(data stored in SCRATCH zone)

SWISSPROT: D1YVJ6_METPS

ID   D1YVJ6_METPS            Unreviewed;       636 AA.
AC   D1YVJ6;
DT   09-FEB-2010, integrated into UniProtKB/TrEMBL.
DT   09-FEB-2010, sequence version 1.
DT   11-DEC-2019, entry version 60.
DE   RecName: Full=Threonine--tRNA ligase {ECO:0000256|HAMAP-Rule:MF_00184};
DE            EC=6.1.1.3 {ECO:0000256|HAMAP-Rule:MF_00184};
DE   AltName: Full=Threonyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00184};
DE            Short=ThrRS {ECO:0000256|HAMAP-Rule:MF_00184};
GN   Name=thrS {ECO:0000256|HAMAP-Rule:MF_00184,
GN   ECO:0000313|EMBL:BAI60468.1};
GN   OrderedLocusNames=MCP_0396 {ECO:0000313|EMBL:BAI60468.1};
OS   Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 /
OS   SANAE).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanocellales; Methanocellaceae; Methanocella.
OX   NCBI_TaxID=304371 {ECO:0000313|EMBL:BAI60468.1, ECO:0000313|Proteomes:UP000001882};
RN   [1] {ECO:0000313|Proteomes:UP000001882}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17711 / JCM 13418 / NBRC 101707 / SANAE
RC   {ECO:0000313|Proteomes:UP000001882};
RX   PubMed=21829548; DOI=10.1371/journal.pone.0022898;
RA   Sakai S., Takaki Y., Shimamura S., Sekine M., Tajima T., Kosugi H.,
RA   Ichikawa N., Tasumi E., Hiraki A.T., Shimizu A., Kato Y., Nishiko R.,
RA   Mori K., Fujita N., Imachi H., Takai K.;
RT   "Genome sequence of a mesophilic hydrogenotrophic methanogen Methanocella
RT   paludicola, the first cultivated representative of the order
RT   Methanocellales.";
RL   PLoS ONE 6:E22898-E22898(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-
CC         threonyl-tRNA(Thr); Xref=Rhea:RHEA:24624, Rhea:RHEA-COMP:9670,
CC         Rhea:RHEA-COMP:9704, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57926, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78534, ChEBI:CHEBI:456215; EC=6.1.1.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00184};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00184};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00184};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00184}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00184}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00184}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|HAMAP-Rule:MF_00184}.
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DR   EMBL; AP011532; BAI60468.1; -; Genomic_DNA.
DR   STRING; 304371.MCP_0396; -.
DR   EnsemblBacteria; BAI60468; BAI60468; MCP_0396.
DR   KEGG; mpd:MCP_0396; -.
DR   PATRIC; fig|304371.9.peg.406; -.
DR   eggNOG; arCOG00401; Archaea.
DR   eggNOG; COG0441; LUCA.
DR   HOGENOM; HOG000073571; -.
DR   KO; K01868; -.
DR   OMA; SCKGHPL; -.
DR   Proteomes; UP000001882; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.800; -; 1.
DR   Gene3D; 3.50.80.10; -; 1.
DR   HAMAP; MF_00184; Thr_tRNA_synth; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR023509; DTD-like_sf.
DR   InterPro; IPR002320; Thr-tRNA-ligase_IIa.
DR   InterPro; IPR015011; Threonyl-tRNA_syn_edit_dom_arc.
DR   PANTHER; PTHR11451; PTHR11451; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF08915; tRNA-Thr_ED; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
DR   PRODOM; D1YVJ6.
DR   SWISS-2DPAGE; D1YVJ6.
KW   Aminoacyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00184,
KW   ECO:0000313|EMBL:BAI60468.1};
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00184};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00184};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00184};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00184};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00184};
KW   Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00184};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001882};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_00184};
KW   tRNA-binding {ECO:0000256|HAMAP-Rule:MF_00184};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00184}.
FT   DOMAIN          208..508
FT                   /note="AA_TRNA_LIGASE_II"
FT                   /evidence="ECO:0000259|PROSITE:PS50862"
FT   METAL           304
FT                   /note="Zinc; catalytic"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00184"
FT   METAL           356
FT                   /note="Zinc; catalytic"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00184"
FT   METAL           480
FT                   /note="Zinc; catalytic"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00184"
SQ   SEQUENCE   636 AA;  72792 MW;  A88654C124DA5553 CRC64;
     MQLLLIHSDF IEYEVKKSTP VAEKIGDDLK SGRMEDALAA FITVEKPDEA NPQYVIDRGV
     ESIVKMAEQV KATRVMLYPY AHLSSNLSSP KTAIEVLTGL EAALKAKNFE VKRSPFGWYK
     AFTVKCKGHP LSELSRNIVP EGSTINICAG EKPEVVSEAV KNEEKLKSYF YVMDVDGTLK
     DPKTFDYKGH EKLKAFVDYE MAKKRAVDRV PPHVELMRRL ELADYEPGSD PGNMRFYPKG
     RMVKNLLENY VLMEAAKKGA MEVETPIMYD MEHPTLKKYL DRFPARQYTV IADKKNLFLR
     FAACFGQFLM SHDMTISYKN LPLKMIELTR YSFRKEQRGE LVGLRRLRAF TMPDMHTLCA
     DMAGATSEFR DQYEMCINTL DTIGLTLKDY EVAIRFTRDF YDQNKGFIKG LVKRAGKPVV
     IEMWDQRFFY FVLKFEFNFI DSLDKASALS TVQIDVENAE RYDITYVDQD GSKKYPTILH
     CSPSGAIERC IYAQLEKQFM ESEKGQVPML PVWLSPTQVR IISLSDRHAD YCRRLAGELR
     PNRVDIDDRE ETVSKKVRDA GKEWIPYVVT IGDAEIGKDK FPVVVRSESQ PNKPLKVEMT
     VEELKARLAK DTDALPARPL PIAESLAKRP KFVGSI
//

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