RN   [5]
RP   FUNCTION.
RC   STRAIN=168;
RX   PubMed=17322186; DOI=10.1099/mic.0.2006/003673-0;
RA   Ogura M., Tsukahara K., Hayashi K., Tanaka T.;
RT   "The Bacillus subtilis NatK-NatR two-component system regulates expression
RT   of the natAB operon encoding an ABC transporter for sodium ion extrusion.";
RL   Microbiology 153:667-675(2007).
CC   -!- FUNCTION: Member of the two-component regulatory system NatK/NatR that
CC       positively regulates the expression of the natAB operon. Potentially
CC       phosphorylates NatR. {ECO:0000269|PubMed:11717295,
CC       ECO:0000269|PubMed:17322186}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3; Evidence={ECO:0000250|UniProtKB:P0AEC8};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
DR   EMBL; U30873; AAB53020.1; -; Genomic_DNA.
DR   EMBL; AB000617; BAA22234.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12067.1; -; Genomic_DNA.
DR   PIR; H69754; H69754.
DR   RefSeq; NP_388155.1; NC_000964.3.
DR   RefSeq; WP_003246454.1; NZ_JNCM01000030.1.
DR   SMR; P70954; -.
DR   STRING; 224308.BSU02730; -.
DR   PaxDb; P70954; -.
DR   PRIDE; P70954; -.
DR   DNASU; 938382; -.
DR   EnsemblBacteria; CAB12067; CAB12067; BSU02730.
DR   GeneID; 938382; -.
DR   KEGG; bsu:BSU02730; -.
DR   PATRIC; fig|224308.179.peg.283; -.
DR   eggNOG; COG3290; LUCA.
DR   HOGENOM; HOG000010994; -.
DR   KO; K11640; -.
DR   OMA; FYISVTN; -.
DR   PhylomeDB; P70954; -.
DR   BioCyc; BSUB:BSU02730-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004673; F:protein histidine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR032834; NatK_C.
DR   Pfam; PF14501; HATPase_c_5; 1.
DR   SMART; SM00387; HATPase_c; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
PE   3: Inferred from homology;
DR   PRODOM; P70954.
DR   SWISS-2DPAGE; P70954.
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..318
FT                   /note="Sensor histidine kinase NatK"
FT                   /id="PRO_0000074920"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          132..318
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000305"
FT   MOD_RES         134
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        164
FT                   /note="S -> N (in Ref. 1; AAB53020)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   318 AA;  35076 MW;  825FFFF993276254 CRC64;
     MITLFQCLYL ILFSFICYQG AAAFSHSTAA SWLAAALGAA AAGLYIWNTK RVWKHCSSGL
     CAWIAVIQVM SVGVVLIGTD IMPVLCVIAI FAGCEGLRIG QSALQARLSD QIDKLTQAEQ
     HANQMLIDVR SRNHDTMKHI TAIKSAQPKA DTQAYIQNWA DQYSQYDRFL KGENAYVAGV
     LYDFLEKARA SNVSVSLHMH TPLSSLPFSP ADQVSLVGNI LENALDSAAE AREKAEIKLE
     TSLRSGLYVL TCENSTPGMD PKVLDTIYQS FGRSTKNGAH EGMGTYIIQK LVKGAFGRLD
     FTYRHPIFRL EIKIPFQK
//

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