(data stored in SCRATCH zone)

SWISSPROT: TMRB_BACSU

ID   TMRB_BACSU              Reviewed;         197 AA.
AC   P12921; P94385;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 4.
DT   11-DEC-2019, entry version 109.
DE   RecName: Full=Tunicamycin resistance protein;
GN   Name=tmrB; OrderedLocusNames=BSU03140;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Harada S., Yoda K., Mori M., Tanimoto A., Furusato T., Yamane K.,
RA   Takatsuki A., Yamasaki M., Tamura G.;
RT   "The gene responsible for tunicamycin resistance, tmrB, in Bacillus
RT   subtilis.";
RL   Agric. Biol. Chem. 52:1785-1789(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   SEQUENCE REVISION TO 33.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [5]
RP   PROTEIN SEQUENCE OF 1-10, AND CHARACTERIZATION.
RX   PubMed=1624425; DOI=10.1128/jb.174.13.4302-4307.1992;
RA   Noda Y., Yoda K., Takatsuki A., Yamasaki M.;
RT   "TmrB protein, responsible for tunicamycin resistance of Bacillus subtilis,
RT   is a novel ATP-binding membrane protein.";
RL   J. Bacteriol. 174:4302-4307(1992).
CC   -!- FUNCTION: Involved in the resistance to tunicamycin. Binds ATP.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA02944.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAA02945.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; D13793; BAA02946.1; -; Genomic_DNA.
DR   EMBL; D13793; BAA02944.1; ALT_INIT; Genomic_DNA.
DR   EMBL; D13793; BAA02945.1; ALT_INIT; Genomic_DNA.
DR   EMBL; D50453; BAA08948.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12108.2; -; Genomic_DNA.
DR   PIR; E69724; YTBST.
DR   RefSeq; NP_388196.2; NC_000964.3.
DR   RefSeq; WP_003246258.1; NZ_JNCM01000030.1.
DR   SMR; P12921; -.
DR   STRING; 224308.BSU03140; -.
DR   PaxDb; P12921; -.
DR   PRIDE; P12921; -.
DR   EnsemblBacteria; CAB12108; CAB12108; BSU03140.
DR   GeneID; 938346; -.
DR   KEGG; bsu:BSU03140; -.
DR   PATRIC; fig|224308.179.peg.328; -.
DR   eggNOG; ENOG4105I1F; Bacteria.
DR   eggNOG; ENOG4111QB4; LUCA.
DR   HOGENOM; HOG000253746; -.
DR   InParanoid; P12921; -.
DR   OMA; AKQIDRC; -.
DR   PhylomeDB; P12921; -.
DR   BioCyc; BSUB:BSU03140-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P12921.
DR   SWISS-2DPAGE; P12921.
KW   Antibiotic resistance; ATP-binding; Cell membrane;
KW   Direct protein sequencing; Membrane; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..197
FT                   /note="Tunicamycin resistance protein"
FT                   /id="PRO_0000072604"
FT   NP_BIND         7..14
FT                   /note="ATP"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        33
FT                   /note="E -> Q (in Ref. 1; BAA02946/BAA02944/BAA02945 and 2;
FT                   BAA08948)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   197 AA;  22730 MW;  FBBB871924642EF2 CRC64;
     MIIWINGAFG SGKTQTAFEL HRRLNPSYVY DPEKMGFALR SMVPQEIAKD DFQSYPLWRA
     FNYSLLASLT DTYRGILIVP MTIVHPEYFN EIIGRLRQEG RIVHHFTLMA SKETLLKRLR
     TRAEGKNSWA AKQIDRCVEG LSSPIFEDHI QTDNLSIQDV AENIAARAEL PLDPDTRGSL
     RRFADRLMVK LNHIRIK
//

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