(data stored in ACNUC7421 zone)

SWISSPROT: YDAP_BACSU

ID   YDAP_BACSU              Reviewed;         574 AA.
AC   P96591; Q797M1;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   11-DEC-2019, entry version 109.
DE   RecName: Full=Putative thiamine pyrophosphate-containing protein YdaP;
GN   Name=ydaP; OrderedLocusNames=BSU04340;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   REGULATION.
RC   STRAIN=168;
RX   PubMed=10220166; DOI=10.1099/13500872-145-4-869;
RA   Petersohn A., Antelmann H., Gerth U., Hecker M.;
RT   "Identification and transcriptional analysis of new members of the sigmaB
RT   regulon in Bacillus subtilis.";
RL   Microbiology 145:869-880(1999).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000305};
CC   -!- COFACTOR:
CC       Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC         Evidence={ECO:0000305};
CC       Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000305};
CC   -!- INDUCTION: Transcriptionally regulated by SigB.
CC   -!- SIMILARITY: Belongs to the TPP enzyme family. {ECO:0000305}.
DR   EMBL; AB001488; BAA19271.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12241.1; -; Genomic_DNA.
DR   PIR; G69769; G69769.
DR   RefSeq; NP_388315.1; NC_000964.3.
DR   RefSeq; WP_003246569.1; NZ_JNCM01000031.1.
DR   SMR; P96591; -.
DR   IntAct; P96591; 1.
DR   STRING; 224308.BSU04340; -.
DR   PaxDb; P96591; -.
DR   PRIDE; P96591; -.
DR   EnsemblBacteria; CAB12241; CAB12241; BSU04340.
DR   GeneID; 938239; -.
DR   KEGG; bsu:BSU04340; -.
DR   PATRIC; fig|224308.179.peg.460; -.
DR   eggNOG; COG0028; LUCA.
DR   HOGENOM; HOG000258444; -.
DR   InParanoid; P96591; -.
DR   KO; K00158; -.
DR   OMA; RGKEWIQ; -.
DR   PhylomeDB; P96591; -.
DR   BioCyc; BSUB:BSU04340-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR   InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR   InterPro; IPR000399; TPP-bd_CS.
DR   InterPro; IPR011766; TPP_enzyme-bd_C.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
DR   Pfam; PF00205; TPP_enzyme_M; 1.
DR   Pfam; PF02776; TPP_enzyme_N; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   SUPFAM; SSF52518; SSF52518; 2.
DR   PROSITE; PS00187; TPP_ENZYMES; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; P96591.
DR   SWISS-2DPAGE; P96591.
KW   Coiled coil; FAD; Flavoprotein; Magnesium; Metal-binding;
KW   Reference proteome; Thiamine pyrophosphate.
FT   CHAIN           1..574
FT                   /note="Putative thiamine pyrophosphate-containing protein
FT                   YdaP"
FT                   /id="PRO_0000380114"
FT   NP_BIND         256..277
FT                   /note="FAD"
FT                   /evidence="ECO:0000250"
FT   NP_BIND         294..313
FT                   /note="FAD"
FT                   /evidence="ECO:0000250"
FT   REGION          384..464
FT                   /note="Thiamine pyrophosphate binding"
FT                   /evidence="ECO:0000250"
FT   COILED          28..55
FT                   /evidence="ECO:0000255"
FT   METAL           435
FT                   /note="Magnesium"
FT                   /evidence="ECO:0000250"
FT   METAL           462
FT                   /note="Magnesium"
FT                   /evidence="ECO:0000250"
FT   BINDING         52
FT                   /note="Thiamine pyrophosphate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   574 AA;  63138 MW;  F887A1271B7E9710 CRC64;
     MAHKTAGQAM TELLEQWGVD HVYGIPGDSI NEFIEELRHE RNQLKFIQTR HEEVAALAAA
     AEAKLTGKIG VCLSIAGPGA VHLLNGLYDA KADGAPVLAI AGQVSSGEVG RDYFQEIKLE
     QMFEDVAVFN REVHSAESLP DLLNQAIRTA YSKKGVAVLS VSDDLFAEKI KREPVYTSPV
     YIEGNLEPKK EQLVTCAQYI NNAKKPIILA GQGMKKAKRE LLEFADKAAA PIVVTLPAKG
     VVPDKHPHFL GNLGQIGTKP AYEAMEECDL LIMLGTSFPY RDYLPDDTPA IQLDSDPAKI
     GKRYPVTAGL VCDSALGLRE LTEYIERKED RRFLNACTEH MQHWWNEIEK DETEATTPLK
     PQQVVARLQE AAADDAVLSV DVGTVTVWMA RHFKMNANQD FIVSSWLATM GCGLPGAIAA
     SLSEPERQAI AVCGDGGFSM VMQDLPTAVK YKLPITVVIL NNENLGMIEY EQQVKGNIDY
     VTKLQNVDYA AFAESCGAKG IKVTKAEELA PAFHEALHSD QPVVVDVMIG NEPPLPGKIT
     YGQAKGFSKY MLKNFFENQK FEMPSLKKSL KRLF
//

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