(data stored in ACNUC7421 zone)

SWISSPROT: TCYA_BACSU

ID   TCYA_BACSU              Reviewed;         268 AA.
AC   P42199; P94402;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 3.
DT   11-DEC-2019, entry version 119.
DE   RecName: Full=L-cystine-binding protein TcyA;
DE   Flags: Precursor;
GN   Name=tcyA; Synonyms=yckK; OrderedLocusNames=BSU03610;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=7551042; DOI=10.1099/13500872-141-7-1781;
RA   Rodriguez F., Grandi G.;
RT   "An operon encoding a novel ABC-type transport system in Bacillus
RT   subtilis.";
RL   Microbiology 141:1781-1784(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   SEQUENCE REVISION TO 95 AND 190.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [5]
RP   FUNCTION IN L-CYSTINE TRANSPORT.
RC   STRAIN=168;
RX   PubMed=15262924; DOI=10.1128/jb.186.15.4875-4884.2004;
RA   Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT   "Three different systems participate in L-cystine uptake in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 186:4875-4884(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex TcyABC involved in L-
CC       cystine import. {ECO:0000269|PubMed:15262924}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (TcyC),
CC       two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 3 family.
CC       {ECO:0000305}.
DR   EMBL; X77636; CAA54724.1; -; Genomic_DNA.
DR   EMBL; D50453; BAA08994.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12155.2; -; Genomic_DNA.
DR   PIR; E69761; E69761.
DR   RefSeq; NP_388243.2; NC_000964.3.
DR   RefSeq; WP_003246699.1; NZ_JNCM01000031.1.
DR   SMR; P42199; -.
DR   STRING; 224308.BSU03610; -.
DR   TCDB; 3.A.1.3.14; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P42199; -.
DR   PRIDE; P42199; -.
DR   EnsemblBacteria; CAB12155; CAB12155; BSU03610.
DR   GeneID; 938298; -.
DR   KEGG; bsu:BSU03610; -.
DR   PATRIC; fig|224308.179.peg.380; -.
DR   eggNOG; COG0834; LUCA.
DR   HOGENOM; HOG000031895; -.
DR   InParanoid; P42199; -.
DR   KO; K02424; -.
DR   OMA; KFVPTKW; -.
DR   PhylomeDB; P42199; -.
DR   BioCyc; BSUB:BSU03610-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR018313; SBP_3_CS.
DR   InterPro; IPR001638; Solute-binding_3/MltF_N.
DR   Pfam; PF00497; SBP_bac_3; 1.
DR   SMART; SM00062; PBPb; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR   PROSITE; PS01039; SBP_BACTERIAL_3; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P42199.
DR   SWISS-2DPAGE; P42199.
KW   Amino-acid transport; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal; Transport.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..268
FT                   /note="L-cystine-binding protein TcyA"
FT                   /id="PRO_0000031778"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CONFLICT        81..82
FT                   /note="KR -> NS (in Ref. 1; CAA54724)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        95
FT                   /note="D -> G (in Ref. 2; BAA08994)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        120..121
FT                   /note="DK -> NQ (in Ref. 1; CAA54724)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        190
FT                   /note="G -> A (in Ref. 2; BAA08994)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   268 AA;  29514 MW;  E88B56676E8AD762 CRC64;
     MKKALLALFM VVSIAALAAC GAGNDNQSKD NAKDGDLWAS IKKKGVLTVG TEGTYEPFTY
     HDKDTDKLTG YDVEVITEVA KRLGLKVDFK ETQWDSMFAG LNSKRFDVVA NQVGKTDRED
     KYDFSDKYTT SRAVVVTKKD NNDIKSEADV KGKTSAQSLT SNYNKLATNA GAKVEGVEGM
     AQALQMIQQG RVDMTYNDKL AVLNYLKTSG NKNVKIAFET GEPQSTYFTF RKGSGEVVDQ
     VNKALKEMKE DGTLSKISKK WFGEDVSK
//

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