(data stored in SCRATCH zone)

SWISSPROT: BMR3_BACSU

ID   BMR3_BACSU              Reviewed;         512 AA.
AC   P96712; O31471;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   11-DEC-2019, entry version 110.
DE   RecName: Full=Multidrug resistance protein 3;
DE   AltName: Full=Multidrug-efflux transporter 3;
GN   Name=bmr3; Synonyms=mdr; OrderedLocusNames=BSU03070;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A TRANSPORTER, AND
RP   INDUCTION.
RC   STRAIN=168;
RX   PubMed=9023234; DOI=10.1128/jb.179.4.1423-1427.1997;
RA   Ohki R., Murata M.;
RT   "bmr3, a third multidrug transporter gene of Bacillus subtilis.";
RL   J. Bacteriol. 179:1423-1427(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- FUNCTION: Confers resistance to puromycin, tosufloxacin and
CC       norfloxacin. {ECO:0000269|PubMed:9023234}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Expression is dependent on the growth phase and decreases in
CC       the late log phase. {ECO:0000269|PubMed:9023234}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC       {ECO:0000305}.
DR   EMBL; D50098; BAA08793.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12101.2; -; Genomic_DNA.
DR   RefSeq; NP_388189.2; NC_000964.3.
DR   RefSeq; WP_003246377.1; NZ_JNCM01000030.1.
DR   STRING; 224308.BSU03070; -.
DR   TCDB; 2.A.1.3.50; the major facilitator superfamily (mfs).
DR   PRIDE; P96712; -.
DR   EnsemblBacteria; CAB12101; CAB12101; BSU03070.
DR   GeneID; 938345; -.
DR   KEGG; bsu:BSU03070; -.
DR   PATRIC; fig|224308.179.peg.321; -.
DR   HOGENOM; HOG000239126; -.
DR   InParanoid; P96712; -.
DR   OMA; WCILAFG; -.
DR   PhylomeDB; P96712; -.
DR   BioCyc; BSUB:BSU03070-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR004638; EmrB-like.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR001411; Tet-R_TetB/Drug-R_transptr.
DR   PANTHER; PTHR23501; PTHR23501; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00711; efflux_EmrB; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P96712.
DR   SWISS-2DPAGE; P96712.
KW   Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..512
FT                   /note="Multidrug resistance protein 3"
FT                   /id="PRO_0000375916"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        272..292
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        399..421
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        475..495
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   512 AA;  55689 MW;  D789A593F2ADB262 CRC64;
     MDTTTAKQAS TKFVVLGLLL GILMSAMDNT IVATAMGNIV ADLGSFDKFA WVTASYMVAV
     MAGMPIYGKL SDMYGRKRFF LFGLIFFLIG SALCGIAQTM NQLIIFRAIQ GIGGGALLPI
     AFTIIFDLFP PEKRGKMSGM FGAVFGLSSV LGPLLGAIIT DSISWHWVFY INVPIGALSL
     FFIIRYYKES LEHRKQKIDW GGAITLVVSI VCLMFALELG GKTYDWNSIQ IIGLFIVFAV
     FFIAFFIVER KAEEPIISFW MFKNRLFATA QILAFLYGGT FIILAVFIPI FVQAVYGSSA
     TSAGFILTPM MIGSVIGSMI GGIFQTKASF RNLMLISVIA FFIGMLLLSN MTPDTARVWL
     TVFMMISGFG VGFNFSLLPA ASMNDLEPRF RGTANSTNSF LRSFGMTLGV TIFGTVQTNV
     FTNKLNDAFS GMKGSAGSGA AQNIGDPQEI FQAGTRSQIP DAILNRIIDA MSSSITYVFL
     LALIPIVLAA VTILFMGKAR VKTTAEMTKK AN
//

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