(data stored in SCRATCH zone)

SWISSPROT: C5DCR1_LACTC

ID   C5DCR1_LACTC            Unreviewed;       477 AA.
AC   C5DCR1;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-JUL-2009, sequence version 1.
DT   16-JAN-2019, entry version 57.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN   Name=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN   Synonyms=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN   OrderedLocusNames=KLTH0B05126g {ECO:0000313|EMBL:CAR21572.1};
OS   Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
OS   (Yeast) (Kluyveromyces thermotolerans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Lachancea.
OX   NCBI_TaxID=559295 {ECO:0000313|EMBL:CAR21572.1, ECO:0000313|Proteomes:UP000002036};
RN   [1] {ECO:0000313|EMBL:CAR21572.1, ECO:0000313|Proteomes:UP000002036}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 56472 / CBS 6340 / NRRL Y-8284
RC   {ECO:0000313|Proteomes:UP000002036};
RX   PubMed=19525356; DOI=10.1101/gr.091546.109;
RG   The Genolevures Consortium;
RA   Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V.,
RA   Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P.,
RA   Jubin C., Poulain J., Barbe V., Segurens B., Artiguenave F.,
RA   Anthouard V., Vacherie B., Val M.-E., Fulton R.S., Minx P., Wilson R.,
RA   Durrens P., Jean G., Marck C., Martin T., Nikolski M., Rolland T.,
RA   Seret M.-L., Casaregola S., Despons L., Fairhead C., Fischer G.,
RA   Lafontaine I., Leh V., Lemaire M., de Montigny J., Neuveglise C.,
RA   Thierry A., Blanc-Lenfle I., Bleykasten C., Diffels J., Fritsch E.,
RA   Frangeul L., Goeffon A., Jauniaux N., Kachouri-Lafond R., Payen C.,
RA   Potier S., Pribylova L., Ozanne C., Richard G.-F., Sacerdot C.,
RA   Straub M.-L., Talla E.;
RT   "Comparative genomics of protoploid Saccharomycetaceae.";
RL   Genome Res. 19:1696-1709(2009).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at
CC       tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May
CC       act by forming a heterodimer with NCS6 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble
CC       position. Prior mcm(5) tRNA modification by the elongator complex
CC       is required for 2-thiolation. May also be involved in protein
CC       urmylation. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-
CC       tRNA biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054,
CC       ECO:0000256|SAAS:SAAS00947298}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054,
CC       ECO:0000256|SAAS:SAAS00947297}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_03054, ECO:0000256|SAAS:SAAS00947301}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; CU928166; CAR21572.1; -; Genomic_DNA.
DR   RefSeq; XP_002552010.1; XM_002551964.1.
DR   STRING; 381046.XP_002552010.1; -.
DR   EnsemblFungi; CAR21572; CAR21572; KLTH0B05126g.
DR   GeneID; 8290848; -.
DR   KEGG; lth:KLTH0B05126g; -.
DR   HOGENOM; HOG000094395; -.
DR   InParanoid; C5DCR1; -.
DR   KO; K14169; -.
DR   OMA; CYGCKRS; -.
DR   OrthoDB; 1442062at2759; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000002036; Chromosome B.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5DCR1.
DR   SWISS-2DPAGE; C5DCR1.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002036};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054,
KW   ECO:0000256|SAAS:SAAS00947296};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002036};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_03054,
KW   ECO:0000256|SAAS:SAAS00947300}.
SQ   SEQUENCE   477 AA;  53904 MW;  C7F6ABC3653864C2 CRC64;
     MGINQLCKRC KTAEAAVVSR KEPFCIACFR KFVSLKQRKQ MMSDQYFQDI FKVMYPDKKK
     TAEEAERLNQ GSRILVPLSF GSSSIVMLDV LNDTLTEQRE MHRGKTGFRI DVLVCYLKDD
     LEELKRKAEA LMTVKLRDNK NSVKVHLVDI ESSMNSPDFT RIHLQDSTYM VRNASVSANE
     AQSVKELLSS CSNRSAREDL LNIIKTAIIK RFGVQGGYKA ILWGHSTTRL ADEIMSLVVK
     GRASQIANAL NDSEEDFEYG EPLRNLHPMR DILLSEIDAY CFVSDLTQYL HKYKPQDTLL
     VNKIPVAPDA QPPRAKLTKN MTINELARQY FDGIEGSYSN VISTVVRTGA KLDDPENSLK
     SKVRCGICNN TVFCDGSHWL RNITVTSSHP IEDAEEEAMF RAWSASEQGK VRGEYLKLTS
     EMEISGHEFP VCYGCILTLG EVKHKSLIWP SSQSNSVESV IEDFIISDES DSEHEGH
//

If you have problems or comments...

PBIL Back to PBIL home page