(data stored in SCRATCH zone)

SWISSPROT: CML21_ARATH

ID   CML21_ARATH             Reviewed;         231 AA.
AC   Q52K82; O65587; Q0WSM7;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   11-DEC-2019, entry version 94.
DE   RecName: Full=Probable calcium-binding protein CML21;
DE   AltName: Full=Calmodulin-like protein 21;
GN   Name=CML21; OrderedLocusNames=At4g26470; ORFNames=M3E9.100;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT   "Arabidopsis cDNA clones.";
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   DOI=10.1046/j.1469-8137.2003.00845.x;
RA   McCormack E., Braam J.;
RT   "Calmodulins and related potential calcium sensors of Arabidopsis.";
RL   New Phytol. 159:585-598(2003).
CC   -!- FUNCTION: Potential calcium sensor. {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q52K82-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q52K82-2; Sequence=VSP_034552, VSP_034553;
CC   -!- CAUTION: Although assigned as a calmodulin family member by Ref.5, it
CC       only contains EF-hand domains. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA18223.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAB79502.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AL022223; CAA18223.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161565; CAB79502.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002687; AEE85204.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE85205.1; -; Genomic_DNA.
DR   EMBL; BT021986; AAY17423.1; -; mRNA.
DR   EMBL; AK227901; BAE99871.1; -; mRNA.
DR   PIR; T05057; T05057.
DR   RefSeq; NP_001078450.1; NM_001084981.2. [Q52K82-2]
DR   RefSeq; NP_194377.2; NM_118780.4. [Q52K82-1]
DR   SMR; Q52K82; -.
DR   STRING; 3702.AT4G26470.1; -.
DR   PaxDb; Q52K82; -.
DR   EnsemblPlants; AT4G26470.1; AT4G26470.1; AT4G26470. [Q52K82-1]
DR   EnsemblPlants; AT4G26470.2; AT4G26470.2; AT4G26470. [Q52K82-2]
DR   GeneID; 828753; -.
DR   Gramene; AT4G26470.1; AT4G26470.1; AT4G26470. [Q52K82-1]
DR   Gramene; AT4G26470.2; AT4G26470.2; AT4G26470. [Q52K82-2]
DR   KEGG; ath:AT4G26470; -.
DR   Araport; AT4G26470; -.
DR   TAIR; locus:2131458; AT4G26470.
DR   eggNOG; KOG0027; Eukaryota.
DR   eggNOG; COG5126; LUCA.
DR   HOGENOM; HOG000241952; -.
DR   InParanoid; Q52K82; -.
DR   KO; K13448; -.
DR   OrthoDB; 1096220at2759; -.
DR   PhylomeDB; Q52K82; -.
DR   PRO; PR:Q52K82; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q52K82; baseline and differential.
DR   Genevisible; Q52K82; AT.
DR   GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR   GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR   GO; GO:0019722; P:calcium-mediated signaling; IBA:GO_Central.
DR   CDD; cd00051; EFh; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13499; EF-hand_7; 2.
DR   SMART; SM00054; EFh; 4.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 4.
PE   2: Evidence at transcript level;
DR   PRODOM; Q52K82.
DR   SWISS-2DPAGE; Q52K82.
KW   Alternative splicing; Calcium; Metal-binding; Reference proteome; Repeat.
FT   CHAIN           1..231
FT                   /note="Probable calcium-binding protein CML21"
FT                   /id="PRO_0000342950"
FT   DOMAIN          54..89
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          90..125
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          145..180
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          181..216
FT                   /note="EF-hand 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   CA_BIND         67..78
FT                   /note="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   CA_BIND         158..169
FT                   /note="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   CA_BIND         194..205
FT                   /note="3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   COMPBIAS        218..225
FT                   /note="Poly-Glu"
FT   VAR_SEQ         191..211
FT                   /note="EEMDWDKNGMVNFKEFLFAFT -> GLFLNSLPLLLKLSSSLNQEY (in
FT                   isoform 2)"
FT                   /evidence="ECO:0000303|Ref.4"
FT                   /id="VSP_034552"
FT   VAR_SEQ         212..231
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.4"
FT                   /id="VSP_034553"
SQ   SEQUENCE   231 AA;  26770 MW;  2687137FCA02677E CRC64;
     MGGAVTKSET LQKEWVPETK LEAKIIEAVQ RRASRGTTMK SFNSIVLKFP KIDDGLRNCK
     AIFQEFDEDS NGSIDHTELK NCIRKLEISF DEEEINDLFK ACDINEDMGI TFTEFIVLLC
     LVYLLKDDSS TLQKKWTMGM PKLEPTFETL VDTFVFLDEN KDGYVSREEM VRAIDESGER
     SSGRIAMKRF EEMDWDKNGM VNFKEFLFAF TQWVGIDENE EEEEEDNNEK A
//

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