(data stored in SCRATCH zone)

SWISSPROT: GERKA_BACSU

ID   GERKA_BACSU             Reviewed;         544 AA.
AC   P49939; P94410;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   11-DEC-2019, entry version 99.
DE   RecName: Full=Spore germination protein KA;
GN   Name=gerKA; OrderedLocusNames=BSU03700;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Irie R., Fujita Y.Y.F., Kobayasi M.;
RT   "Nucleotide sequence and gene organization of the gerK spore germinating
RT   locus of Bacillus subtilis 168.";
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Involved in the germination response to the combination of
CC       glucose, fructose, L-asparagine, and KCl.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GerABKA family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA11254.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; D78187; BAA11254.1; ALT_INIT; Genomic_DNA.
DR   EMBL; D50453; BAA09002.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12178.1; -; Genomic_DNA.
DR   PIR; I39858; I39858.
DR   RefSeq; NP_388252.1; NC_000964.3.
DR   RefSeq; WP_003246515.1; NZ_JNCM01000031.1.
DR   STRING; 224308.BSU03700; -.
DR   PaxDb; P49939; -.
DR   PRIDE; P49939; -.
DR   EnsemblBacteria; CAB12178; CAB12178; BSU03700.
DR   GeneID; 938285; -.
DR   KEGG; bsu:BSU03700; -.
DR   PATRIC; fig|224308.179.peg.390; -.
DR   eggNOG; ENOG4105E1K; Bacteria.
DR   eggNOG; ENOG410XNMP; LUCA.
DR   HOGENOM; HOG000059245; -.
DR   InParanoid; P49939; -.
DR   KO; K06295; -.
DR   OMA; SHCITEE; -.
DR   PhylomeDB; P49939; -.
DR   BioCyc; BSUB:BSU03700-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009847; P:spore germination; IEA:InterPro.
DR   InterPro; IPR004995; Spore_Ger.
DR   Pfam; PF03323; GerA; 1.
DR   PIRSF; PIRSF005690; GerBA; 1.
PE   3: Inferred from homology;
DR   PRODOM; P49939.
DR   SWISS-2DPAGE; P49939.
KW   Cell membrane; Germination; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..544
FT                   /note="Spore germination protein KA"
FT                   /id="PRO_0000164016"
FT   TRANSMEM        279..299
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        321..341
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..412
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        416..436
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        2
FT                   /note="P -> R (in Ref. 1; BAA11254)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        108
FT                   /note="Q -> E (in Ref. 1; BAA11254)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        111
FT                   /note="E -> R (in Ref. 1; BAA11254)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        114
FT                   /note="L -> A (in Ref. 1; BAA11254)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        199
FT                   /note="E -> G (in Ref. 1; BAA11254)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        202
FT                   /note="K -> E (in Ref. 1; BAA11254)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        376
FT                   /note="E -> K (in Ref. 1; BAA11254)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   544 AA;  60386 MW;  131CFBBF45EF882A CRC64;
     MPLFSKRKNN TDSKDKQNTD ERNQEQQQEK ERPVLISPSL AKNIAETKKE VGSSSDVIIR
     EIKIGEQDHV HLAVIYISGL VDNNTIHESL IDPLVQDESI QNTHAIQQIL EKTLPLGGVK
     AEKSWDKLFS ELMLGNALIF ADGHDEALIC STQGGEQRSI QEPSTQVSFR GPRQGFTESL
     QTNISMIRRY IKNPNLWVEK MKKGSVTNTD IALMYIQGIC DEKVLKEVKQ RLEKIDIDSI
     LESGYIEQLI EDETFTTFPT MYHTERPDVV AGNLLEGRFA IIVDGTPFVL IAPALFVQFF
     QSVEDYYSRF DIATSIRILR VLVFFISLVA PAVYVAATTF HQEMIPTQLL VVIAAQREIV
     PFPAVVEALT MEVAFEILRE AGVRLPRVVG SAVSIVGALV IGQAAVQAGI VSPAMVIIVA
     LTAIASFATP AFAMAISARL IRFIFIIASA VMGFYGLILG IIMMFVHLCS LRSFGVPYMS
     PLAPFSSQGV KDALFRVPWW ADEKRPESVS KEDKVRQGKD QRPEPAASRG MVNKDLEEGD
     QNGT
//

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