(data stored in ACNUC7421 zone)

SWISSPROT: ECPR_ECO27

ID   ECPR_ECO27              Reviewed;         196 AA.
AC   B7UJE2;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 59.
DE   RecName: Full=HTH-type transcriptional regulator EcpR;
GN   Name=ecpR; OrderedLocusNames=E2348C_0250;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
RN   [2]
RP   FUNCTION, DNA-BINDING, INDUCTION, AND MUTAGENESIS OF ASP-60; GLY-159;
RP   THR-175; VAL-176 AND LYS-186.
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=22797761; DOI=10.1128/JB.00915-12;
RA   Martinez-Santos V.I., Medrano-Lopez A., Saldana Z., Giron J.A.,
RA   Puente J.L.;
RT   "Transcriptional regulation of the ecp operon by EcpR, IHF, and H-NS
RT   in attaching and effacing Escherichia coli.";
RL   J. Bacteriol. 194:5020-5033(2012).
CC   -!- FUNCTION: Part of the ecpRABCDE operon, which encodes the E.coli
CC       common pilus (ECP). ECP is found in both commensal and pathogenic
CC       strains and plays a dual role in early-stage biofilm development
CC       and host cell recognition (By similarity). Positively regulates
CC       the expression of the ecp operon by binding to two TTCCT boxes.
CC       {ECO:0000250, ECO:0000269|PubMed:22797761}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Negatively regulated by H-NS. Positively autoregulated.
CC       Also positively regulated by IHF. {ECO:0000269|PubMed:22797761}.
CC   -!- SIMILARITY: Belongs to the EcpR/MatA family. {ECO:0000305}.
DR   EMBL; FM180568; CAS07798.1; -; Genomic_DNA.
DR   RefSeq; WP_000220221.1; NC_011601.1.
DR   SMR; B7UJE2; -.
DR   EnsemblBacteria; CAS07798; CAS07798; E2348C_0250.
DR   KEGG; ecg:E2348C_0250; -.
DR   HOGENOM; HOG000059412; -.
DR   KO; K21963; -.
DR   OMA; CIWPAHD; -.
DR   BioCyc; ECOL574521:E2348C_RS01325-MONOMER; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   1: Evidence at protein level;
DR   PRODOM; B7UJE2.
DR   SWISS-2DPAGE; B7UJE2.
KW   Activator; Complete proteome; Cytoplasm; DNA-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    196       HTH-type transcriptional regulator EcpR.
FT                                /FTId=PRO_0000429480.
FT   DOMAIN      138    196       HTH luxR-type. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00411}.
FT   DNA_BIND    162    181       H-T-H motif. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00411}.
FT   MUTAGEN      60     60       D->A: Decrease in activity.
FT                                {ECO:0000269|PubMed:22797761}.
FT   MUTAGEN     159    159       G->A: Decrease in activity.
FT                                {ECO:0000269|PubMed:22797761}.
FT   MUTAGEN     175    175       T->A: Decrease in activity.
FT                                {ECO:0000269|PubMed:22797761}.
FT   MUTAGEN     176    176       V->A: Lack of activity.
FT                                {ECO:0000269|PubMed:22797761}.
FT   MUTAGEN     186    186       K->A: Lack of activity.
FT                                {ECO:0000269|PubMed:22797761}.
SQ   SEQUENCE   196 AA;  23299 MW;  424D68D2B471154B CRC64;
     MTWQNDYSRD YEVKNHMECQ NRSDKYIWSP HDAYFYKGLS ELIVDIDRLI YLSLEKIRKD
     FVFINLNTDS LTEFINRDNE WLSAVKGKQV VLIAARKSEA LANYWYYNSN IRGVVYAGLS
     RDIRKELAYV INGRFLRKDI KKDKITDREM EIIRMTAQGM LPKSIARIEN CSVKTVYTHR
     RNAEAKLYSK LYKLVQ
//

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