(data stored in SCRATCH zone)

SWISSPROT: WRK20_ARATH

ID   WRK20_ARATH             Reviewed;         557 AA.
AC   Q93WV0; Q8H1E9; Q94AP6; Q9SUA0;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   11-DEC-2019, entry version 131.
DE   RecName: Full=Probable WRKY transcription factor 20;
DE   AltName: Full=WRKY DNA-binding protein 20;
GN   Name=WRKY20; OrderedLocusNames=At4g26640; ORFNames=T15N24.90;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia; TISSUE=Flower;
RA   Ulker B., Kushnir S., Somssich I.E.;
RT   "Arabidopsis thaliana transcription factor WRKY20.";
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC       (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC       acting element. {ECO:0000250|UniProtKB:Q9SI37}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9SI37}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q93WV0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q93WV0-2; Sequence=VSP_007247, VSP_007248;
CC   -!- SIMILARITY: Belongs to the WRKY group I family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB43860.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAB79519.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AF425837; AAL13050.1; -; mRNA.
DR   EMBL; AL078465; CAB43860.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161565; CAB79519.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002687; AEE85230.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE85231.1; -; Genomic_DNA.
DR   EMBL; AY045892; AAK76566.1; -; mRNA.
DR   EMBL; AY150436; AAN12978.1; -; mRNA.
DR   PIR; T08930; T08930.
DR   RefSeq; NP_567752.1; NM_118798.2. [Q93WV0-2]
DR   RefSeq; NP_849450.1; NM_179119.5. [Q93WV0-1]
DR   SMR; Q93WV0; -.
DR   BioGrid; 14058; 14.
DR   IntAct; Q93WV0; 13.
DR   STRING; 3702.AT4G26640.2; -.
DR   iPTMnet; Q93WV0; -.
DR   PaxDb; Q93WV0; -.
DR   PRIDE; Q93WV0; -.
DR   EnsemblPlants; AT4G26640.1; AT4G26640.1; AT4G26640. [Q93WV0-2]
DR   EnsemblPlants; AT4G26640.2; AT4G26640.2; AT4G26640. [Q93WV0-1]
DR   GeneID; 828771; -.
DR   Gramene; AT4G26640.1; AT4G26640.1; AT4G26640. [Q93WV0-2]
DR   Gramene; AT4G26640.2; AT4G26640.2; AT4G26640. [Q93WV0-1]
DR   KEGG; ath:AT4G26640; -.
DR   Araport; AT4G26640; -.
DR   TAIR; locus:2133852; AT4G26640.
DR   eggNOG; ENOG410IFHZ; Eukaryota.
DR   eggNOG; ENOG4112BTF; LUCA.
DR   HOGENOM; HOG000242409; -.
DR   InParanoid; Q93WV0; -.
DR   OMA; HEMAGSA; -.
DR   OrthoDB; 337930at2759; -.
DR   PhylomeDB; Q93WV0; -.
DR   PRO; PR:Q93WV0; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q93WV0; baseline and differential.
DR   Genevisible; Q93WV0; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0044212; F:transcription regulatory region DNA binding; IPI:TAIR.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR   GO; GO:0009961; P:response to 1-aminocyclopropane-1-carboxylic acid; IEP:TAIR.
DR   GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR   Gene3D; 2.20.25.80; -; 2.
DR   InterPro; IPR003657; WRKY_dom.
DR   InterPro; IPR036576; WRKY_dom_sf.
DR   Pfam; PF03106; WRKY; 2.
DR   SMART; SM00774; WRKY; 2.
DR   SUPFAM; SSF118290; SSF118290; 2.
DR   PROSITE; PS50811; WRKY; 2.
PE   2: Evidence at transcript level;
DR   PRODOM; Q93WV0.
DR   SWISS-2DPAGE; Q93WV0.
KW   Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..557
FT                   /note="Probable WRKY transcription factor 20"
FT                   /id="PRO_0000133662"
FT   DNA_BIND        205..269
FT                   /note="WRKY 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT   DNA_BIND        375..440
FT                   /note="WRKY 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT   METAL           236
FT                   /note="Zinc"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   METAL           241
FT                   /note="Zinc"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   METAL           264
FT                   /note="Zinc; via pros nitrogen"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   METAL           266
FT                   /note="Zinc; via tele nitrogen"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   METAL           406
FT                   /note="Zinc"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   METAL           411
FT                   /note="Zinc"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   METAL           435
FT                   /note="Zinc; via pros nitrogen"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   METAL           437
FT                   /note="Zinc; via tele nitrogen"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT   VAR_SEQ         1..72
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14593172"
FT                   /id="VSP_007247"
FT   VAR_SEQ         73..76
FT                   /note="SNIK -> MILL (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14593172"
FT                   /id="VSP_007248"
FT   CONFLICT        108
FT                   /note="Q -> R (in Ref. 4; AAK76566)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   557 AA;  61034 MW;  6179384AA4CEF0D9 CRC64;
     MNPQANDRKE FQGDCSATGD LTAKHDSAGG NGGGGARYKL MSPAKLPISR STDITIPPGL
     SPTSFLESPV FISNIKPEPS PTTGSLFKPR PVHISASSSS YTGRGFHQNT FTEQKSSEFE
     FRPPASNMVY AELGKIRSEP PVHFQGQGHG SSHSPSSISD AAGSSSELSR PTPPCQMTPT
     SSDIPAGSDQ EESIQTSQND SRGSTPSILA DDGYNWRKYG QKHVKGSEFP RSYYKCTHPN
     CEVKKLFERS HDGQITDIIY KGTHDHPKPQ PGRRNSGGMA AQEERLDKYP SSTGRDEKGS
     GVYNLSNPNE QTGNPEVPPI SASDDGGEAA ASNRNKDEPD DDDPFSKRRR MEGAMEITPL
     VKPIREPRVV VQTLSEVDIL DDGYRWRKYG QKVVRGNPNP RSYYKCTAHG CPVRKHVERA
     SHDPKAVITT YEGKHDHDVP TSKSSSNHEI QPRFRPDETD TISLNLGVGI SSDGPNHASN
     EHQHQNQQLV NQTHPNGVNF RFVHASPMSS YYASLNSGMN QYGQRETKNE TQNGDISSLN
     NSSYPYPPNM GRVQSGP
//

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