(data stored in ACNUC7421 zone)

SWISSPROT: B7UIK5_ECO27

ID   B7UIK5_ECO27            Unreviewed;       266 AA.
AC   B7UIK5;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 55.
DE   RecName: Full=Vitamin B12-binding protein {ECO:0000256|HAMAP-Rule:MF_01000};
DE   Flags: Precursor;
GN   Name=btuF {ECO:0000256|HAMAP-Rule:MF_01000,
GN   ECO:0000313|EMBL:CAS07713.1};
GN   OrderedLocusNames=E2348C_0165 {ECO:0000313|EMBL:CAS07713.1};
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521 {ECO:0000313|EMBL:CAS07713.1, ECO:0000313|Proteomes:UP000008205};
RN   [1] {ECO:0000313|EMBL:CAS07713.1, ECO:0000313|Proteomes:UP000008205}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC {ECO:0000313|Proteomes:UP000008205};
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Part of the ABC transporter complex BtuCDF involved in
CC       vitamin B12 import. Binds vitamin B12 and delivers it to the
CC       periplasmic surface of BtuC. {ECO:0000256|HAMAP-Rule:MF_01000}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins
CC       (BtuD), two transmembrane proteins (BtuC) and a solute-binding
CC       protein (BtuF). {ECO:0000256|HAMAP-Rule:MF_01000}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000256|HAMAP-Rule:MF_01000}.
CC   -!- SIMILARITY: Belongs to the BtuF family. {ECO:0000256|HAMAP-
CC       Rule:MF_01000}.
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DR   EMBL; FM180568; CAS07713.1; -; Genomic_DNA.
DR   RefSeq; WP_001339555.1; NC_011601.1.
DR   EnsemblBacteria; CAS07713; CAS07713; E2348C_0165.
DR   KEGG; ecg:E2348C_0165; -.
DR   HOGENOM; HOG000282913; -.
DR   KO; K06858; -.
DR   OMA; WQGINLE; -.
DR   BioCyc; ECOL574521:E2348C_RS00850-MONOMER; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0015889; P:cobalamin transport; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01000; BtuF; 1.
DR   InterPro; IPR002491; ABC_transptr_periplasmic_BD.
DR   InterPro; IPR023544; ABC_transptr_vit_B12-bd.
DR   Pfam; PF01497; Peripla_BP_2; 1.
DR   PROSITE; PS50983; FE_B12_PBP; 1.
PE   3: Inferred from homology;
DR   PRODOM; B7UIK5.
DR   SWISS-2DPAGE; B7UIK5.
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008205};
KW   Disulfide bond {ECO:0000256|HAMAP-Rule:MF_01000};
KW   Periplasm {ECO:0000256|HAMAP-Rule:MF_01000};
KW   Signal {ECO:0000256|HAMAP-Rule:MF_01000};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01000}.
FT   SIGNAL        1     22       {ECO:0000256|HAMAP-Rule:MF_01000}.
FT   CHAIN        23    266       Vitamin B12-binding protein.
FT                                {ECO:0000256|HAMAP-Rule:MF_01000}.
FT                                /FTId=PRO_5009008045.
FT   DOMAIN       25    266       Fe/B12 periplasmic-binding.
FT                                {ECO:0000259|PROSITE:PS50983}.
FT   REGION      242    246       Cobalamin-binding. {ECO:0000256|HAMAP-
FT                                Rule:MF_01000}.
FT   COILED      136    156       {ECO:0000256|SAM:Coils}.
FT   BINDING      50     50       Cobalamin. {ECO:0000256|HAMAP-Rule:
FT                                MF_01000}.
FT   SITE         72     72       Important for BtuC binding.
FT                                {ECO:0000256|HAMAP-Rule:MF_01000}.
FT   SITE        202    202       Important for BtuC binding.
FT                                {ECO:0000256|HAMAP-Rule:MF_01000}.
FT   DISULFID    183    259       {ECO:0000256|HAMAP-Rule:MF_01000}.
SQ   SEQUENCE   266 AA;  29406 MW;  E94E044B6E6F96F7 CRC64;
     MAKSLFRALV ALSFLAPLWL YAAPRVITLS PANTELAFAA GITPVGVSSY SDYPPQAQKI
     EQVSTWQGMN LERIVALKPD LVIAWRGGNA ERQVDQLASL GIKVMWVDAT SIEQIANALR
     QLAPWSPQPD KAEKAAQSLL DQYAQLKAQY ADKSKKRVFL QFGINPPFTS GKESIQNQVL
     EVCGGENIFK DSRVPWPQVS REQVLARSPQ AIVITGGPDQ IPKIKQYWGE QLKIPVIPLT
     SDWFERASPR IILAAQQLCN ALSQVD
//

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