(data stored in ACNUC7421 zone)

SWISSPROT: NATB_BACSU

ID   NATB_BACSU              Reviewed;         386 AA.
AC   P46904;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   11-DEC-2019, entry version 111.
DE   RecName: Full=ABC transporter permease protein NatB {ECO:0000305};
DE   AltName: Full=ABC-type Na(+) transporter;
DE            EC=7.2.2.4 {ECO:0000269|PubMed:9106203};
GN   Name=natB {ECO:0000303|PubMed:9106203};
GN   OrderedLocusNames=BSU02760 {ECO:0000312|EMBL:CAB12070.1};
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT,
RP   AND INDUCTION.
RC   STRAIN=BD99;
RX   PubMed=9106203; DOI=10.1046/j.1365-2958.1997.2951656.x;
RA   Cheng J., Guffanti A.A., Krulwich T.A.;
RT   "A two-gene ABC-type transport system that extrudes Na+ in Bacillus
RT   subtilis is induced by ethanol or protonophore.";
RL   Mol. Microbiol. 23:1107-1120(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDUCTION.
RC   STRAIN=168;
RX   PubMed=17322186; DOI=10.1099/mic.0.2006/003673-0;
RA   Ogura M., Tsukahara K., Hayashi K., Tanaka T.;
RT   "The Bacillus subtilis NatK-NatR two-component system regulates expression
RT   of the natAB operon encoding an ABC transporter for sodium ion extrusion.";
RL   Microbiology 153:667-675(2007).
CC   -!- FUNCTION: Part of an ABC transporter that catalyzes ATP-dependent
CC       electrogenic sodium extrusion. {ECO:0000269|PubMed:9106203}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Na(+)(in) = ADP + H(+) + Na(+)(out) + phosphate;
CC         Xref=Rhea:RHEA:14633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29101, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=7.2.2.4;
CC         Evidence={ECO:0000269|PubMed:9106203};
CC   -!- SUBUNIT: The complex is composed of NatA and NatB.
CC       {ECO:0000269|PubMed:9106203}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Induced by ethanol and the protonophore carbonylcyanide p-
CC       chlorophenylhydrazone (CCCP) and, more modestly, by sodium and
CC       potassium. Positively regulated by the two-component regulatory system
CC       NatK/NatR. {ECO:0000269|PubMed:17322186, ECO:0000269|PubMed:9106203}.
DR   EMBL; U30873; AAB53023.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12070.1; -; Genomic_DNA.
DR   PIR; B69666; B69666.
DR   RefSeq; NP_388158.1; NC_000964.3.
DR   RefSeq; WP_003246346.1; NZ_JNCM01000030.1.
DR   SMR; P46904; -.
DR   IntAct; P46904; 1.
DR   STRING; 224308.BSU02760; -.
DR   TCDB; 3.A.1.115.1; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P46904; -.
DR   PRIDE; P46904; -.
DR   EnsemblBacteria; CAB12070; CAB12070; BSU02760.
DR   GeneID; 938383; -.
DR   KEGG; bsu:BSU02760; -.
DR   PATRIC; fig|224308.179.peg.286; -.
DR   eggNOG; ENOG4108SDH; Bacteria.
DR   eggNOG; COG1668; LUCA.
DR   HOGENOM; HOG000010995; -.
DR   InParanoid; P46904; -.
DR   KO; K09696; -.
DR   OMA; KEAQSYM; -.
DR   PhylomeDB; P46904; -.
DR   BioCyc; BSUB:BSU02760-MONOMER; -.
DR   BioCyc; MetaCyc:BSU02760-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR032688; ABC2_membrane_2.
DR   Pfam; PF12679; ABC2_membrane_2; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P46904.
DR   SWISS-2DPAGE; P46904.
KW   Cell membrane; Ion transport; Membrane; Reference proteome; Sodium;
KW   Sodium transport; Translocase; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..386
FT                   /note="ABC transporter permease protein NatB"
FT                   /id="PRO_0000096741"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..293
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        300..320
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   386 AA;  42793 MW;  B4AA5B2687A478D9 CRC64;
     MLSHIYKKEM IDALRDRKTI LLTILVPMIM MLGLVFFYES MLSDKGEQYT LAVGHSLPPA
     LESKLNEIDE ISVKTFAKPE EAVDEGKADA YLNVPKEFDS YVNSMTPFKV DVYGNSIDQG
     SSNAMQLVQS ALDQYKNEIV QQRLTNKHID QSVIQPFTIQ QKEADEEKGT SAIMLSAILP
     MLILTSIVSG AMPIALDIMA GEKDRKSIEA LLLTPVSRNK VLVGKWLAVS TFGVASGVFA
     LVFLILSTVL FTENLKTAFQ LGDHMWSVIG ASALIIVLSA LLISAMELFI SIMSSSVKEA
     QSYMSLVVFL PVFPMFFIFS KAPNQFDLSY FLIPFLNLHA LFKQLLFGMV DPATILSTSG
     TIAVLIAIFF LLARACFLKD KWVLPK
//

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