(data stored in ACNUC7421 zone)

SWISSPROT: YCLN_BACSU

ID   YCLN_BACSU              Reviewed;         316 AA.
AC   P94418; Q797P2;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   11-DEC-2019, entry version 103.
DE   RecName: Full=Petrobactin import system permease protein YclN {ECO:0000305};
GN   Name=yclN; OrderedLocusNames=BSU03800;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168;
RX   PubMed=19955416; DOI=10.1073/pnas.0904793106;
RA   Zawadzka A.M., Kim Y., Maltseva N., Nichiporuk R., Fan Y., Joachimiak A.,
RA   Raymond K.N.;
RT   "Characterization of a Bacillus subtilis transporter for petrobactin, an
RT   anthrax stealth siderophore.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:21854-21859(2009).
CC   -!- FUNCTION: Part of the ABC transporter complex YclNOPQ involved in
CC       uptake of ferric-petrobactin. Petrobactin is a photoreactive 3,4-
CC       catecholate siderophore produced by many members of the B.cereus group,
CC       including B.anthracis. Probably responsible for the translocation of
CC       the substrate across the membrane. {ECO:0000269|PubMed:19955416}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (YclP),
CC       two transmembrane proteins (YclN and YclO) and a solute-binding protein
CC       (YclQ). {ECO:0000305|PubMed:19955416}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Disruption mutants are unable to use petrobactin
CC       for iron delivery and growth. {ECO:0000269|PubMed:19955416}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. FecCD subfamily. {ECO:0000305}.
DR   EMBL; D50453; BAA09012.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12188.1; -; Genomic_DNA.
DR   PIR; B69763; B69763.
DR   RefSeq; NP_388262.1; NC_000964.3.
DR   RefSeq; WP_003234491.1; NZ_JNCM01000031.1.
DR   STRING; 224308.BSU03800; -.
DR   PaxDb; P94418; -.
DR   EnsemblBacteria; CAB12188; CAB12188; BSU03800.
DR   GeneID; 938273; -.
DR   KEGG; bsu:BSU03800; -.
DR   PATRIC; fig|224308.179.peg.403; -.
DR   eggNOG; ENOG4105DHS; Bacteria.
DR   eggNOG; COG4606; LUCA.
DR   HOGENOM; HOG000045408; -.
DR   InParanoid; P94418; -.
DR   KO; K02015; -.
DR   OMA; WFAGSFT; -.
DR   PhylomeDB; P94418; -.
DR   BioCyc; BSUB:BSU03800-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005623; C:cell; IEA:GOC.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0033214; P:siderophore-dependent iron import into cell; IBA:GO_Central.
DR   Gene3D; 1.10.3470.10; -; 1.
DR   InterPro; IPR037294; ABC_BtuC-like.
DR   InterPro; IPR000522; ABC_transptr_permease_BtuC.
DR   PANTHER; PTHR30472; PTHR30472; 1.
DR   Pfam; PF01032; FecCD; 1.
DR   SUPFAM; SSF81345; SSF81345; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P94418.
DR   SWISS-2DPAGE; P94418.
KW   Cell membrane; Ion transport; Iron; Iron transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..316
FT                   /note="Petrobactin import system permease protein YclN"
FT                   /id="PRO_0000359514"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..288
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        290..310
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   316 AA;  34899 MW;  70C1C696389C8834 CRC64;
     MKLRYLFILL IILAVTSVFI GVEDLSPLDL FDLSKQEAST LFASRLPRLI SIVIAGLSMS
     ICGLIMQQIS RNKFVSPTTA GTMDWARLGI LISLLLFTSA SPLIKMLVAF VFALAGNFLF
     MKILERIKFN DTIFIPLVGL MLGNIVSSIA TFIAYKYDLI QNVSSWLQGD FSLVVKGRYE
     LLYLSIPLVI IAYVYADKFT LAGMGESFSV NLGLKYKRVV NIGLIIVSLI TSLVILTVGM
     LPFLGLIIPN IVSIYRGDNL KSSLPHTVLL GAVFVLFCDI LGRIIIFPYE ISIGLMVGII
     GSGIFLFMLL RRKAYA
//

If you have problems or comments...

PBIL Back to PBIL home page