(data stored in ACNUC1104 zone)

SWISSPROT: SSRP_BORPA

ID   SSRP_BORPA              Reviewed;         155 AA.
AC   Q7WA41;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   08-MAY-2019, entry version 83.
DE   RecName: Full=SsrA-binding protein {ECO:0000255|HAMAP-Rule:MF_00023};
DE   AltName: Full=Small protein B {ECO:0000255|HAMAP-Rule:MF_00023};
GN   Name=smpB {ECO:0000255|HAMAP-Rule:MF_00023};
GN   OrderedLocusNames=BPP1549;
OS   Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=257311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12822 / ATCC BAA-587 / NCTC 13253;
RX   PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J.,
RA   Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K.,
RA   Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C.,
RA   Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K.,
RA   Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K.,
RA   Unwin L., Whitehead S., Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
CC   -!- FUNCTION: Required for rescue of stalled ribosomes mediated by
CC       trans-translation. Binds to transfer-messenger RNA (tmRNA),
CC       required for stable association of tmRNA with ribosomes. tmRNA and
CC       SmpB together mimic tRNA shape, replacing the anticodon stem-loop
CC       with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold
CC       to resemble tRNA(Ala) and it encodes a 'tag peptide', a short
CC       internal open reading frame. During trans-translation Ala-
CC       aminoacylated tmRNA acts like a tRNA, entering the A-site of
CC       stalled ribosomes, displacing the stalled mRNA. The ribosome then
CC       switches to translate the ORF on the tmRNA; the nascent peptide is
CC       terminated with the 'tag peptide' encoded by the tmRNA and
CC       targeted for degradation. The ribosome is freed to recommence
CC       translation, which seems to be the essential function of trans-
CC       translation. {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00023}.
CC       Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes.
CC       {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- SIMILARITY: Belongs to the SmpB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00023}.
DR   EMBL; BX640427; CAE36851.1; -; Genomic_DNA.
DR   RefSeq; WP_010928088.1; NC_002928.3.
DR   SMR; Q7WA41; -.
DR   EnsemblBacteria; CAE36851; CAE36851; BPP1549.
DR   KEGG; bpa:BPP1549; -.
DR   HOGENOM; HOG000009628; -.
DR   KO; K03664; -.
DR   OMA; KLHDKRA; -.
DR   BioCyc; BPAR257311:G1GSY-1589-MONOMER; -.
DR   Proteomes; UP000001421; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070929; P:trans-translation; IEA:UniProtKB-UniRule.
DR   CDD; cd09294; SmpB; 1.
DR   Gene3D; 2.40.280.10; -; 1.
DR   HAMAP; MF_00023; SmpB; 1.
DR   InterPro; IPR023620; SmpB.
DR   InterPro; IPR000037; SsrA-bd_prot.
DR   InterPro; IPR020081; SsrA-bd_prot_CS.
DR   PANTHER; PTHR30308; PTHR30308; 1.
DR   Pfam; PF01668; SmpB; 1.
DR   ProDom; PD004488; SmpB; 1.
DR   SUPFAM; SSF74982; SSF74982; 1.
DR   TIGRFAMs; TIGR00086; smpB; 1.
DR   PROSITE; PS01317; SSRP; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q7WA41.
DR   SWISS-2DPAGE; Q7WA41.
KW   Complete proteome; Cytoplasm; RNA-binding.
FT   CHAIN         1    155       SsrA-binding protein.
FT                                /FTId=PRO_0000102915.
SQ   SEQUENCE   155 AA;  18254 MW;  95B9EC7F608634C7 CRC64;
     MSIIDNRKAT HDYFIEDRYE AGMVLEGWEV KAIRDGRVHL KESYVIVRDG EIYLLGMHVS
     PLPTASTHIR PDATRTRKLL LKAEEIRKLI GKVEQRGYTL VPLNLHYKNG RIKLDFALGR
     GKKLYDKRDT AREKDWQREK ERVLKHDTRV NQRDS
//

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