(data stored in SCRATCH zone)

SWISSPROT: KIMA_BACSU

ID   KIMA_BACSU              Reviewed;         607 AA.
AC   P96589; Q797M3;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   11-DEC-2019, entry version 101.
DE   RecName: Full=Potassium transporter KimA {ECO:0000305};
DE   AltName: Full=K(+) importer A {ECO:0000303|PubMed:28420751};
GN   Name=kimA {ECO:0000303|PubMed:28420751}; Synonyms=ydaO;
GN   OrderedLocusNames=BSU04320;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDUCTION.
RX   PubMed=20511502; DOI=10.1128/jb.00450-10;
RA   Block K.F., Hammond M.C., Breaker R.R.;
RT   "Evidence for widespread gene control function by the ydaO riboswitch
RT   candidate.";
RL   J. Bacteriol. 192:3983-3989(2010).
RN   [4]
RP   INDUCTION.
RX   PubMed=24141192; DOI=10.1038/nchembio.1363;
RA   Nelson J.W., Sudarsan N., Furukawa K., Weinberg Z., Wang J.X.,
RA   Breaker R.R.;
RT   "Riboswitches in eubacteria sense the second messenger c-di-AMP.";
RL   Nat. Chem. Biol. 9:834-839(2013).
RN   [5]
RP   FUNCTION AS A TRANSPORTER, AND INDUCTION.
RX   PubMed=28420751; DOI=10.1126/scisignal.aal3011;
RA   Gundlach J., Herzberg C., Kaever V., Gunka K., Hoffmann T., Weiss M.,
RA   Gibhardt J., Thuermer A., Hertel D., Daniel R., Bremer E., Commichau F.M.,
RA   Stuelke J.;
RT   "Control of potassium homeostasis is an essential function of the second
RT   messenger cyclic di-AMP in Bacillus subtilis.";
RL   Sci. Signal. 10:0-0(2017).
CC   -!- FUNCTION: High-affinity potassium transporter.
CC       {ECO:0000269|PubMed:28420751}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Induced by low K(+) concentrations (PubMed:28420751).
CC       Expression is regulated by cyclic di-AMP (c-di-AMP), via the ydaO
CC       riboswitch (PubMed:20511502, PubMed:24141192, PubMed:28420751). High
CC       external concentrations of K(+) stimulate an increase in the production
CC       of c-di-AMP, which binds to the riboswitch and prevents kimA expression
CC       (PubMed:28420751). {ECO:0000269|PubMed:20511502,
CC       ECO:0000269|PubMed:24141192, ECO:0000269|PubMed:28420751}.
CC   -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC       superfamily. {ECO:0000305}.
DR   EMBL; AB001488; BAA19269.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12239.1; -; Genomic_DNA.
DR   PIR; F69769; F69769.
DR   RefSeq; NP_388313.1; NC_000964.3.
DR   RefSeq; WP_003246693.1; NZ_JNCM01000031.1.
DR   STRING; 224308.BSU04320; -.
DR   PaxDb; P96589; -.
DR   PRIDE; P96589; -.
DR   EnsemblBacteria; CAB12239; CAB12239; BSU04320.
DR   GeneID; 938237; -.
DR   KEGG; bsu:BSU04320; -.
DR   PATRIC; fig|224308.179.peg.458; -.
DR   eggNOG; ENOG4105C0X; Bacteria.
DR   eggNOG; COG0531; LUCA.
DR   HOGENOM; HOG000055696; -.
DR   InParanoid; P96589; -.
DR   OMA; HWYEHLL; -.
DR   PhylomeDB; P96589; -.
DR   BioCyc; BSUB:BSU04320-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR002293; AA/rel_permease1.
DR   Pfam; PF13520; AA_permease_2; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P96589.
DR   SWISS-2DPAGE; P96589.
KW   Cell membrane; Ion transport; Membrane; Potassium; Potassium transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..607
FT                   /note="Potassium transporter KimA"
FT                   /id="PRO_0000376842"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        408..428
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        430..450
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   607 AA;  66771 MW;  A307AEB08E72B372 CRC64;
     MYHSIKRFLI GKPLKSQAAG EQKLTKLKAL AMLSSDALSS VAYGTEQILI ILATISAAAF
     WYSIPIAVGV LILLLALILS YRQIIYAYPQ GGGAYIVSKE NLGEKPGLIA GGSLLVDYIL
     TVAVSISAGT DAITSAFPAL HDYHVPIAIF LVLVIMILNL RGLSESASIL AYPVYLFVVA
     LLVLIAVGLF KLMTGQIDQP AHHTSLGTPV AGITLFLLLK AFSSGCSALT GVEAISNAIP
     AFKNPPARNA ARTLAMMGIL LAILFSGITV LAYGYGTAPK PDETVVSQIA SETFGRNVFY
     YVIQGVTSLI LVLAANTGFS AFPQLAFNLA RDQYMPRMFT VRGDRLGFSN GIIFLGFASI
     VLIILFGGQT EHLIPLYAVG VFIPFTLSQT GMCMKWIKQK PKGWIGKMLI NSCGALISFM
     VLSILFVTKF NVVWPVLIFM PIVVLLFFAI KNHYTAVGEQ LRIVDKEPEE IKGTVVIVPV
     AGVTTVVQKS IHYAKSLSDQ VIAVHVSFDR EQEKKFEKRW EELNNGVRLV TLHSSYRSLV
     HPFDKFLETV EAKAKKEQFS VMVLFPQFIT KKRWHTILHN QSAFLLRVRL FWKKDIMVAT
     LPYHFKK
//

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