(data stored in ACNUC7421 zone)

SWISSPROT: B7UKQ7_ECO27

ID   B7UKQ7_ECO27            Unreviewed;       510 AA.
AC   B7UKQ7;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 48.
DE   RecName: Full=Citrate lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE            Short=Citrase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE            EC=2.8.3.10 {ECO:0000256|PIRNR:PIRNR009451};
DE            EC=4.1.3.6 {ECO:0000256|PIRNR:PIRNR009451};
DE   AltName: Full=Citrate (pro-3S)-lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE   AltName: Full=Citrate CoA-transferase subunit {ECO:0000256|PIRNR:PIRNR009451};
GN   Name=citF {ECO:0000313|EMBL:CAS08064.1};
GN   OrderedLocusNames=E2348C_0516 {ECO:0000313|EMBL:CAS08064.1};
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521 {ECO:0000313|EMBL:CAS08064.1, ECO:0000313|Proteomes:UP000008205};
RN   [1] {ECO:0000313|EMBL:CAS08064.1, ECO:0000313|Proteomes:UP000008205}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC {ECO:0000313|Proteomes:UP000008205};
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + citrate = (3S)-citryl-CoA + acetate;
CC         Xref=Rhea:RHEA:19405, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57321; EC=2.8.3.10;
CC         Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=citrate = acetate + oxaloacetate; Xref=Rhea:RHEA:10760,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089;
CC         EC=4.1.3.6; Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR009451}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; FM180568; CAS08064.1; -; Genomic_DNA.
DR   RefSeq; WP_000192238.1; NC_011601.1.
DR   EnsemblBacteria; CAS08064; CAS08064; E2348C_0516.
DR   KEGG; ecg:E2348C_0516; -.
DR   HOGENOM; HOG000117923; -.
DR   KO; K01643; -.
DR   OMA; GHCDVAA; -.
DR   BioCyc; ECOL574521:E2348C_RS02710-MONOMER; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0009346; C:citrate lyase complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0008815; F:citrate (pro-3S)-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008814; F:citrate CoA-transferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:UniProtKB-UniRule.
DR   InterPro; IPR006472; Citrate_lyase_asu.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR40596; PTHR40596; 1.
DR   Pfam; PF04223; CitF; 1.
DR   PIRSF; PIRSF009451; Citrt_lyas_alpha; 1.
DR   SUPFAM; SSF100950; SSF100950; 2.
DR   TIGRFAMs; TIGR01584; citF; 1.
PE   4: Predicted;
DR   PRODOM; B7UKQ7.
DR   SWISS-2DPAGE; B7UKQ7.
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008205};
KW   Cytoplasm {ECO:0000256|PIRNR:PIRNR009451};
KW   Lyase {ECO:0000256|PIRNR:PIRNR009451, ECO:0000313|EMBL:CAS08064.1};
KW   Transferase {ECO:0000256|PIRNR:PIRNR009451,
KW   ECO:0000313|EMBL:CAS08064.1}.
FT   COILED       33     53       {ECO:0000256|SAM:Coils}.
SQ   SEQUENCE   510 AA;  55161 MW;  FAF38151DC8CAB77 CRC64;
     MTQKIEQSQR QERVAAWNRR AECDLAAFQN SPKQTYQAEK ARDRKLCANL EEAIRRSGLQ
     DGMTVSFHHA FRGGDLTVNM VMDVIAKMGF KNLTLASSSL SDCHAPLVEH IRQGVVTRIY
     TSGLRGPLAE EISRGLLAEP VQIHSHGGRV HLVQSGELNI DVAFLGVPSC DEFGNANGYS
     GKACCGSLGY AMVDADNAKQ VVMLTEELLP YPHNPASIEQ DQVDLIVKVD RVGDAAKIGA
     GATRMTTNPR ELLIARSAAD VIVNSGYFKE GFSMQTGTGG ASLAVTRFLE DKMRSRDIRA
     DFALGGITAT MVDLHEKGLI RKLLDVQSFD SHAAQSLARN PNHIEISANQ YANWGSKGAA
     VDRLDVVVLS ALEIDTQFNV NVLTGSDGVL RGASGGHCDT AIASALSIIV APLVRGRIPT
     LVDNVLTCIT PGSSVDILVT DHGIAVNPAR PELAERLQEA GIKVVSIEWL RERARLLTGE
     PQPIEFTDRV VAVVRYRDGS VIDVVHQVKE
//

If you have problems or comments...

PBIL Back to PBIL home page