(data stored in ACNUC1104 zone)

SWISSPROT: A4F6B1_SACEN

ID   A4F6B1_SACEN            Unreviewed;       155 AA.
AC   A4F6B1;
DT   17-APR-2007, integrated into UniProtKB/TrEMBL.
DT   17-APR-2007, sequence version 1.
DT   08-MAY-2019, entry version 67.
DE   RecName: Full=tRNA-specific adenosine deaminase {ECO:0000256|HAMAP-Rule:MF_00972};
DE            EC=3.5.4.33 {ECO:0000256|HAMAP-Rule:MF_00972};
GN   Name=tadA {ECO:0000256|HAMAP-Rule:MF_00972};
GN   OrderedLocusNames=SACE_0233 {ECO:0000313|EMBL:CAL99585.1};
GN   ORFNames=A8924_0625 {ECO:0000313|EMBL:PFG93387.1};
OS   Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748
OS   / NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharopolyspora.
OX   NCBI_TaxID=405948 {ECO:0000313|EMBL:CAL99585.1, ECO:0000313|Proteomes:UP000006728};
RN   [1] {ECO:0000313|EMBL:CAL99585.1, ECO:0000313|Proteomes:UP000006728}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 /
RC   NRRL 2338 {ECO:0000313|Proteomes:UP000006728}, and NRRL 2338
RC   {ECO:0000313|EMBL:CAL99585.1};
RX   PubMed=17369815; DOI=10.1038/nbt1297;
RA   Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N.,
RA   Dickens S., Haydock S.F., Leadlay P.F.;
RT   "Complete genome sequence of the erythromycin-producing bacterium
RT   Saccharopolyspora erythraea NRRL23338.";
RL   Nat. Biotechnol. 25:447-453(2007).
RN   [2] {ECO:0000313|EMBL:PFG93387.1, ECO:0000313|Proteomes:UP000225825}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 40517 {ECO:0000313|EMBL:PFG93387.1,
RC   ECO:0000313|Proteomes:UP000225825};
RA   Klenk H.-P.;
RT   "Sequencing the genomes of 1000 actinobacteria strains.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the deamination of adenosine to inosine at the
CC       wobble position 34 of tRNA(Arg2). {ECO:0000256|HAMAP-
CC       Rule:MF_00972}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA
CC         + NH4(+); Xref=Rhea:RHEA:43168, Rhea:RHEA-COMP:10373, Rhea:RHEA-
CC         COMP:10374, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:74411, ChEBI:CHEBI:82852;
CC         EC=3.5.4.33; Evidence={ECO:0000256|HAMAP-Rule:MF_00972};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00972};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00972};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00972}.
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DR   EMBL; AM420293; CAL99585.1; -; Genomic_DNA.
DR   EMBL; PDBV01000001; PFG93387.1; -; Genomic_DNA.
DR   RefSeq; WP_009944875.1; NZ_PDBV01000001.1.
DR   STRING; 405948.SACE_0233; -.
DR   EnsemblBacteria; CAL99585; CAL99585; SACE_0233.
DR   KEGG; sen:SACE_0233; -.
DR   eggNOG; ENOG4108Z6B; Bacteria.
DR   eggNOG; COG0590; LUCA.
DR   HOGENOM; HOG000085050; -.
DR   KO; K11991; -.
DR   OMA; LEPCQMC; -.
DR   OrthoDB; 1890883at2; -.
DR   BioCyc; SERY405948:SACE_RS01145-MONOMER; -.
DR   Proteomes; UP000006728; Chromosome.
DR   Proteomes; UP000225825; Unassembled WGS sequence.
DR   GO; GO:0052717; F:tRNA-specific adenosine-34 deaminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00972; tRNA_aden_deaminase; 1.
DR   InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR   InterPro; IPR002125; CMP_dCMP_dom.
DR   InterPro; IPR016193; Cytidine_deaminase-like.
DR   InterPro; IPR028883; tRNA_aden_deaminase.
DR   Pfam; PF14437; MafB19-deam; 1.
DR   SUPFAM; SSF53927; SSF53927; 1.
DR   PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR   PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; A4F6B1.
DR   SWISS-2DPAGE; A4F6B1.
KW   Complete proteome {ECO:0000313|Proteomes:UP000006728,
KW   ECO:0000313|Proteomes:UP000225825};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00972,
KW   ECO:0000313|EMBL:CAL99585.1};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006728};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   DOMAIN        6    119       CMP/dCMP-type deaminase.
FT                                {ECO:0000259|PROSITE:PS51747}.
FT   ACT_SITE     60     60       Proton donor. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        58     58       Zinc; via pros nitrogen; catalytic.
FT                                {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   METAL        89     89       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        92     92       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
SQ   SEQUENCE   155 AA;  16338 MW;  14ED4EE07A1FEB6D CRC64;
     MTAAGARDTE LVRAALEVAP GALSTGDVPI GAVVAGPDGR VLARDHNRRE ALHDPTAHAE
     ILALRAAAGV LGDGWRLEGC TLAVTVEPCT MCAGALVLAR VERVVFGCWE PRTGAVGSLW
     DVVRDRRLNH RPEVVGGVLE AECSALLEAF FHGQR
//

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