(data stored in ACNUC7421 zone)

SWISSPROT: TCYC_BACSU

ID   TCYC_BACSU              Reviewed;         247 AA.
AC   P39456;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 4.
DT   11-DEC-2019, entry version 128.
DE   RecName: Full=L-cystine import ATP-binding protein TcyC;
DE            EC=7.4.2.-;
GN   Name=tcyC; Synonyms=yckI; OrderedLocusNames=BSU03590;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=7551042; DOI=10.1099/13500872-141-7-1781;
RA   Rodriguez F., Grandi G.;
RT   "An operon encoding a novel ABC-type transport system in Bacillus
RT   subtilis.";
RL   Microbiology 141:1781-1784(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8407792; DOI=10.1128/jb.175.19.6203-6211.1993;
RA   Grossman T.H., Tuckman M., Ellestad S., Osburne M.S.;
RT   "Isolation and characterization of Bacillus subtilis genes involved in
RT   siderophore biosynthesis: relationship between B. subtilis sfpo and
RT   Escherichia coli entD genes.";
RL   J. Bacteriol. 175:6203-6211(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   SEQUENCE REVISION TO 84.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-247.
RC   STRAIN=168 / JH642;
RX   PubMed=8355609; DOI=10.1111/j.1365-2958.1993.tb01629.x;
RA   Cosmina P., Rodriguez F., de Ferra F., Grandi G., Perego M., Venema G.,
RA   van Sinderen D.;
RT   "Sequence and analysis of the genetic locus responsible for surfactin
RT   synthesis in Bacillus subtilis.";
RL   Mol. Microbiol. 8:821-831(1993).
RN   [7]
RP   FUNCTION IN L-CYSTINE TRANSPORT.
RC   STRAIN=168;
RX   PubMed=15262924; DOI=10.1128/jb.186.15.4875-4884.2004;
RA   Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT   "Three different systems participate in L-cystine uptake in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 186:4875-4884(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex TcyABC involved in L-
CC       cystine import. Responsible for energy coupling to the transport system
CC       (Probable). {ECO:0000305|PubMed:15262924}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (TcyC),
CC       two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. L-cystine
CC       importer (TC 3.A.1.3.14) family. {ECO:0000305}.
DR   EMBL; X77636; CAA54726.1; -; Genomic_DNA.
DR   EMBL; L17438; AAC36828.1; -; Unassigned_DNA.
DR   EMBL; D50453; BAA08992.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12153.2; -; Genomic_DNA.
DR   EMBL; X70356; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; C69761; C69761.
DR   RefSeq; NP_388241.2; NC_000964.3.
DR   RefSeq; WP_003246629.1; NZ_JNCM01000031.1.
DR   SMR; P39456; -.
DR   STRING; 224308.BSU03590; -.
DR   TCDB; 3.A.1.3.14; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P39456; -.
DR   PRIDE; P39456; -.
DR   EnsemblBacteria; CAB12153; CAB12153; BSU03590.
DR   GeneID; 938294; -.
DR   KEGG; bsu:BSU03590; -.
DR   PATRIC; fig|224308.179.peg.378; -.
DR   eggNOG; ENOG4105CDA; Bacteria.
DR   eggNOG; COG1126; LUCA.
DR   InParanoid; P39456; -.
DR   KO; K10010; -.
DR   OMA; FQAYHLF; -.
DR   PhylomeDB; P39456; -.
DR   BioCyc; BSUB:BSU03590-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATPase activity; IEA:InterPro.
DR   GO; GO:0015424; F:ATPase-coupled amino acid transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR030679; ABC_ATPase_HisP-typ.
DR   InterPro; IPR003439; ABC_transporter-like.
DR   InterPro; IPR017871; ABC_transporter_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   PIRSF; PIRSF039085; ABC_ATPase_HisP; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P39456.
DR   SWISS-2DPAGE; P39456.
KW   Amino-acid transport; ATP-binding; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Translocase; Transport.
FT   CHAIN           1..247
FT                   /note="L-cystine import ATP-binding protein TcyC"
FT                   /id="PRO_0000093138"
FT   DOMAIN          2..240
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   NP_BIND         34..41
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   CONFLICT        72..93
FT                   /note="KQADILKLRRKSGMVFQAYHLF -> NRRISKASPKIRNGVSGVSPV (in
FT                   Ref. 2; AAC36828)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        84
FT                   /note="G -> P (in Ref. 3; BAA08992 and 6; X70356)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   247 AA;  27748 MW;  CB9CE075CA33C193 CRC64;
     MLTVKGLNKS FGENEILKKI DMKIEKGKVI AILGPSGSGK TTLLRCLNAL EIPNRGELAF
     DDFSIDFSKK VKQADILKLR RKSGMVFQAY HLFPHRTALE NVMEGPVQVQ KRNKEEVRKE
     AIQLLDKVGL KDKMDLYPFQ LSGGQQQRVG IARALAIQPE LMLFDEPTSA LDPELVGEVL
     KVIKDLANEG WTMVVVTHEI KFAQEVADEV IFIDGGVIVE QGPPEQIFSA PKEERTQRFL
     NRILNPL
//

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