(data stored in ACNUC1104 zone)

SWISSPROT: D3UYU2_XENBS

ID   D3UYU2_XENBS            Unreviewed;       298 AA.
AC   D3UYU2;
DT   20-APR-2010, integrated into UniProtKB/TrEMBL.
DT   20-APR-2010, sequence version 1.
DT   08-MAY-2019, entry version 59.
DE   RecName: Full=tRNA dimethylallyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            EC=2.5.1.75 {ECO:0000256|HAMAP-Rule:MF_00185};
DE   AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=DMAPP:tRNA dimethylallyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=DMATase {ECO:0000256|HAMAP-Rule:MF_00185};
DE   AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=IPP transferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=IPPT {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=IPTase {ECO:0000256|HAMAP-Rule:MF_00185};
GN   Name=miaA {ECO:0000256|HAMAP-Rule:MF_00185,
GN   ECO:0000313|EMBL:CBJ79470.1};
GN   OrderedLocusNames=XBJ1_0320 {ECO:0000313|EMBL:CBJ79470.1};
OS   Xenorhabdus bovienii (strain SS-2004).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Xenorhabdus.
OX   NCBI_TaxID=406818 {ECO:0000313|EMBL:CBJ79470.1, ECO:0000313|Proteomes:UP000002045};
RN   [1] {ECO:0000313|Proteomes:UP000002045}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SS-2004 {ECO:0000313|Proteomes:UP000002045};
RA   Goodrich-Blair H., Barbazuk B., Bode H.B., Darby C., Du Z., Forst S.,
RA   Gaudriault S., Goldman B.S., Goodner B., Henkhaus J., Latreille P.,
RA   Medigue C., Miller N., Norton S., Ogier J.C., Rouy Z., Slater S.,
RA   Suen G.;
RT   "Complete genome sequence of Xenorhabdus nematophila (strain ATCC
RT   19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6).";
RL   Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CBJ79470.1, ECO:0000313|Proteomes:UP000002045}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SS-2004 {ECO:0000313|EMBL:CBJ79470.1,
RC   ECO:0000313|Proteomes:UP000002045};
RX   PubMed=22125637; DOI=10.1371/journal.pone.0027909;
RA   Chaston J.M., Suen G., Tucker S.L., Andersen A.W., Bhasin A., Bode E.,
RA   Bode H.B., Brachmann A.O., Cowles C.E., Cowles K.N., Darby C.,
RA   de Leon L., Drace K., Du Z., Givaudan A., Herbert Tran E.E.,
RA   Jewell K.A., Knack J.J., Krasomil-Osterfeld K.C., Kukor R., Lanois A.,
RA   Latreille P., Leimgruber N.K., Lipke C.M., Liu R., Lu X.,
RA   Martens E.C., Marri P.R., Medigue C., Menard M.L., Miller N.M.,
RA   Morales-Soto N., Norton S., Ogier J.C., Orchard S.S., Park D.,
RA   Park Y., Qurollo B.A., Sugar D.R., Richards G.R., Rouy Z.,
RA   Slominski B., Slominski K., Snyder H., Tjaden B.C., van der Hoeven R.,
RA   Welch R.D., Wheeler C., Xiang B., Barbazuk B., Gaudriault S.,
RA   Goodner B., Slater S.C., Forst S., Goldman B.S., Goodrich-Blair H.;
RT   "The entomopathogenic bacterial endosymbionts xenorhabdus and
RT   photorhabdus: convergent lifestyles from divergent genomes.";
RL   PLoS ONE 6:e27909-e27909(2011).
CC   -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC       adenine at position 37 in tRNAs that read codons beginning with
CC       uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC       (i(6)A). {ECO:0000256|HAMAP-Rule:MF_00185,
CC       ECO:0000256|RuleBase:RU003784}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(37) in tRNA + dimethylallyl diphosphate =
CC         diphosphate + N(6)-dimethylallyladenosine(37) in tRNA;
CC         Xref=Rhea:RHEA:26482, Rhea:RHEA-COMP:10162, Rhea:RHEA-
CC         COMP:10375, ChEBI:CHEBI:33019, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:74411, ChEBI:CHEBI:74415; EC=2.5.1.75;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00185,
CC         ECO:0000256|RuleBase:RU003783};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00185};
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00185}.
CC   -!- SIMILARITY: Belongs to the IPP transferase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00185, ECO:0000256|RuleBase:RU003785}.
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DR   EMBL; FN667741; CBJ79470.1; -; Genomic_DNA.
DR   STRING; 406818.XBJ1_0320; -.
DR   EnsemblBacteria; CBJ79470; CBJ79470; XBJ1_0320.
DR   KEGG; xbo:XBJ1_0320; -.
DR   eggNOG; ENOG4105DKX; Bacteria.
DR   eggNOG; COG0324; LUCA.
DR   HOGENOM; HOG000039996; -.
DR   KO; K00791; -.
DR   OMA; YAKRQFT; -.
DR   Proteomes; UP000002045; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0052381; F:tRNA dimethylallyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00185; IPP_trans; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR018022; IPT.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   Pfam; PF01715; IPPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00174; miaA; 1.
PE   3: Inferred from homology;
DR   PRODOM; D3UYU2.
DR   SWISS-2DPAGE; D3UYU2.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003785};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002045};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00185};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003785};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002045};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003785, ECO:0000313|EMBL:CBJ79470.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003783}.
FT   NP_BIND       2      9       ATP. {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   REGION        4      9       Substrate binding. {ECO:0000256|HAMAP-
FT                                Rule:MF_00185}.
FT   REGION       27     30       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   REGION      151    155       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   REGION      232    237       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   REGION      265    272       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   SITE         93     93       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   SITE        115    115       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
SQ   SEQUENCE   298 AA;  33317 MW;  197A945427F4847B CRC64;
     MGPTASGKTA LSIALHQHLP VELISVDSAL IYREMNIGTA KPTAAEQAQA PHRLVDILDP
     AEIYSAADFR RDALKEMADI TAAGRIPLLV GGTMLYFKAL LEGLSPLPSS NLEIRAQIEQ
     QAAKHGWEAL HRQLQEIDSV AAARIHPNDP QRLTRALEVF RISGKTLTAL TEMSGEILPY
     RVHQFAIAPA SREILHQRIA ARFEQMIKSG FEDEVKALYA RSDLHTDLPS IRCVGYRQMW
     SYLSGEISHD EMVYRGICAT RQLAKRQITW LRGWDNVTWL DSDQPEQALN TVMQVIST
//

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