(data stored in SCRATCH zone)

SWISSPROT: D1CEV2_THET1

ID   D1CEV2_THET1            Unreviewed;       307 AA.
AC   D1CEV2;
DT   19-JAN-2010, integrated into UniProtKB/TrEMBL.
DT   19-JAN-2010, sequence version 1.
DT   08-MAY-2019, entry version 58.
DE   RecName: Full=Ribonuclease Z {ECO:0000256|HAMAP-Rule:MF_01818};
DE            Short=RNase Z {ECO:0000256|HAMAP-Rule:MF_01818};
DE            EC=3.1.26.11 {ECO:0000256|HAMAP-Rule:MF_01818};
DE   AltName: Full=tRNA 3 endonuclease {ECO:0000256|HAMAP-Rule:MF_01818};
DE   AltName: Full=tRNase Z {ECO:0000256|HAMAP-Rule:MF_01818};
GN   Name=rnz {ECO:0000256|HAMAP-Rule:MF_01818};
GN   OrderedLocusNames=Tter_0540 {ECO:0000313|EMBL:ACZ41458.1};
OS   Thermobaculum terrenum (strain ATCC BAA-798 / YNP1).
OC   Bacteria; Thermobaculum.
OX   NCBI_TaxID=525904 {ECO:0000313|EMBL:ACZ41458.1, ECO:0000313|Proteomes:UP000000323};
RN   [1] {ECO:0000313|EMBL:ACZ41458.1, ECO:0000313|Proteomes:UP000000323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX   PubMed=21304745;
RA   Kiss H., Cleland D., Lapidus A., Lucas S., Del Rio T.G., Nolan M.,
RA   Tice H., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N.,
RA   Mavromatis K., Ovchinnikova G., Pati A., Chen A., Palaniappan K.,
RA   Land M., Hauser L., Chang Y.J., Jeffries C.D., Lu M., Brettin T.,
RA   Detter J.C., Goker M., Tindall B.J., Beck B., McDermott T.R.,
RA   Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P., Cheng J.F.;
RT   "Complete genome sequence of 'Thermobaculum terrenum' type strain
RT   (YNP1).";
RL   Stand. Genomic Sci. 3:153-162(2010).
RN   [2] {ECO:0000313|Proteomes:UP000000323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-798 / YNP1 {ECO:0000313|Proteomes:UP000000323};
RX   DOI=10.4056/sigs.1153107;
RA   Kiss H., Cleland D., Lapidus A., Lucas S., Glavina Del Rio T.,
RA   Nolan M., Tice H., Han C., Goodwin L., Pitluck S., Liolios K.,
RA   Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Lu M.,
RA   Brettin T., Detter J., Goker M., Tindall B., Beck B., McDermott T.,
RA   Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P.,
RA   Kyrpides N., Klenk H., Cheng J.;
RT   "Complete genome sequence of Thermobaculum terrenum type strain
RT   (YNP1T).";
RL   Stand. Genomic Sci. 3:153-162(2010).
CC   -!- FUNCTION: Zinc phosphodiesterase, which displays some tRNA 3'-
CC       processing endonuclease activity. Probably involved in tRNA
CC       maturation, by removing a 3'-trailer from precursor tRNA.
CC       {ECO:0000256|HAMAP-Rule:MF_01818}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of RNA, removing extra 3'
CC         nucleotides from tRNA precursor, generating 3' termini of tRNAs.
CC         A 3'-hydroxy group is left at the tRNA terminus and a 5'-
CC         phosphoryl group is left at the trailer molecule.; EC=3.1.26.11;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01818};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01818};
CC       Note=Binds 2 Zn(2+) ions. {ECO:0000256|HAMAP-Rule:MF_01818};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01818}.
CC   -!- SIMILARITY: Belongs to the RNase Z family. {ECO:0000256|HAMAP-
CC       Rule:MF_01818}.
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DR   EMBL; CP001825; ACZ41458.1; -; Genomic_DNA.
DR   STRING; 525904.Tter_0540; -.
DR   EnsemblBacteria; ACZ41458; ACZ41458; Tter_0540.
DR   KEGG; ttr:Tter_0540; -.
DR   eggNOG; ENOG4105IES; Bacteria.
DR   eggNOG; COG1234; LUCA.
DR   HOGENOM; HOG000272419; -.
DR   KO; K00784; -.
DR   OMA; GTQRQMM; -.
DR   BioCyc; TTER525904:G1GGS-546-MONOMER; -.
DR   Proteomes; UP000000323; Chromosome 1.
DR   GO; GO:0042781; F:3'-tRNA processing endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   CDD; cd07717; RNaseZ_ZiPD-like_MBL-fold; 1.
DR   Gene3D; 3.60.15.10; -; 1.
DR   HAMAP; MF_01818; RNase_Z_BN; 1.
DR   InterPro; IPR001279; Metallo-B-lactamas.
DR   InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR   InterPro; IPR013471; RNase_Z/BN.
DR   Pfam; PF00753; Lactamase_B; 1.
DR   Pfam; PF12706; Lactamase_B_2; 1.
DR   SUPFAM; SSF56281; SSF56281; 1.
PE   3: Inferred from homology;
DR   PRODOM; D1CEV2.
DR   SWISS-2DPAGE; D1CEV2.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000323};
KW   Endonuclease {ECO:0000256|HAMAP-Rule:MF_01818};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01818,
KW   ECO:0000313|EMBL:ACZ41458.1};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_01818};
KW   Nuclease {ECO:0000256|HAMAP-Rule:MF_01818};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000323};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_01818};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_01818}.
FT   DOMAIN       21    134       Lactamase_B. {ECO:0000259|Pfam:PF00753}.
FT   DOMAIN      199    271       Lactamase_B. {ECO:0000259|Pfam:PF12706}.
FT   ACT_SITE     65     65       Proton acceptor. {ECO:0000256|HAMAP-Rule:
FT                                MF_01818}.
FT   METAL        61     61       Zinc 1; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
FT   METAL        63     63       Zinc 1; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
FT   METAL        65     65       Zinc 2; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
FT   METAL        66     66       Zinc 2; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
FT   METAL       140    140       Zinc 1; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
FT   METAL       208    208       Zinc 1; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
FT   METAL       208    208       Zinc 2; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
FT   METAL       270    270       Zinc 2; catalytic. {ECO:0000256|HAMAP-
FT                                Rule:MF_01818}.
SQ   SEQUENCE   307 AA;  33924 MW;  E6E9E955C9F78C1C CRC64;
     MLDVTLLGTS GTLPLPGRYL SSALIRIGGK LFLIDCGEGT QVALRAKGWG IRRIDSILLT
     HSHADHVLGL PGLLLTLAFS QRGAEEPLRI FGPPSVLPVL ESIRVFAPRL PYPVYFIPLE
     VGDEVDVTDN LKLFAGPTEH DVPCLSYSLY VPRSPKFDVE RANSLGVPRD LWGRLQRGES
     VFVGEREIQP QDVLGPPRKG LKLVFITDTR FTEDLVSFAS KIQPPDLLIS EGMYGDESMR
     PKDWDVPHMT FAEAAKVASA SGARLLWLTH YSPMMADPSR YIENARRIFP NAIAAQDGTE
     ISLSYED
//

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