(data stored in SCRATCH zone)

SWISSPROT: D1YW74_METPS

ID   D1YW74_METPS            Unreviewed;       340 AA.
AC   D1YW74;
DT   09-FEB-2010, integrated into UniProtKB/TrEMBL.
DT   09-FEB-2010, sequence version 1.
DT   11-DEC-2019, entry version 45.
DE   RecName: Full=Radical_SAM domain-containing protein {ECO:0000259|Pfam:PF04055};
GN   OrderedLocusNames=MCP_0624 {ECO:0000313|EMBL:BAI60696.1};
OS   Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 /
OS   SANAE).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanocellales; Methanocellaceae; Methanocella.
OX   NCBI_TaxID=304371 {ECO:0000313|EMBL:BAI60696.1, ECO:0000313|Proteomes:UP000001882};
RN   [1] {ECO:0000313|Proteomes:UP000001882}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17711 / JCM 13418 / NBRC 101707 / SANAE
RC   {ECO:0000313|Proteomes:UP000001882};
RX   PubMed=21829548; DOI=10.1371/journal.pone.0022898;
RA   Sakai S., Takaki Y., Shimamura S., Sekine M., Tajima T., Kosugi H.,
RA   Ichikawa N., Tasumi E., Hiraki A.T., Shimizu A., Kato Y., Nishiko R.,
RA   Mori K., Fujita N., Imachi H., Takai K.;
RT   "Genome sequence of a mesophilic hydrogenotrophic methanogen Methanocella
RT   paludicola, the first cultivated representative of the order
RT   Methanocellales.";
RL   PLoS ONE 6:E22898-E22898(2011).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|PIRSR:PIRSR004869-50};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000256|PIRSR:PIRSR004869-50};
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DR   EMBL; AP011532; BAI60696.1; -; Genomic_DNA.
DR   STRING; 304371.MCP_0624; -.
DR   EnsemblBacteria; BAI60696; BAI60696; MCP_0624.
DR   KEGG; mpd:MCP_0624; -.
DR   PATRIC; fig|304371.9.peg.643; -.
DR   eggNOG; arCOG00946; Archaea.
DR   eggNOG; COG1180; LUCA.
DR   HOGENOM; HOG000227597; -.
DR   KO; K04069; -.
DR   OMA; YHFYPGS; -.
DR   BioCyc; MPAL304371:GI7G-640-MONOMER; -.
DR   Proteomes; UP000001882; Chromosome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR027596; AmmeMemoSam_rS.
DR   InterPro; IPR034457; Organic_radical-activating.
DR   InterPro; IPR016431; Pyrv-formate_lyase-activ_prd.
DR   InterPro; IPR007197; rSAM.
DR   PANTHER; PTHR30352; PTHR30352; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   PIRSF; PIRSF004869; PflX_prd; 1.
DR   SFLD; SFLDG01101; Uncharacterised_Radical_SAM_Su; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   TIGRFAMs; TIGR04337; AmmeMemoSam_rS; 1.
PE   4: Predicted;
DR   PRODOM; D1YW74.
DR   SWISS-2DPAGE; D1YW74.
KW   Iron {ECO:0000256|PIRSR:PIRSR004869-50};
KW   Iron-sulfur {ECO:0000256|PIRSR:PIRSR004869-50};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR004869-50};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001882};
KW   S-adenosyl-L-methionine {ECO:0000256|PIRSR:PIRSR004869-50}.
FT   DOMAIN          80..231
FT                   /note="Radical_SAM"
FT                   /evidence="ECO:0000259|Pfam:PF04055"
FT   METAL           83
FT                   /note="Iron-sulfur (4Fe-4S-S-AdoMet)"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
FT   METAL           87
FT                   /note="Iron-sulfur (4Fe-4S-S-AdoMet)"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
FT   METAL           90
FT                   /note="Iron-sulfur (4Fe-4S-S-AdoMet)"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
SQ   SEQUENCE   340 AA;  38923 MW;  CF7C59769009C675 CRC64;
     MLKEAILWEP LKDGKAKCNV CSYRCVILPG KLGHCRTRKN IDGKVFSLIY GTVTSEASDP
     IEKKPLYHFY PGSYSYSVGS VGCNFQCEHC QNWGISQAEI QEVYTQDIMP EELARNAVDT
     LCKSVSWTYN EPGIWVEYSH DCAVKCHEKD IKTIYVTNGY PTPEHLDVMK GLLDAFRVDI
     KAFSEDFYKK ICKSKLEPVL QSTKMAKDMG MHIEVVNLVI PGQNDSPEEI RALSKWCFDN
     LGPDTPIHFT RFHPMYHMDH LESTPIVVLE RAHDIAKKEG MNYVYLGNTL GNKYENTWCP
     KCNELLIERY GFQIMRYSIT PDKKCPKCGE KIPIVGDYGR
//

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