(data stored in ACNUC7421 zone)

SWISSPROT: B7UII7_ECO27

ID   B7UII7_ECO27            Unreviewed;       454 AA.
AC   B7UII7;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 60.
DE   RecName: Full=Poly(A) polymerase I {ECO:0000256|HAMAP-Rule:MF_00957};
DE            Short=PAP I {ECO:0000256|HAMAP-Rule:MF_00957};
DE            EC=2.7.7.19 {ECO:0000256|HAMAP-Rule:MF_00957};
GN   Name=pcnB {ECO:0000256|HAMAP-Rule:MF_00957,
GN   ECO:0000313|EMBL:CAS07694.1};
GN   OrderedLocusNames=E2348C_0146 {ECO:0000313|EMBL:CAS07694.1};
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521 {ECO:0000313|EMBL:CAS07694.1, ECO:0000313|Proteomes:UP000008205};
RN   [1] {ECO:0000313|EMBL:CAS07694.1, ECO:0000313|Proteomes:UP000008205}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC {ECO:0000313|Proteomes:UP000008205};
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Adds poly(A) tail to the 3' end of many RNAs, which
CC       usually targets these RNAs for decay. Plays a significant role in
CC       the global control of gene expression, through influencing the
CC       rate of transcript degradation, and in the general RNA quality
CC       control. {ECO:0000256|HAMAP-Rule:MF_00957}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine
CC         ribonucleotide; Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:11128,
CC         Rhea:RHEA-COMP:14647, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:83400, ChEBI:CHEBI:140626; EC=2.7.7.19;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00957};
CC   -!- SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A)
CC       polymerase family. {ECO:0000256|HAMAP-Rule:MF_00957,
CC       ECO:0000256|RuleBase:RU003953, ECO:0000256|SAAS:SAAS00577624}.
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DR   EMBL; FM180568; CAS07694.1; -; Genomic_DNA.
DR   EnsemblBacteria; CAS07694; CAS07694; E2348C_0146.
DR   KEGG; ecg:E2348C_0146; -.
DR   HOGENOM; HOG000256527; -.
DR   KO; K00970; -.
DR   OMA; FMAKLDM; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006378; P:mRNA polyadenylation; IEA:InterPro.
DR   CDD; cd05398; NT_ClassII-CCAase; 1.
DR   HAMAP; MF_00957; PolyA_pol; 1.
DR   InterPro; IPR002646; PolA_pol_head_dom.
DR   InterPro; IPR010206; PolA_pol_I.
DR   InterPro; IPR025866; PolyA_pol_arg_C_dom.
DR   InterPro; IPR032828; PolyA_RNA-bd.
DR   Pfam; PF01743; PolyA_pol; 1.
DR   Pfam; PF12626; PolyA_pol_arg_C; 1.
DR   Pfam; PF12627; PolyA_pol_RNAbd; 1.
DR   TIGRFAMs; TIGR01942; pcnB; 1.
PE   3: Inferred from homology;
DR   PRODOM; B7UII7.
DR   SWISS-2DPAGE; B7UII7.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00957,
KW   ECO:0000256|SAAS:SAAS00415369};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008205};
KW   mRNA processing {ECO:0000256|HAMAP-Rule:MF_00957};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00957,
KW   ECO:0000256|SAAS:SAAS00415411};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_00957,
KW   ECO:0000256|RuleBase:RU003953, ECO:0000256|SAAS:SAAS00468387};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00957};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00957,
KW   ECO:0000256|RuleBase:RU003953, ECO:0000256|SAAS:SAAS00468438}.
FT   DOMAIN       51    182       PolyA_pol. {ECO:0000259|Pfam:PF01743}.
FT   DOMAIN      209    269       PolyA_pol_RNAbd. {ECO:0000259|Pfam:
FT                                PF12627}.
FT   DOMAIN      327    445       PolyA_pol_arg_C. {ECO:0000259|Pfam:
FT                                PF12626}.
FT   ACT_SITE     69     69       {ECO:0000256|HAMAP-Rule:MF_00957}.
FT   ACT_SITE     71     71       {ECO:0000256|HAMAP-Rule:MF_00957}.
FT   ACT_SITE    151    151       {ECO:0000256|HAMAP-Rule:MF_00957}.
SQ   SEQUENCE   454 AA;  52548 MW;  C8815DFCD549196C CRC64;
     MLSREESEAE QAVARPQVTV IPREQHAISR KDISENALKV MYRLNKAGYE AWLVGGGVRD
     LLLGKKPKDF DVTTNATPEQ VRKLFRNCRL VGRRFRLAHV MFGPEIIEVA TFRGHHEGNV
     SDRTTSQRGQ NGMLLRDNIF GSIEEDAQRR DFTINSLYYS VADFTVRDYV GGMKDLKDGV
     IRLIGNPETR YREDPVRMLR AVRFAAKLGM RISPETAEPI PRLATLLNDI PPARLFEESL
     KLLQAGYGYE TYKLLCEYHL FQPLFPTITR YFTENGDSPM ERIIEQVLKN TDTRIHNDMR
     VNPAFLFAAM FWYPLLETAQ KIAQESGLTY HDAFALAMND VLDEACRSLA IPKRLTTLTR
     DIWQLQLRMS RRQGKRAWKL LEHPKFRAAY DLLALRAEVE RNAELQRLVK WWGEFQVSAP
     PDQKGMLNEL DEEPSPRRRT RRPRKRAPRR EGTA
//

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