(data stored in SCRATCH zone)

SWISSPROT: D1CDJ7_THET1

ID   D1CDJ7_THET1            Unreviewed;       704 AA.
AC   D1CDJ7;
DT   19-JAN-2010, integrated into UniProtKB/TrEMBL.
DT   19-JAN-2010, sequence version 1.
DT   08-MAY-2019, entry version 63.
DE   RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump {ECO:0000256|HAMAP-Rule:MF_01129};
DE            EC=7.1.3.1 {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE            Short=H(+)-PPase {ECO:0000256|HAMAP-Rule:MF_01129};
GN   Name=hppA {ECO:0000256|HAMAP-Rule:MF_01129};
GN   OrderedLocusNames=Tter_0081 {ECO:0000313|EMBL:ACZ41003.1};
OS   Thermobaculum terrenum (strain ATCC BAA-798 / YNP1).
OC   Bacteria; Thermobaculum.
OX   NCBI_TaxID=525904 {ECO:0000313|EMBL:ACZ41003.1, ECO:0000313|Proteomes:UP000000323};
RN   [1] {ECO:0000313|EMBL:ACZ41003.1, ECO:0000313|Proteomes:UP000000323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX   PubMed=21304745;
RA   Kiss H., Cleland D., Lapidus A., Lucas S., Del Rio T.G., Nolan M.,
RA   Tice H., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N.,
RA   Mavromatis K., Ovchinnikova G., Pati A., Chen A., Palaniappan K.,
RA   Land M., Hauser L., Chang Y.J., Jeffries C.D., Lu M., Brettin T.,
RA   Detter J.C., Goker M., Tindall B.J., Beck B., McDermott T.R.,
RA   Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P., Cheng J.F.;
RT   "Complete genome sequence of 'Thermobaculum terrenum' type strain
RT   (YNP1).";
RL   Stand. Genomic Sci. 3:153-162(2010).
RN   [2] {ECO:0000313|Proteomes:UP000000323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX   DOI=10.4056/sigs.1153107;
RA   Kiss H., Cleland D., Lapidus A., Lucas S., Glavina Del Rio T.,
RA   Nolan M., Tice H., Han C., Goodwin L., Pitluck S., Liolios K.,
RA   Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Lu M.,
RA   Brettin T., Detter J., Goker M., Tindall B., Beck B., McDermott T.,
RA   Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P.,
RA   Kyrpides N., Klenk H., Cheng J.;
RT   "Complete genome sequence of Thermobaculum terrenum type strain
RT   (YNP1T).";
RL   Stand. Genomic Sci. 3:153-162(2010).
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force. {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC         Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01129};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01129};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01129}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase
CC       (TC 3.A.10) family. K(+)-insensitive subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01129}.
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DR   EMBL; CP001825; ACZ41003.1; -; Genomic_DNA.
DR   RefSeq; WP_012874038.1; NC_013525.1.
DR   STRING; 525904.Tter_0081; -.
DR   EnsemblBacteria; ACZ41003; ACZ41003; Tter_0081.
DR   KEGG; ttr:Tter_0081; -.
DR   eggNOG; ENOG4105EAY; Bacteria.
DR   eggNOG; COG3808; LUCA.
DR   HOGENOM; HOG000007098; -.
DR   KO; K15987; -.
DR   OMA; GWKGILF; -.
DR   OrthoDB; 98036at2; -.
DR   BioCyc; TTER525904:G1GGS-81-MONOMER; -.
DR   Proteomes; UP000000323; Chromosome 1.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0009678; F:hydrogen-translocating pyrophosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:1902600; P:proton transmembrane transport; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01129; PPase_energized_pump; 1.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
DR   PIRSF; PIRSF001265; H+-PPase; 1.
DR   TIGRFAMs; TIGR01104; V_PPase; 1.
PE   3: Inferred from homology;
DR   PRODOM; D1CDJ7.
DR   SWISS-2DPAGE; D1CDJ7.
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000323};
KW   Hydrogen ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Hydrolase {ECO:0000313|EMBL:ACZ41003.1};
KW   Ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000323};
KW   Translocase {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transmembrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01129}.
FT   TRANSMEM      6     30       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM     58     79       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM     91    118       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    138    162       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    174    192       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    275    297       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    309    330       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    336    355       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    393    411       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    423    444       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    489    507       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    519    540       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    588    606       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    612    632       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   SITE        483    483       Determinant of potassium independence.
FT                                {ECO:0000256|HAMAP-Rule:MF_01129}.
SQ   SEQUENCE   704 AA;  72928 MW;  BAD700134DAE8E1C CRC64;
     MSNEVLWAIL IFGAGILAVI FALSMARYVL SRDTGTPEMK AVADTIYEGA IAYLNRQYTT
     IASIAVITAI IIGAIIALVG SETMDLPGPQ AAIMTAIAFL AGALASGVSG YIAMLVAVKS
     NVRTAAAARR SLGDALLVSL RGGAAGAFFI IALSIMGVAA IFYAYNGFNQ PERAPFLIVG
     FGFGASFVAL FAQLGGGIYT KAADVGADLV GKVEAGIPED DPRNPAVIAD LVGDNVGDCA
     GRGADLFEST AAENIGAMIL GVSIFLATGR DNPGFIFLSL VLGAMGLIAS MIGVLSVRPS
     ANADPMGALN RGFAVTAVLS IIGLFIATLW VLDGNWWLFG CGLVGIITSI LFLLMTQYYT
     SEKYRPVRTI AEASKTGPAT NIISGIAIGF ENTALPAIVI SLAIGTAYFF GTRTGFSDNS
     FVNGVYGTAV ATMGMLMTVA YVLAMDIFGP ITDNAGGVVE MSNQPEEVRE RTDALDAVGN
     TTKALTKGYA IGSAALAAFL LFSAYLSDAR LEAVNLARVP VFIGGFLGGM LVFLFSSLAI
     RAVGRAAGDM IEEVRRQFRS EPGIMQGTVR PNYAKAVDIS TRAALREMVL PGILAVGMPV
     VVGLILRAEA VAALLMVGTM TGILVALVLN NGGGAWDNAK KLIESGQLLD EEGKPIGKGT
     ATHAAAVVGD TVGDPFKDTA GPSLHVLIKL LSTVTLVLAP LFIR
//

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