(data stored in ACNUC1104 zone)

SWISSPROT: LPXB_BORPA

ID   LPXB_BORPA              Reviewed;         393 AA.
AC   Q7WA47;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   08-MAY-2019, entry version 73.
DE   RecName: Full=Lipid-A-disaccharide synthase {ECO:0000255|HAMAP-Rule:MF_00392};
DE            EC=2.4.1.182 {ECO:0000255|HAMAP-Rule:MF_00392};
GN   Name=lpxB {ECO:0000255|HAMAP-Rule:MF_00392}; Synonyms=pgsB;
GN   OrderedLocusNames=BPP1540;
OS   Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=257311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12822 / ATCC BAA-587 / NCTC 13253;
RX   PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J.,
RA   Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K.,
RA   Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C.,
RA   Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K.,
RA   Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K.,
RA   Unwin L., Whitehead S., Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
CC   -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC       diacylglucosamine-1-phosphate to form lipid A disaccharide, a
CC       precursor of lipid A, a phosphorylated glycolipid that anchors the
CC       lipopolysaccharide to the outer membrane of the cell.
CC       {ECO:0000255|HAMAP-Rule:MF_00392}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-N,3-O-bis(3-hydroxytetradecanoyl)-alpha-D-glucosaminyl
CC         1-phosphate + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-
CC         alpha-D-glucosamine = H(+) + lipid A disaccharide (E. coli) +
CC         UDP; Xref=Rhea:RHEA:22668, ChEBI:CHEBI:15378, ChEBI:CHEBI:57957,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58466, ChEBI:CHEBI:78847;
CC         EC=2.4.1.182; Evidence={ECO:0000255|HAMAP-Rule:MF_00392};
CC   -!- PATHWAY: Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid
CC       IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and
CC       UDP-N-acetyl-alpha-D-glucosamine: step 5/6. {ECO:0000255|HAMAP-
CC       Rule:MF_00392}.
CC   -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00392}.
DR   EMBL; BX640427; CAE36842.1; -; Genomic_DNA.
DR   RefSeq; WP_003811772.1; NC_002928.3.
DR   SMR; Q7WA47; -.
DR   CAZy; GT19; Glycosyltransferase Family 19.
DR   EnsemblBacteria; CAE36842; CAE36842; BPP1540.
DR   KEGG; bpa:BPP1540; -.
DR   HOGENOM; HOG000018003; -.
DR   KO; K00748; -.
DR   OMA; PTVWAWR; -.
DR   BioCyc; BPAR257311:G1GSY-1580-MONOMER; -.
DR   UniPathway; UPA00359; UER00481.
DR   Proteomes; UP000001421; Chromosome.
DR   GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00392; LpxB; 1.
DR   InterPro; IPR003835; Glyco_trans_19.
DR   PANTHER; PTHR30372; PTHR30372; 1.
DR   Pfam; PF02684; LpxB; 1.
DR   TIGRFAMs; TIGR00215; lpxB; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q7WA47.
DR   SWISS-2DPAGE; Q7WA47.
KW   Complete proteome; Glycosyltransferase; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Transferase.
FT   CHAIN         1    393       Lipid-A-disaccharide synthase.
FT                                /FTId=PRO_0000190153.
SQ   SEQUENCE   393 AA;  42748 MW;  C1482799F9CBC38C CRC64;
     MSLRIGMVAG EPSGDLLAGR IIAGLQARAP GVHCAGIGGP QMAARGFEAW HPMHALTVFG
     YIDAFKRIPS LLSTYGDVKR RLLAEPPSVF VGIDAPDFNL RLEHQLRQAG TPTVHFVGPS
     IWAWRYERIN KIRAAVSHML VLFPFEEALY RKEGIPVTYV GHPLAGVIPM QPDRAAARAR
     LGIDADARVL AILPGSRSSE IRLLAPRFLQ AAAELVRRDP RLQCVVPMVN PQRRAEFEAI
     AAQHPVPGLR CVTAAEGQGE TPVAWSVMEA SNAVLVASGT ATLETALYKR PMVISYVLSP
     WMRRIMAWKS GQQRPYLPWV GLPNVLLRDF AVPELLQDEA TPAALAEATW QALTDEAGAA
     RIEARFTALH QDLLRDTPAL AAQAILEVAD GAA
//

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