(data stored in SCRATCH zone)

SWISSPROT: D1CDE4_THET1

ID   D1CDE4_THET1            Unreviewed;       690 AA.
AC   D1CDE4;
DT   19-JAN-2010, integrated into UniProtKB/TrEMBL.
DT   19-JAN-2010, sequence version 1.
DT   08-MAY-2019, entry version 67.
DE   RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) {ECO:0000256|HAMAP-Rule:MF_00159};
DE            EC=1.17.7.3 {ECO:0000256|HAMAP-Rule:MF_00159};
DE   AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase {ECO:0000256|HAMAP-Rule:MF_00159};
GN   Name=ispG {ECO:0000256|HAMAP-Rule:MF_00159};
GN   OrderedLocusNames=Tter_0027 {ECO:0000313|EMBL:ACZ40950.1};
OS   Thermobaculum terrenum (strain ATCC BAA-798 / YNP1).
OC   Bacteria; Thermobaculum.
OX   NCBI_TaxID=525904 {ECO:0000313|EMBL:ACZ40950.1, ECO:0000313|Proteomes:UP000000323};
RN   [1] {ECO:0000313|EMBL:ACZ40950.1, ECO:0000313|Proteomes:UP000000323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX   PubMed=21304745;
RA   Kiss H., Cleland D., Lapidus A., Lucas S., Del Rio T.G., Nolan M.,
RA   Tice H., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N.,
RA   Mavromatis K., Ovchinnikova G., Pati A., Chen A., Palaniappan K.,
RA   Land M., Hauser L., Chang Y.J., Jeffries C.D., Lu M., Brettin T.,
RA   Detter J.C., Goker M., Tindall B.J., Beck B., McDermott T.R.,
RA   Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P., Cheng J.F.;
RT   "Complete genome sequence of 'Thermobaculum terrenum' type strain
RT   (YNP1).";
RL   Stand. Genomic Sci. 3:153-162(2010).
RN   [2] {ECO:0000313|Proteomes:UP000000323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX   DOI=10.4056/sigs.1153107;
RA   Kiss H., Cleland D., Lapidus A., Lucas S., Glavina Del Rio T.,
RA   Nolan M., Tice H., Han C., Goodwin L., Pitluck S., Liolios K.,
RA   Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Lu M.,
RA   Brettin T., Detter J., Goker M., Tindall B., Beck B., McDermott T.,
RA   Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P.,
RA   Kyrpides N., Klenk H., Cheng J.;
RT   "Complete genome sequence of Thermobaculum terrenum type strain
RT   (YNP1T).";
RL   Stand. Genomic Sci. 3:153-162(2010).
CC   -!- FUNCTION: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate
CC       (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
CC       {ECO:0000256|HAMAP-Rule:MF_00159}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) +
CC         H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic
CC         diphosphate + reduced [flavodoxin]; Xref=Rhea:RHEA:43604,
CC         Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:10623, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:58483, ChEBI:CHEBI:128753; EC=1.17.7.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00159};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00159};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|HAMAP-Rule:MF_00159};
CC   -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC       biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC       deoxy-D-xylulose 5-phosphate: step 5/6. {ECO:0000256|HAMAP-
CC       Rule:MF_00159, ECO:0000256|SAAS:SAAS00951740}.
CC   -!- SIMILARITY: Belongs to the IspG family. {ECO:0000256|HAMAP-
CC       Rule:MF_00159, ECO:0000256|SAAS:SAAS00951749}.
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DR   EMBL; CP001825; ACZ40950.1; -; Genomic_DNA.
DR   STRING; 525904.Tter_0027; -.
DR   EnsemblBacteria; ACZ40950; ACZ40950; Tter_0027.
DR   KEGG; ttr:Tter_0027; -.
DR   eggNOG; ENOG4105C4E; Bacteria.
DR   eggNOG; COG0821; LUCA.
DR   HOGENOM; HOG000261018; -.
DR   KO; K03526; -.
DR   OMA; RIMSRYG; -.
DR   BioCyc; TTER525904:G1GGS-27-MONOMER; -.
DR   UniPathway; UPA00056; UER00096.
DR   Proteomes; UP000000323; Chromosome 1.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046429; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway; IEA:UniProtKB-UniPathway.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.20; -; 2.
DR   HAMAP; MF_00159; IspG; 1.
DR   InterPro; IPR011005; Dihydropteroate_synth-like.
DR   InterPro; IPR017178; IspG_atypical.
DR   InterPro; IPR004588; IspG_bac-typ.
DR   PANTHER; PTHR30454; PTHR30454; 1.
DR   Pfam; PF04551; GcpE; 2.
DR   PIRSF; PIRSF037336; IspG_like; 1.
DR   SUPFAM; SSF51717; SSF51717; 1.
DR   TIGRFAMs; TIGR00612; ispG_gcpE; 1.
PE   3: Inferred from homology;
DR   PRODOM; D1CDE4.
DR   SWISS-2DPAGE; D1CDE4.
KW   4Fe-4S {ECO:0000256|HAMAP-Rule:MF_00159,
KW   ECO:0000256|SAAS:SAAS00951739};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000323};
KW   Iron {ECO:0000256|HAMAP-Rule:MF_00159, ECO:0000256|SAAS:SAAS00951738};
KW   Iron-sulfur {ECO:0000256|HAMAP-Rule:MF_00159,
KW   ECO:0000256|SAAS:SAAS00951743};
KW   Isoprene biosynthesis {ECO:0000256|HAMAP-Rule:MF_00159,
KW   ECO:0000256|SAAS:SAAS00951733};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00159,
KW   ECO:0000256|SAAS:SAAS00951748};
KW   Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_00159,
KW   ECO:0000256|SAAS:SAAS00951741, ECO:0000313|EMBL:ACZ40950.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000323}.
FT   METAL       588    588       Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT                                Rule:MF_00159}.
FT   METAL       591    591       Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT                                Rule:MF_00159}.
FT   METAL       622    622       Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT                                Rule:MF_00159}.
FT   METAL       629    629       Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-
FT                                Rule:MF_00159}.
SQ   SEQUENCE   690 AA;  76308 MW;  72DEDBBC0B80AF3E CRC64;
     MVKSQEVTSN RYCVDPYSYK RRCTHEVMVG NVGVGGNNPI RVQSMTTTRT KDVESTVNQI
     VSLARVGCEI VRVTTPTPAD ARAIKDIKKE LQSKNIHIPI VADVHFSPHA AMEAALWADK
     VRINPGNFAD TKLFHIKEYT DEEYAKHLEK IAEKLYPLVM RCKERGISMR IGTNHGSLSD
     RIMSRYGDTP LGMVESALEF VRMCEEWGYK DIILSMKASN PKVMIAAYRL LAARMLEYGM
     TYPFHLGVTE AGNGEDGRIK SAIGIGTLLE DGIGDTVRVS LTEDPENEIP FAYELVKRYA
     YGIVPDSDEA TSIDPLPDLR SPFDYSPRPT RKIFLGEHVL GANNPPLVLY KQSSPLSING
     VVSINRPVGR DSLRADALSL EVVSEKDRDV FQTSASGLQF ASNKPALIAT LRFPVDVRDI
     EAAAYMCSIE RGQEDRLSTV ARLAVEHKRP LVLELIANIA HDQARDLEGK HDYSVVDELI
     SYALKVEQFG VNDLILALRI PDRSYRIRAY RLLDSRLREL GKDWNLLLTV TEDDLIDASI
     GLGSLLCDGI GSMVSIEGNK PYEEKISLVF NILQASGARI SKAEFIACPS CGRTLFDLQT
     TTEKIKSKTG HLKGVKIAVM GCIVNGLGEL ADADFGYMGS GEGKVNLFVG KTCVEKNVPE
     EIAADKLVDL IKAHGRWIDP PDNNSSMHDS
//

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