(data stored in ACNUC7421 zone)

SWISSPROT: PBPC_BACSU

ID   PBPC_BACSU              Reviewed;         668 AA.
AC   P42971; P40772;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   11-DEC-2019, entry version 130.
DE   RecName: Full=Penicillin-binding protein 3;
DE            Short=PBP 3;
DE   AltName: Full=PSPB20;
DE   AltName: Full=Penicillin-binding protein C;
GN   Name=pbpC; Synonyms=ycsM, yzsA; OrderedLocusNames=BSU04140;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8574415; DOI=10.1099/13500872-141-12-3241;
RA   Akagawa E., Kurita K., Sugawara T., Nakamura K., Kasahara Y., Ogasawara N.,
RA   Yamane K.;
RT   "Determination of a 17,484 bp nucleotide sequence around the 39 degrees
RT   region of the Bacillus subtilis chromosome and similarity analysis of the
RT   products of putative ORFs.";
RL   Microbiology 141:3241-3245(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-37.
RX   PubMed=3145906; DOI=10.1016/0378-1119(88)90207-7;
RA   Smith H., de Jong A., Bron S., Venema G.;
RT   "Characterization of signal-sequence-coding regions selected from the
RT   Bacillus subtilis chromosome.";
RL   Gene 70:351-361(1988).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the transpeptidase family. {ECO:0000305}.
DR   EMBL; D38161; BAA07365.1; -; Genomic_DNA.
DR   EMBL; D50453; BAA09043.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12221.1; -; Genomic_DNA.
DR   EMBL; M22913; AAA22829.1; -; Genomic_DNA.
DR   PIR; I39902; I39902.
DR   RefSeq; NP_388295.1; NC_000964.3.
DR   RefSeq; WP_003246590.1; NZ_JNCM01000031.1.
DR   SMR; P42971; -.
DR   IntAct; P42971; 1.
DR   STRING; 224308.BSU04140; -.
DR   ChEMBL; CHEMBL3112383; -.
DR   DrugBank; DB00355; Aztreonam.
DR   DrugBank; DB00493; Cefotaxime.
DR   DrugBank; DB01598; Imipenem.
DR   DrugBank; DB04570; Latamoxef.
DR   DrugCentral; P42971; -.
DR   jPOST; P42971; -.
DR   PaxDb; P42971; -.
DR   PRIDE; P42971; -.
DR   EnsemblBacteria; CAB12221; CAB12221; BSU04140.
DR   GeneID; 940139; -.
DR   KEGG; bsu:BSU04140; -.
DR   PATRIC; fig|224308.179.peg.440; -.
DR   eggNOG; ENOG4107RVG; Bacteria.
DR   eggNOG; COG0768; LUCA.
DR   HOGENOM; HOG000236144; -.
DR   InParanoid; P42971; -.
DR   KO; K21467; -.
DR   OMA; TLACKTG; -.
DR   PhylomeDB; P42971; -.
DR   BioCyc; BSUB:BSU04140-MONOMER; -.
DR   PRO; PR:P42971; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0008658; F:penicillin binding; IBA:GO_Central.
DR   GO; GO:0071555; P:cell wall organization; IBA:GO_Central.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:InterPro.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR007887; MecA_N.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR005311; PBP_dimer.
DR   InterPro; IPR036138; PBP_dimer_sf.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   Pfam; PF05223; MecA_N; 1.
DR   Pfam; PF03717; PBP_dimer; 1.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
DR   SUPFAM; SSF56519; SSF56519; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
PE   3: Inferred from homology;
DR   PRODOM; P42971.
DR   SWISS-2DPAGE; P42971.
KW   Cell membrane; Cell shape; Cell wall biogenesis/degradation; Membrane;
KW   Peptidoglycan synthesis; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..668
FT                   /note="Penicillin-binding protein 3"
FT                   /id="PRO_0000195464"
FT   TRANSMEM        7..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        410
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AD65"
FT   CONFLICT        9
FT                   /note="V -> D (in Ref. 4; AAA22829)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        30..37
FT                   /note="RMEAFVKQ -> PLESTAQA (in Ref. 4; AAA22829)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   668 AA;  74406 MW;  A877A27B188ECAFC CRC64;
     MLKKCILLVF LCVGLIGLIG CSKTDSPEDR MEAFVKQWND QQFDDMYQSL TKDVKKEISK
     KDFVNRYKAI YEQAGVKNLK VTAGEVDKDD QDNKTMKHIP YKVSMNTNAG KVSFKNTAVL
     KLEKTDDEES WNIDWDPSFI FKQLADDKTV QIMSIEPKRG QIYDKNGKGL AVNTDVPEIG
     IVPGELGDKK EKVIKELAKK LDLTEDDIKK KLDQGWVKDD SFVPLKKVKP DQEKLVSEAT
     SLQGVTRTNV SSRYYPYGEK TAHLTGYVRA ITAEELKKKK EGTYSDTSNI GIAGLENVYE
     DKLRGTTGWK IYVPQTGEVI AEKKAKDGED LHLTIDIKTQ MKLYDELKDD SGAAVALQPK
     TGETLALVSA PSYDPNGFIF GWSDKEWKKL NKDKNNPFSA KFNKTYAPGS TIKPIAAAIG
     IKNGTLKADE KKTIKGKEWQ KDSSWGGYSV TRVSERLQQV DLENALITSD NIYFAQNALD
     MGADTFTKGL KTFGFSEDVP YEFPIQKSSI ANDKLDSDIL LADTGYGQGQ MQMSPLHLAT
     AYTPFVDNGD LVKPTLIKKD SQTADVWHKQ VVTKEGAADI TKGLKGVVED ERGSAYQPVV
     KGITVAGKTG TAELKTSKDD KDGTENGWFV GYDYENKDLL VAMMIQNVQD RGGSHYVVEK
     AKKQFQSN
//

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