(data stored in SCRATCH zone)

SWISSPROT: MCAT_BACSU

ID   MCAT_BACSU              Reviewed;         273 AA.
AC   P80878; P96599;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   11-DEC-2019, entry version 117.
DE   RecName: Full=Probable manganese catalase;
DE            EC=1.11.1.6;
DE   AltName: Full=General stress protein 80;
DE            Short=GSP80;
GN   Name=ydbD; OrderedLocusNames=BSU04430;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-29.
RC   STRAIN=168 / IS58;
RX   PubMed=9298659; DOI=10.1002/elps.1150180820;
RA   Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.;
RT   "First steps from a two-dimensional protein index towards a response-
RT   regulation map for Bacillus subtilis.";
RL   Electrophoresis 18:1451-1463(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.6;
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000250};
CC   -!- INDUCTION: By heat shock, salt stress, oxidative stress, glucose
CC       limitation and oxygen limitation.
CC   -!- SIMILARITY: Belongs to the manganese catalase family. {ECO:0000305}.
DR   EMBL; AB001488; BAA19280.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12250.1; -; Genomic_DNA.
DR   PIR; G69770; G69770.
DR   RefSeq; NP_388324.1; NC_000964.3.
DR   RefSeq; WP_003234352.1; NZ_JNCM01000031.1.
DR   SMR; P80878; -.
DR   STRING; 224308.BSU04430; -.
DR   PeroxiBase; 6145; BsMnCat.
DR   PaxDb; P80878; -.
DR   PRIDE; P80878; -.
DR   EnsemblBacteria; CAB12250; CAB12250; BSU04430.
DR   GeneID; 938232; -.
DR   KEGG; bsu:BSU04430; -.
DR   PATRIC; fig|224308.179.peg.469; -.
DR   eggNOG; COG3546; LUCA.
DR   HOGENOM; HOG000267706; -.
DR   InParanoid; P80878; -.
DR   OMA; NPHHAIV; -.
DR   PhylomeDB; P80878; -.
DR   BioCyc; BSUB:BSU04430-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0004096; F:catalase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01051; Mn_catalase; 1.
DR   Gene3D; 1.20.1260.10; -; 1.
DR   Gene3D; 3.30.1530.10; -; 1.
DR   InterPro; IPR012347; Ferritin-like.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR007760; Mn_catalase.
DR   InterPro; IPR027407; Mn_catalase_C.
DR   InterPro; IPR039377; Mn_catalase_dom.
DR   Pfam; PF05067; Mn_catalase; 1.
DR   SUPFAM; SSF47240; SSF47240; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P80878.
DR   SWISS-2DPAGE; P80878.
KW   Calcium; Direct protein sequencing; Manganese; Metal-binding;
KW   Oxidoreductase; Peroxidase; Reference proteome; Stress response.
FT   CHAIN           1..273
FT                   /note="Probable manganese catalase"
FT                   /id="PRO_0000096153"
FT   METAL           35
FT                   /note="Manganese 1"
FT                   /evidence="ECO:0000250"
FT   METAL           57
FT                   /note="Calcium"
FT                   /evidence="ECO:0000250"
FT   METAL           61
FT                   /note="Calcium"
FT                   /evidence="ECO:0000250"
FT   METAL           66
FT                   /note="Manganese 1"
FT                   /evidence="ECO:0000250"
FT   METAL           66
FT                   /note="Manganese 2"
FT                   /evidence="ECO:0000250"
FT   METAL           69
FT                   /note="Manganese 1"
FT                   /evidence="ECO:0000250"
FT   METAL           149
FT                   /note="Manganese 2"
FT                   /evidence="ECO:0000250"
FT   METAL           182
FT                   /note="Manganese 2"
FT                   /evidence="ECO:0000250"
FT   METAL           220
FT                   /note="Calcium"
FT                   /evidence="ECO:0000250"
FT   METAL           222
FT                   /note="Calcium; via carbonyl oxygen"
FT                   /evidence="ECO:0000250"
FT   METAL           224
FT                   /note="Calcium; via carbonyl oxygen"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   273 AA;  30257 MW;  C921368C8F1AC075 CRC64;
     MFKHTKMLQH PAKPDRPDPL FAKKMQEILG GQFGEISVAM QYLFQGWNTR GNEKYKDLLM
     DTATEELGHV EMIATMIARL LEDAPLDQQE KAAEDPVIGS ILGGMNPHHA IVSGLGAMPE
     SSTGVPWSGG YIVASGNLLA DFRANLNAES QGRLQVARLF EMTDDKGVKD MLSFLLARDT
     MHQNQWLAAI KELEAQEGPV VPGTFPKALE KQEFSHQLIN FSEGEVSAEQ NWLNEKAPDG
     EAFEYVKEAK TFGEKPELKP APPFVHNTLP GRE
//

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