(data stored in ACNUC7421 zone)

SWISSPROT: PUTP_BACSU

ID   PUTP_BACSU              Reviewed;         473 AA.
AC   P94392; Q797Q4;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 3.
DT   11-DEC-2019, entry version 122.
DE   RecName: Full=High-affinity proline transporter PutP {ECO:0000303|PubMed:22139509};
GN   Name=putP {ECO:0000303|PubMed:22139509}; Synonyms=ycgO;
GN   OrderedLocusNames=BSU03220;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDUCTION.
RX   PubMed=21840319; DOI=10.1016/j.jmb.2011.08.003;
RA   Belitsky B.R.;
RT   "Indirect repression by Bacillus subtilis CodY via displacement of the
RT   activator of the proline utilization operon.";
RL   J. Mol. Biol. 413:321-336(2011).
RN   [4]
RP   INDUCTION.
RC   STRAIN=168;
RX   PubMed=21964733; DOI=10.1099/mic.0.054197-0;
RA   Huang S.C., Lin T.H., Shaw G.C.;
RT   "PrcR, a PucR-type transcriptional activator, is essential for proline
RT   utilization and mediates proline-responsive expression of the proline
RT   utilization operon putBCP in Bacillus subtilis.";
RL   Microbiology 157:3370-3377(2011).
RN   [5]
RP   FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=168 / JH642;
RX   PubMed=22139509; DOI=10.1128/jb.06380-11;
RA   Moses S., Sinner T., Zaprasis A., Stoeveken N., Hoffmann T., Belitsky B.R.,
RA   Sonenshein A.L., Bremer E.;
RT   "Proline utilization by Bacillus subtilis: uptake and catabolism.";
RL   J. Bacteriol. 194:745-758(2012).
CC   -!- FUNCTION: Catalyzes the high-affinity uptake of extracellular proline.
CC       Important for the use of proline as a sole carbon and energy source or
CC       a sole nitrogen source. {ECO:0000269|PubMed:22139509}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=8 uM for proline {ECO:0000269|PubMed:22139509};
CC         Vmax=29 nmol/min/mg enzyme {ECO:0000269|PubMed:22139509};
CC         Vmax=28 nmol/min/mg enzyme (in the presence of 0.4 M NaCl)
CC         {ECO:0000269|PubMed:22139509};
CC         Vmax=158 nmol/min/mg enzyme (in the presence of 1 mM proline)
CC         {ECO:0000269|PubMed:22139509};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: The expression of the putBCP operon is induced in a PutR-
CC       dependent fashion by very low concentrations of L-proline in the growth
CC       medium. CodY represses the operon by displacing PutR from DNA.
CC       {ECO:0000269|PubMed:21840319, ECO:0000269|PubMed:21964733,
CC       ECO:0000269|PubMed:22139509}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the putBCP operon abolishes L-proline
CC       utilization. {ECO:0000269|PubMed:22139509}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA08956.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
DR   EMBL; D50453; BAA08956.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL009126; CAB12116.2; -; Genomic_DNA.
DR   PIR; B69759; B69759.
DR   RefSeq; NP_388204.2; NC_000964.3.
DR   RefSeq; WP_010886399.1; NC_000964.3.
DR   STRING; 224308.BSU03220; -.
DR   PaxDb; P94392; -.
DR   PRIDE; P94392; -.
DR   EnsemblBacteria; CAB12116; CAB12116; BSU03220.
DR   GeneID; 938330; -.
DR   KEGG; bsu:BSU03220; -.
DR   PATRIC; fig|224308.43.peg.330; -.
DR   eggNOG; ENOG4105CK7; Bacteria.
DR   eggNOG; COG0591; LUCA.
DR   HOGENOM; HOG000282935; -.
DR   InParanoid; P94392; -.
DR   KO; K11928; -.
DR   OMA; CWTDLIQ; -.
DR   PhylomeDB; P94392; -.
DR   BioCyc; BSUB:BSU03220-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IBA:GO_Central.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   InterPro; IPR018212; Na/solute_symporter_CS.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS00456; NA_SOLUT_SYMP_1; 1.
DR   PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P94392.
DR   SWISS-2DPAGE; P94392.
KW   Cell membrane; Ion transport; Membrane; Reference proteome; Sodium;
KW   Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..473
FT                   /note="High-affinity proline transporter PutP"
FT                   /id="PRO_0000360821"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        376..396
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        408..428
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        431..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   473 AA;  51144 MW;  71B3A7E237F2D937 CRC64;
     MLLIGYFAYK RTSNLTDYML GGRSLGPAVT ALSAGAADMS GWLLMGLPGA MFSTGLSGAW
     IVIGLCLGAW ANWLYVAPRL RTYTEKAGNS ITIPGFLENR FGDQTKLLRL FSGIVILVFF
     TFYVSSGMVS GGVLFNSILG MDYHTGLWIV TGVVVAYTLF GGFLAVSWTD FVQGIIMFAA
     LILVPIVTFF HTGGAGDTVA EIRSVDPDMF NIFKGTSVLG IISLFAWGLG YFGQPHIIVR
     FMAITSVKEI KRARRIGMGW MILSAVGAVL TGLGGIAYYH QRGMTLKDPE TIFIQLGNIL
     FHPIITGFLI SAILAAIMST ISSQLLVTSS SLVEDLYKSM FRRSASDKEL VFLGRLAVLA
     VSIVALVLAW EKNNTILGLV SYAWAGFGAS FGPVVLLSLF WKRMTKWGAL AGMIVGAATV
     IIWANAGLSD FLYEMIPGFA ASLLSVFFVS ILTQAPSQAV TDQFNDYQDT MSQ
//

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