(data stored in ACNUC1104 zone)

SWISSPROT: D3UZB0_XENBS

ID   D3UZB0_XENBS            Unreviewed;        92 AA.
AC   D3UZB0;
DT   20-APR-2010, integrated into UniProtKB/TrEMBL.
DT   20-APR-2010, sequence version 1.
DT   08-MAY-2019, entry version 58.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000256|HAMAP-Rule:MF_00413, ECO:0000256|SAAS:SAAS01077704};
DE   AltName: Full=Sulfur mediator TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur transfer protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000256|HAMAP-Rule:MF_00413};
GN   Name=sirA {ECO:0000313|EMBL:CBJ79692.1};
GN   Synonyms=tusA {ECO:0000256|HAMAP-Rule:MF_00413};
GN   OrderedLocusNames=XBJ1_0548 {ECO:0000313|EMBL:CBJ79692.1};
OS   Xenorhabdus bovienii (strain SS-2004).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Xenorhabdus.
OX   NCBI_TaxID=406818 {ECO:0000313|EMBL:CBJ79692.1, ECO:0000313|Proteomes:UP000002045};
RN   [1] {ECO:0000313|Proteomes:UP000002045}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SS-2004 {ECO:0000313|Proteomes:UP000002045};
RA   Goodrich-Blair H., Barbazuk B., Bode H.B., Darby C., Du Z., Forst S.,
RA   Gaudriault S., Goldman B.S., Goodner B., Henkhaus J., Latreille P.,
RA   Medigue C., Miller N., Norton S., Ogier J.C., Rouy Z., Slater S.,
RA   Suen G.;
RT   "Complete genome sequence of Xenorhabdus nematophila (strain ATCC
RT   19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6).";
RL   Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CBJ79692.1, ECO:0000313|Proteomes:UP000002045}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SS-2004 {ECO:0000313|EMBL:CBJ79692.1,
RC   ECO:0000313|Proteomes:UP000002045};
RX   PubMed=22125637; DOI=10.1371/journal.pone.0027909;
RA   Chaston J.M., Suen G., Tucker S.L., Andersen A.W., Bhasin A., Bode E.,
RA   Bode H.B., Brachmann A.O., Cowles C.E., Cowles K.N., Darby C.,
RA   de Leon L., Drace K., Du Z., Givaudan A., Herbert Tran E.E.,
RA   Jewell K.A., Knack J.J., Krasomil-Osterfeld K.C., Kukor R., Lanois A.,
RA   Latreille P., Leimgruber N.K., Lipke C.M., Liu R., Lu X.,
RA   Martens E.C., Marri P.R., Medigue C., Menard M.L., Miller N.M.,
RA   Morales-Soto N., Norton S., Ogier J.C., Orchard S.S., Park D.,
RA   Park Y., Qurollo B.A., Sugar D.R., Richards G.R., Rouy Z.,
RA   Slominski B., Slominski K., Snyder H., Tjaden B.C., van der Hoeven R.,
RA   Welch R.D., Wheeler C., Xiang B., Barbazuk B., Gaudriault S.,
RA   Goodner B., Slater S.C., Forst S., Goldman B.S., Goodrich-Blair H.;
RT   "The entomopathogenic bacterial endosymbionts xenorhabdus and
RT   photorhabdus: convergent lifestyles from divergent genomes.";
RL   PLoS ONE 6:e27909-e27909(2011).
CC   -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in
CC       the cell. Part of a sulfur-relay system required for 2-thiolation
CC       during synthesis of 2-thiouridine of the modified wobble base 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts
CC       with IscS and stimulates its cysteine desulfurase activity.
CC       Accepts an activated sulfur from IscS, which is then transferred
CC       to TusD, and thus determines the direction of sulfur flow from
CC       IscS to 2-thiouridine formation. Also appears to be involved in
CC       sulfur transfer for the biosynthesis of molybdopterin.
CC       {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- PATHWAY: tRNA modification. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- SUBUNIT: Interacts with IscS. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413,
CC       ECO:0000256|SAAS:SAAS00345499}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000256|HAMAP-Rule:MF_00413, ECO:0000256|SAAS:SAAS01077701}.
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DR   EMBL; FN667741; CBJ79692.1; -; Genomic_DNA.
DR   STRING; 406818.XBJ1_0548; -.
DR   EnsemblBacteria; CBJ79692; CBJ79692; XBJ1_0548.
DR   KEGG; xbo:XBJ1_0548; -.
DR   eggNOG; ENOG41082K8; Bacteria.
DR   eggNOG; COG0425; LUCA.
DR   HOGENOM; HOG000259291; -.
DR   KO; K04085; -.
DR   OMA; DAQGLRC; -.
DR   Proteomes; UP000002045; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
DR   PRODOM; D3UZB0.
DR   SWISS-2DPAGE; D3UZB0.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002045};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413,
KW   ECO:0000256|SAAS:SAAS00016049};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002045};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00413}.
FT   DOMAIN       20     44       UPF0033. {ECO:0000259|PROSITE:PS01148}.
FT   ACT_SITE     27     27       Cysteine persulfide intermediate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00413}.
SQ   SEQUENCE   92 AA;  10466 MW;  A448FE8057E2E125 CRC64;
     MFEPTEIVMS NVFANPDKTL DTQGLRCPEP VMMIRKIVRH MDAGQTLLII ADDPATTRDI
     PGFCRFMEHA LIAQETGQTP YRYLIKKNEN VA
//

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