(data stored in SCRATCH zone)

SWISSPROT: O16G2_BACSU

ID   O16G2_BACSU             Reviewed;         561 AA.
AC   O34364; Q797R7;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   11-DEC-2019, entry version 117.
DE   RecName: Full=Probable oligo-1,6-glucosidase 2;
DE            EC=3.2.1.10;
DE   AltName: Full=Oligosaccharide alpha-1,6-glucosidase 2;
DE   AltName: Full=Sucrase-isomaltase 2;
DE            Short=Isomaltase 2;
GN   Name=ycdG; OrderedLocusNames=BSU02840;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA   Kumano M., Tamakoshi A., Yamane K.;
RT   "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT   gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT   identification of the site of the lin-2 mutation.";
RL   Microbiology 143:2775-2782(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDUCTION.
RX   PubMed=11544224; DOI=10.1128/jb.183.19.5617-5631.2001;
RA   Petersohn A., Brigulla M., Haas S., Hoheisel J.D., Voelker U., Hecker M.;
RT   "Global analysis of the general stress response of Bacillus subtilis.";
RL   J. Bacteriol. 183:5617-5631(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some
CC         oligosaccharides produced from starch and glycogen by alpha-amylase,
CC         and in isomaltose.; EC=3.2.1.10;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: By ethanol, heat and salt via sigma B-dependent promoter.
CC       {ECO:0000269|PubMed:11544224}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
DR   EMBL; AB000617; BAA22245.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12078.1; -; Genomic_DNA.
DR   PIR; H69755; H69755.
DR   RefSeq; NP_388166.1; NC_000964.3.
DR   RefSeq; WP_003234736.1; NZ_JNCM01000030.1.
DR   SMR; O34364; -.
DR   STRING; 224308.BSU02840; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   PaxDb; O34364; -.
DR   PRIDE; O34364; -.
DR   EnsemblBacteria; CAB12078; CAB12078; BSU02840.
DR   GeneID; 938375; -.
DR   KEGG; bsu:BSU02840; -.
DR   PATRIC; fig|224308.179.peg.295; -.
DR   eggNOG; COG0366; LUCA.
DR   HOGENOM; HOG000220641; -.
DR   InParanoid; O34364; -.
DR   KO; K01182; -.
DR   OMA; YLHMNAV; -.
DR   PhylomeDB; O34364; -.
DR   BioCyc; BSUB:BSU02840-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004556; F:alpha-amylase activity; IBA:GO_Central.
DR   GO; GO:0004574; F:oligo-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009313; P:oligosaccharide catabolic process; IBA:GO_Central.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; O34364.
DR   SWISS-2DPAGE; O34364.
KW   Cytoplasm; Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..561
FT                   /note="Probable oligo-1,6-glucosidase 2"
FT                   /id="PRO_0000360818"
FT   ACT_SITE        199
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        255
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   SITE            330
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   561 AA;  65816 MW;  1E879220CAE04BBF CRC64;
     MKTDWWKDAV VYQIYPRSFQ DSNGDGIGDL RGIISRLDYI KELGADVIWI CPIYPSPNVD
     YGYDVTNHKA IMDSYGTMDD FHELLDQVHQ RGLKLVMDFV LNHTSVEHPW FKEAELDKNS
     KYRSYYYWRP GTKNGPPTDW LSNYGCPVWQ YEEHTGEYYL HMNAVKQADL NWENPEVRQA
     VYDMMKFWLD KGVDGLRIDQ LHLISKKEYL PSYEDYINQQ AEPKPFQPNG ERIHDYLKEI
     TDEVFSHYDV MSVGEVGSVT PEEGLKYTGT DKHELNMIFH FQHMELDQQP GKEHWDLKPL
     ELSDLKSVLT KWQKKLEHQG WNTLFWCNHD QPRIVSRFGD DGEYRKASAK MLAAVIYFMK
     GTPYIYQGEE IGMTNAPFTR IEDYKDIQTI NMYHKRVFEK GYDPNDVMRS ILAKSRDHAR
     TPMQWNSGKN AGFTDGTPWL KVNPNFTAIN VEEAQGDPDS VLNYYKKLIS LRKQYADLMK
     GSFDLLLPDD PQLFVYMREN SKQQLLSVNN FSKEQAVFQW PKNCGKAQAS LLLSNYNNDD
     LDDEMVFRPY ESRVYLLDKT N
//

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