(data stored in ACNUC7421 zone)

SWISSPROT: RNH_ECO27

ID   RNH_ECO27               Reviewed;         155 AA.
AC   B7UJB0;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 53.
DE   RecName: Full=Ribonuclease H {ECO:0000255|HAMAP-Rule:MF_00042};
DE            Short=RNase H {ECO:0000255|HAMAP-Rule:MF_00042};
DE            EC=3.1.26.4 {ECO:0000255|HAMAP-Rule:MF_00042};
GN   Name=rnhA {ECO:0000255|HAMAP-Rule:MF_00042};
GN   OrderedLocusNames=E2348C_0214;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Endonuclease that specifically degrades the RNA of RNA-
CC       DNA hybrids. {ECO:0000255|HAMAP-Rule:MF_00042}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.;
CC         EC=3.1.26.4; Evidence={ECO:0000255|HAMAP-Rule:MF_00042};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00042};
CC       Note=Binds 1 Mg(2+) ion per subunit. May bind a second metal ion
CC       at a regulatory site, or after substrate binding.
CC       {ECO:0000255|HAMAP-Rule:MF_00042};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00042}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00042}.
CC   -!- SIMILARITY: Belongs to the RNase H family. {ECO:0000255|HAMAP-
CC       Rule:MF_00042}.
DR   EMBL; FM180568; CAS07762.1; -; Genomic_DNA.
DR   RefSeq; WP_000917867.1; NC_011601.1.
DR   SMR; B7UJB0; -.
DR   PRIDE; B7UJB0; -.
DR   EnsemblBacteria; CAS07762; CAS07762; E2348C_0214.
DR   KEGG; ecg:E2348C_0214; -.
DR   KO; K03469; -.
DR   OMA; MQEIEIF; -.
DR   BioCyc; ECOL574521:E2348C_RS01130-MONOMER; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd09278; RNase_HI_prokaryote_like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00042; RNase_H; 1.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR002156; RNaseH_domain.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR022892; RNaseHI.
DR   Pfam; PF00075; RNase_H; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50879; RNASE_H; 1.
PE   3: Inferred from homology;
DR   PRODOM; B7UJB0.
DR   SWISS-2DPAGE; B7UJB0.
KW   Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Magnesium;
KW   Metal-binding; Nuclease.
FT   CHAIN         1    155       Ribonuclease H.
FT                                /FTId=PRO_1000194433.
FT   DOMAIN        1    142       RNase H. {ECO:0000255|HAMAP-
FT                                Rule:MF_00042}.
FT   METAL        10     10       Magnesium 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00042}.
FT   METAL        10     10       Magnesium 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00042}.
FT   METAL        48     48       Magnesium 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00042}.
FT   METAL        70     70       Magnesium 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00042}.
FT   METAL       134    134       Magnesium 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00042}.
SQ   SEQUENCE   155 AA;  17578 MW;  B8EF940EBD1956BE CRC64;
     MLKQVEIFTD GSCLGNPGPG GYGAILRYRG HEKTFSAGYT RTTNNRMELM AAIVALEALK
     EHCEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW
     VKGHAGHPEN ERCDELARAA AMNPTLEDTG YQVEV
//

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