(data stored in ACNUC7421 zone)

SWISSPROT: QUEC_ECO27

ID   QUEC_ECO27              Reviewed;         231 AA.
AC   B7UJR7;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 65.
DE   RecName: Full=7-cyano-7-deazaguanine synthase {ECO:0000255|HAMAP-Rule:MF_01633};
DE            EC=6.3.4.20 {ECO:0000255|HAMAP-Rule:MF_01633};
DE   AltName: Full=7-cyano-7-carbaguanine synthase {ECO:0000255|HAMAP-Rule:MF_01633};
DE   AltName: Full=PreQ(0) synthase {ECO:0000255|HAMAP-Rule:MF_01633};
DE   AltName: Full=Queuosine biosynthesis protein QueC {ECO:0000255|HAMAP-Rule:MF_01633};
GN   Name=queC {ECO:0000255|HAMAP-Rule:MF_01633};
GN   OrderedLocusNames=E2348C_0379;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Catalyzes the ATP-dependent conversion of 7-carboxy-7-
CC       deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).
CC       {ECO:0000255|HAMAP-Rule:MF_01633}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-
CC         deazaguanine + ADP + H(+) + H2O + phosphate;
CC         Xref=Rhea:RHEA:27982, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:45075, ChEBI:CHEBI:61036, ChEBI:CHEBI:456216;
CC         EC=6.3.4.20; Evidence={ECO:0000255|HAMAP-Rule:MF_01633};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01633};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_01633};
CC   -!- PATHWAY: Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01633}.
CC   -!- SIMILARITY: Belongs to the QueC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01633}.
DR   EMBL; FM180568; CAS07927.1; -; Genomic_DNA.
DR   RefSeq; WP_000817225.1; NC_011601.1.
DR   SMR; B7UJR7; -.
DR   EnsemblBacteria; CAS07927; CAS07927; E2348C_0379.
DR   KEGG; ecg:E2348C_0379; -.
DR   KO; K06920; -.
DR   OMA; VWVPARN; -.
DR   BioCyc; ECOL574521:E2348C_RS01990-MONOMER; -.
DR   UniPathway; UPA00391; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01995; ExsB; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_01633; QueC; 1.
DR   InterPro; IPR018317; QueC.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR42914; PTHR42914; 1.
DR   Pfam; PF06508; QueC; 1.
DR   PIRSF; PIRSF006293; ExsB; 1.
DR   TIGRFAMs; TIGR00364; TIGR00364; 1.
PE   3: Inferred from homology;
DR   PRODOM; B7UJR7.
DR   SWISS-2DPAGE; B7UJR7.
KW   ATP-binding; Complete proteome; Ligase; Metal-binding;
KW   Nucleotide-binding; Queuosine biosynthesis; Zinc.
FT   CHAIN         1    231       7-cyano-7-deazaguanine synthase.
FT                                /FTId=PRO_1000186587.
FT   NP_BIND       8     18       ATP. {ECO:0000255|HAMAP-Rule:MF_01633}.
FT   METAL       188    188       Zinc. {ECO:0000255|HAMAP-Rule:MF_01633}.
FT   METAL       197    197       Zinc. {ECO:0000255|HAMAP-Rule:MF_01633}.
FT   METAL       200    200       Zinc. {ECO:0000255|HAMAP-Rule:MF_01633}.
FT   METAL       203    203       Zinc. {ECO:0000255|HAMAP-Rule:MF_01633}.
SQ   SEQUENCE   231 AA;  25434 MW;  C621CDD03E6E3E3B CRC64;
     MKRAVVVFSG GQDSTTCLVQ ALQQYDEVHC VTFDYGQRHR AEIDVARELA LKLGARAHKV
     LDVTLLNELA VSSLTRDSIP VPDYEPEADG IPNTFVPGRN ILFLTLAAIY AYQVKAEAVI
     TGVCETDFSG YPDCRDEFVK ALNHAVSLGM AKDIRFETPL MWIDKAETWA LADYYGKLDL
     VRNETLTCYN GIKGDGCGHC AACNLRANGL NHYLADKPTV MAAIKQKTGL K
//

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