(data stored in SCRATCH zone)

SWISSPROT: NATA_BACSU

ID   NATA_BACSU              Reviewed;         246 AA.
AC   P46903;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   11-DEC-2019, entry version 121.
DE   RecName: Full=ABC transporter ATP-binding protein NatA {ECO:0000305};
DE   AltName: Full=ABC-type Na(+) transporter;
DE            EC=7.2.2.4 {ECO:0000269|PubMed:9106203};
GN   Name=natA {ECO:0000303|PubMed:9106203};
GN   OrderedLocusNames=BSU02750 {ECO:0000312|EMBL:CAB12069.1};
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT,
RP   AND INDUCTION.
RC   STRAIN=BD99;
RX   PubMed=9106203; DOI=10.1046/j.1365-2958.1997.2951656.x;
RA   Cheng J., Guffanti A.A., Krulwich T.A.;
RT   "A two-gene ABC-type transport system that extrudes Na+ in Bacillus
RT   subtilis is induced by ethanol or protonophore.";
RL   Mol. Microbiol. 23:1107-1120(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA   Kumano M., Tamakoshi A., Yamane K.;
RT   "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT   gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT   identification of the site of the lin-2 mutation.";
RL   Microbiology 143:2775-2782(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   INDUCTION.
RC   STRAIN=168;
RX   PubMed=17322186; DOI=10.1099/mic.0.2006/003673-0;
RA   Ogura M., Tsukahara K., Hayashi K., Tanaka T.;
RT   "The Bacillus subtilis NatK-NatR two-component system regulates expression
RT   of the natAB operon encoding an ABC transporter for sodium ion extrusion.";
RL   Microbiology 153:667-675(2007).
CC   -!- FUNCTION: Part of an ABC transporter that catalyzes ATP-dependent
CC       electrogenic sodium extrusion. {ECO:0000269|PubMed:9106203}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Na(+)(in) = ADP + H(+) + Na(+)(out) + phosphate;
CC         Xref=Rhea:RHEA:14633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29101, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=7.2.2.4;
CC         Evidence={ECO:0000269|PubMed:9106203};
CC   -!- SUBUNIT: The complex is composed of NatA and NatB.
CC       {ECO:0000269|PubMed:9106203}.
CC   -!- INDUCTION: Induced by ethanol and the protonophore carbonylcyanide p-
CC       chlorophenylhydrazone (CCCP) and, more modestly, by sodium and
CC       potassium. Positively regulated by the two-component regulatory system
CC       NatK/NatR. {ECO:0000269|PubMed:17322186, ECO:0000269|PubMed:9106203}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
DR   EMBL; U30873; AAB53022.1; -; Genomic_DNA.
DR   EMBL; AB000617; BAA22236.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12069.1; -; Genomic_DNA.
DR   PIR; A69666; A69666.
DR   RefSeq; NP_388157.1; NC_000964.3.
DR   RefSeq; WP_003234757.1; NZ_JNCM01000030.1.
DR   SMR; P46903; -.
DR   STRING; 224308.BSU02750; -.
DR   TCDB; 3.A.1.115.1; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P46903; -.
DR   PRIDE; P46903; -.
DR   EnsemblBacteria; CAB12069; CAB12069; BSU02750.
DR   GeneID; 938386; -.
DR   KEGG; bsu:BSU02750; -.
DR   PATRIC; fig|224308.179.peg.285; -.
DR   eggNOG; ENOG4108IUW; Bacteria.
DR   eggNOG; COG4555; LUCA.
DR   InParanoid; P46903; -.
DR   KO; K09697; -.
DR   OMA; YIFMSKL; -.
DR   PhylomeDB; P46903; -.
DR   BioCyc; BSUB:BSU02750-MONOMER; -.
DR   BioCyc; MetaCyc:BSU02750-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATPase activity; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like.
DR   InterPro; IPR017871; ABC_transporter_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P46903.
DR   SWISS-2DPAGE; P46903.
KW   ATP-binding; Ion transport; Nucleotide-binding; Reference proteome; Sodium;
KW   Sodium transport; Translocase; Transport.
FT   CHAIN           1..246
FT                   /note="ABC transporter ATP-binding protein NatA"
FT                   /id="PRO_0000092616"
FT   DOMAIN          2..237
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   NP_BIND         38..45
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   246 AA;  27878 MW;  E84D7C9F6A0642B4 CRC64;
     MITLTDCSRR FQDKKKVVKA VRDVSLTIEK GEVVGILGEN GAGKTTMLRM IASLLEPSQG
     VITVDGFDTV KQPAEVKQRI GVLFGGETGL YDRMTAKENL QYFGRLYGLN RHEIKARIED
     LSKRFGMRDY MNRRVGGFSK GMRQKVAIAR ALIHDPDIIL FDEPTTGLDI TSSNIFREFI
     QQLKREQKTI LFSSHIMEEV QALCDSVIMI HSGEVIYRGA LESLYESERS EDLNYIFMSK
     LVRGIS
//

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