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ID D1CDE4_THET1 Unreviewed; 690 AA.
AC D1CDE4;
DT 19-JAN-2010, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2010, sequence version 1.
DT 08-MAY-2019, entry version 67.
DE RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) {ECO:0000256|HAMAP-Rule:MF_00159};
DE EC=1.17.7.3 {ECO:0000256|HAMAP-Rule:MF_00159};
DE AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase {ECO:0000256|HAMAP-Rule:MF_00159};
GN Name=ispG {ECO:0000256|HAMAP-Rule:MF_00159};
GN OrderedLocusNames=Tter_0027 {ECO:0000313|EMBL:ACZ40950.1};
OS Thermobaculum terrenum (strain ATCC BAA-798 / YNP1).
OC Bacteria; Thermobaculum.
OX NCBI_TaxID=525904 {ECO:0000313|EMBL:ACZ40950.1, ECO:0000313|Proteomes:UP000000323};
RN [1] {ECO:0000313|EMBL:ACZ40950.1, ECO:0000313|Proteomes:UP000000323}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX PubMed=21304745;
RA Kiss H., Cleland D., Lapidus A., Lucas S., Del Rio T.G., Nolan M.,
RA Tice H., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N.,
RA Mavromatis K., Ovchinnikova G., Pati A., Chen A., Palaniappan K.,
RA Land M., Hauser L., Chang Y.J., Jeffries C.D., Lu M., Brettin T.,
RA Detter J.C., Goker M., Tindall B.J., Beck B., McDermott T.R.,
RA Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA Kyrpides N.C., Klenk H.P., Cheng J.F.;
RT "Complete genome sequence of 'Thermobaculum terrenum' type strain
RT (YNP1).";
RL Stand. Genomic Sci. 3:153-162(2010).
RN [2] {ECO:0000313|Proteomes:UP000000323}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX DOI=10.4056/sigs.1153107;
RA Kiss H., Cleland D., Lapidus A., Lucas S., Glavina Del Rio T.,
RA Nolan M., Tice H., Han C., Goodwin L., Pitluck S., Liolios K.,
RA Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A.,
RA Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Lu M.,
RA Brettin T., Detter J., Goker M., Tindall B., Beck B., McDermott T.,
RA Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P.,
RA Kyrpides N., Klenk H., Cheng J.;
RT "Complete genome sequence of Thermobaculum terrenum type strain
RT (YNP1T).";
RL Stand. Genomic Sci. 3:153-162(2010).
CC -!- FUNCTION: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate
CC (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
CC {ECO:0000256|HAMAP-Rule:MF_00159}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) +
CC H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic
CC diphosphate + reduced [flavodoxin]; Xref=Rhea:RHEA:43604,
CC Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:10623, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC ChEBI:CHEBI:58483, ChEBI:CHEBI:128753; EC=1.17.7.3;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00159};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00159};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|HAMAP-Rule:MF_00159};
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC deoxy-D-xylulose 5-phosphate: step 5/6. {ECO:0000256|HAMAP-
CC Rule:MF_00159, ECO:0000256|SAAS:SAAS00951740}.
CC -!- SIMILARITY: Belongs to the IspG family. {ECO:0000256|HAMAP-
CC Rule:MF_00159, ECO:0000256|SAAS:SAAS00951749}.
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DR EMBL; CP001825; ACZ40950.1; -; Genomic_DNA.
DR STRING; 525904.Tter_0027; -.
DR EnsemblBacteria; ACZ40950; ACZ40950; Tter_0027.
DR KEGG; ttr:Tter_0027; -.
DR eggNOG; ENOG4105C4E; Bacteria.
DR eggNOG; COG0821; LUCA.
DR HOGENOM; HOG000261018; -.
DR KO; K03526; -.
DR OMA; RIMSRYG; -.
DR BioCyc; TTER525904:G1GGS-27-MONOMER; -.
DR UniPathway; UPA00056; UER00096.
DR Proteomes; UP000000323; Chromosome 1.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046429; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.20.20.20; -; 2.
DR HAMAP; MF_00159; IspG; 1.
DR InterPro; IPR011005; Dihydropteroate_synth-like.
DR InterPro; IPR017178; IspG_atypical.
DR InterPro; IPR004588; IspG_bac-typ.
DR PANTHER; PTHR30454; PTHR30454; 1.
DR Pfam; PF04551; GcpE; 2.
DR PIRSF; PIRSF037336; IspG_like; 1.
DR SUPFAM; SSF51717; SSF51717; 1.
DR TIGRFAMs; TIGR00612; ispG_gcpE; 1.
PE 3: Inferred from homology;
DR PRODOM; D1CDE4.
DR SWISS-2DPAGE; D1CDE4.
KW 4Fe-4S {ECO:0000256|HAMAP-Rule:MF_00159,
KW ECO:0000256|SAAS:SAAS00951739};
KW Complete proteome {ECO:0000313|Proteomes:UP000000323};
KW Iron {ECO:0000256|HAMAP-Rule:MF_00159, ECO:0000256|SAAS:SAAS00951738};
KW Iron-sulfur {ECO:0000256|HAMAP-Rule:MF_00159,
KW ECO:0000256|SAAS:SAAS00951743};
KW Isoprene biosynthesis {ECO:0000256|HAMAP-Rule:MF_00159,
KW ECO:0000256|SAAS:SAAS00951733};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00159,
KW ECO:0000256|SAAS:SAAS00951748};
KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_00159,
KW ECO:0000256|SAAS:SAAS00951741, ECO:0000313|EMBL:ACZ40950.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000323}.
FT METAL 588 588 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT Rule:MF_00159}.
FT METAL 591 591 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT Rule:MF_00159}.
FT METAL 622 622 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT Rule:MF_00159}.
FT METAL 629 629 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT Rule:MF_00159}.
SQ SEQUENCE 690 AA; 76308 MW; 72DEDBBC0B80AF3E CRC64;
MVKSQEVTSN RYCVDPYSYK RRCTHEVMVG NVGVGGNNPI RVQSMTTTRT KDVESTVNQI
VSLARVGCEI VRVTTPTPAD ARAIKDIKKE LQSKNIHIPI VADVHFSPHA AMEAALWADK
VRINPGNFAD TKLFHIKEYT DEEYAKHLEK IAEKLYPLVM RCKERGISMR IGTNHGSLSD
RIMSRYGDTP LGMVESALEF VRMCEEWGYK DIILSMKASN PKVMIAAYRL LAARMLEYGM
TYPFHLGVTE AGNGEDGRIK SAIGIGTLLE DGIGDTVRVS LTEDPENEIP FAYELVKRYA
YGIVPDSDEA TSIDPLPDLR SPFDYSPRPT RKIFLGEHVL GANNPPLVLY KQSSPLSING
VVSINRPVGR DSLRADALSL EVVSEKDRDV FQTSASGLQF ASNKPALIAT LRFPVDVRDI
EAAAYMCSIE RGQEDRLSTV ARLAVEHKRP LVLELIANIA HDQARDLEGK HDYSVVDELI
SYALKVEQFG VNDLILALRI PDRSYRIRAY RLLDSRLREL GKDWNLLLTV TEDDLIDASI
GLGSLLCDGI GSMVSIEGNK PYEEKISLVF NILQASGARI SKAEFIACPS CGRTLFDLQT
TTEKIKSKTG HLKGVKIAVM GCIVNGLGEL ADADFGYMGS GEGKVNLFVG KTCVEKNVPE
EIAADKLVDL IKAHGRWIDP PDNNSSMHDS
//
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